<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In the event of an epidemic of Legionnaires’ disease, prompt and unambiguous identification of the source of infection and immediate undertaking of repair actions is a necessary condition to limit and minimise the effects of the developing epidemic. In the classical method for determining the level of <italic>Legionella</italic> bacteria in water samples, the effectiveness of the reparative action (increase of the water temperature in the water supply system to 600C, additional chlorination) can only be confirmed after 14 days!!! Only by using the IMMS&amp;FCM method can <italic>Legionella’s</italic> determination time be reduced to 2-4 hours, which is the most important factor in limiting the development of an epidemic.</p></abstract>

In the event of an epidemic of Legionnaires’ disease, prompt and unambiguous identification of the source of infection and immediate undertaking of repair actions is a necessary condition to limit and minimise the effects of the developing epidemic. In the classical method for determining the level of Legionella bacteria in water samples, the effectiveness of the reparative action (increase of the water temperature in the water supply system to 600C, additional chlorination) can only be confirmed after 14 days!!! Only by using the IMMS&FCM method can Legionella’s determination time be reduced to 2-4 hours, which is the most important factor in limiting the development of an epidemic.

Developing a Methodology for Testing and Preliminary Determination of the Presence of <italic>Legionella Spp.</italic> and <italic>Legionella Pneumophila</italic> in Environmental Water Samples by Immunomagnetic Separation Combined with Flow Cytometry

Maritime & Hyperbaric Medicine Department

Polish Hyperbaric Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Developing a Methodology for Testing and Preliminary Determination of the Presence of  and  in Environmental Water Samples by Immunomagnetic Separation Combined with Flow Cytometry

In the event of an epidemic of Legionnaires’ disease, prompt and unambiguous identification of the source of infection and immediate undertaking of repair...

Developing a Methodology for Testing and Preliminary Determination of the Presence of Legionella Spp. and Legionella Pneumophila in Environmental Water Samples by Immunomagnetic Separation Combined with Flow Cytometry

Publicado en línea: 10 ene 2020

Páginas: 71 - 92

Recibido: 22 feb 2019

Aceptado: 23 jul 2019

DOI: https://doi.org/10.2478/phr-2019-0013

Palabras clave
Legionella spp., Legionnaires’ Disease, immunomagnetic separation, flow cytometry, hot water distribution systems, public utility buildings, ships

© 2019 Zbigniew Dąbrowiecki et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Developing a Methodology for Testing and Preliminary Determination of the Presence of Legionella Spp. and Legionella Pneumophila in Environmental Water Samples by Immunomagnetic Separation Combined with Flow Cytometry

Publicado en línea: 10 ene 2020

Páginas: 71 - 92

Recibido: 22 feb 2019

Aceptado: 23 jul 2019

DOI: https://doi.org/10.2478/phr-2019-0013

Palabras claveLegionella spp., Legionnaires’ Disease, immunomagnetic separation, flow cytometry, hot water distribution systems, public utility buildings, ships

© 2019 Zbigniew Dąbrowiecki et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Palabras clave
Legionella spp., Legionnaires’ Disease, immunomagnetic separation, flow cytometry, hot water distribution systems, public utility buildings, ships