<abstract xmlns="http://www.w3.org/1999/xhtml">

<bold>Aim of the study:</bold> The aim of this study is to determine the values of salivary enzyme biomarkers (alkaline phosphatase – ALP, aspartate aminotransferase – AST and lactate dehydrogenase – LDH) in subjects with healthy and diseased periodontium and to investigate the possibility of using these salivary enzymes as diagnostic and prognostic markers.
<bold>Methods:</bold> We collected saliva with the spitting method from all examinees in the morning, using the recommendations provided by <italic>Navazesh.</italic> The values of the enzymes in saliva were determined spectro-photometrically, with the following methods: ALP-IFCC, AST-IFCC, LDH-PYRUVATE. IGI Silness-Löe was used to determine the presence of gingival inflammation, and to determine the presence of clinically manifest periodontitis, we determined the clinical loss of periodontal attachment with a graduated periodontal probe. For statistical purposes, we used the method of ANOVA Chi Square and Student’s t-test.
<bold>Results:</bold> The difference in the average salivary AST and LDH values between the first and the second group, as well between the first and third group is statistically significant (p &lt; 0.000). The difference in the average salivary AST and LDH values between the examinees with gingivitis and the examinees with clinically manifest periodontal disease is statistically insignificant (p &lt; 0.485101 for AST, p &lt; 0.816665 for LDH). The difference in the average salivary levels of ALP between the three groups is statistically significant (p &lt; 0.000).
<bold>Conclusion:</bold> The salivary levels of AST, LDH, and ALP can be used as diagnostic markers, while ALP can also be used as a prognostic marker for periodontal disease.
</abstract>

Aim of the study: The aim of this study is to determine the values of salivary enzyme biomarkers (alkaline phosphatase – ALP, aspartate aminotransferase – AST and lactate dehydrogenase – LDH) in subjects with healthy and diseased periodontium and to investigate the possibility of using these salivary enzymes as diagnostic and prognostic markers.
Methods: We collected saliva with the spitting method from all examinees in the morning, using the recommendations provided by Navazesh. The values of the enzymes in saliva were determined spectro-photometrically, with the following methods: ALP-IFCC, AST-IFCC, LDH-PYRUVATE. IGI Silness-Löe was used to determine the presence of gingival inflammation, and to determine the presence of clinically manifest periodontitis, we determined the clinical loss of periodontal attachment with a graduated periodontal probe. For statistical purposes, we used the method of ANOVA Chi Square and Student’s t-test.
Results: The difference in the average salivary AST and LDH values between the first and the second group, as well between the first and third group is statistically significant (p < 0.000). The difference in the average salivary AST and LDH values between the examinees with gingivitis and the examinees with clinically manifest periodontal disease is statistically insignificant (p < 0.485101 for AST, p < 0.816665 for LDH). The difference in the average salivary levels of ALP between the three groups is statistically significant (p < 0.000).
Conclusion: The salivary levels of AST, LDH, and ALP can be used as diagnostic markers, while ALP can also be used as a prognostic marker for periodontal disease.

Diagnostic and Prognostic Markers of Periodontal Disease

Ss. Cyril and Methodius University in Skopje, Faculty of Dentistry – Skopje

PRILOZI

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"Diagnostic and Prognostic Markers of Periodontal Disease"}

Aim of the study: The aim of this study is to determine the values of salivary enzyme biomarkers (alkaline phosphatase – ALP, aspartate aminotransferase – AST...

Diagnostic and Prognostic Markers of Periodontal Disease

Publicado en línea: 30 dic 2021

Páginas: 89 - 95

DOI: https://doi.org/10.2478/prilozi-2021-0039

Palabras clave
periodontal disease, saliva, aspartate aminotransferase, lactate dehydrogenase, alkaline phosphatase

© 2021 Ioannis Chrysikos, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Diagnostic and Prognostic Markers of Periodontal Disease

Publicado en línea: 30 dic 2021

Páginas: 89 - 95

DOI: https://doi.org/10.2478/prilozi-2021-0039

Palabras claveperiodontal disease, saliva, aspartate aminotransferase, lactate dehydrogenase, alkaline phosphatase

© 2021 Ioannis Chrysikos, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
periodontal disease, saliva, aspartate aminotransferase, lactate dehydrogenase, alkaline phosphatase