<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Ionizing radiation (IR) is applied to inactivate the nuclear genome in rainbow trout eggs during induced androgenetic development. However, IR-generated reactive oxygen species (ROS) may affect developmental potential of eggs and reduce the effectiveness of androgenesis. To verify this assumption, androgenetic development of rainbow trout was induced in eggs irradiated with 350 Gy of X-rays. Survival rates, pH of the ovarian fluid and activity of antioxidant enzymes, including SOD, CAT and GPx, were examined in non-irradiated and irradiated eggs originating from four females. Survival rates of androgenetic embryos developing in eggs produced by different females varied from 1% to 57% and these inter-clutch differences were significant. Eggs from female F4, which showed the highest developmental competence for androgenesis, also showed increased activities of SOD, CAT and GPx enzymes. The pH value of the ovarian fluid of each female was over 8 before and after irradiation, therefore it seems that radiation did not affect the ovarian fluid pH. Considering the above-mentioned inter-clutch differences, a strong maternal effect on the effectiveness of androgenesis can be assumed. Eggs with increased activity of antioxidant enzymes before irradiation should be expected to show increased developmental competence for androgenesis.</p>
</abstract>

Ionizing radiation (IR) is applied to inactivate the nuclear genome in rainbow trout eggs during induced androgenetic development. However, IR-generated reactive oxygen species (ROS) may affect developmental potential of eggs and reduce the effectiveness of androgenesis. To verify this assumption, androgenetic development of rainbow trout was induced in eggs irradiated with 350 Gy of X-rays. Survival rates, pH of the ovarian fluid and activity of antioxidant enzymes, including SOD, CAT and GPx, were examined in non-irradiated and irradiated eggs originating from four females. Survival rates of androgenetic embryos developing in eggs produced by different females varied from 1% to 57% and these inter-clutch differences were significant. Eggs from female F4, which showed the highest developmental competence for androgenesis, also showed increased activities of SOD, CAT and GPx enzymes. The pH value of the ovarian fluid of each female was over 8 before and after irradiation, therefore it seems that radiation did not affect the ovarian fluid pH. Considering the above-mentioned inter-clutch differences, a strong maternal effect on the effectiveness of androgenesis can be assumed. Eggs with increased activity of antioxidant enzymes before irradiation should be expected to show increased developmental competence for androgenesis.

Inter-clutch egg differences and androgenesis in rainbow trout (<italic>Oncorhynchus mykiss</italic>, Walbaum 1792)

Faculty of Oceanography and Geography, Institute of Oceanography, Division of Marine Biology and Ecology

Faculty of Oceanography and Geography, Institute of Oceanography, Division of Marine Biotechnology

Department of the Salmonid Research in Rutki

Oceanological and Hydrobiological Studies

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Inter-clutch egg differences and androgenesis in rainbow trout (, Walbaum 1792)

Ionizing radiation (IR) is applied to inactivate the nuclear genome in rainbow trout eggs during induced androgenetic development. However, IR-generated...

Group	Eyed stage	Swim-up stage
C1	99.14 ± 0.56	96.24 ± 2.21
C2	95.51 ± 0.49	93.29 ± 2.20
C3	95.08 ± 3.57	93.83 ± 4.07
C4	93.77 ± 1.01	93.14 ± 1.18
aH1	46.67	-
aH2	3,03	-
aH3	69.14	-
aH4	67.83	-
aDH1	29,35 ± 2.38	7.60 ± 0.38
aDH2	4,10 ± 1.01	1.39 ± 1.05
aDH3	1,39 ± 0.50	0.24 ± 0.27
aDH4	57,14 ± 1.80	27.50 ± 0.95

Female	Groups
Female	non-irradiated	irradiated
F1	4.73 ± 0.17 *	4.71 ± 0.11
F2	4.75 ± 0.17 *	4.84 ± 0.12
F3	5.02 ± 0.12	4.98 ± 0.16
F4	4.01 ± 0.21	3.99 ± 0.18

Inter-clutch egg differences and androgenesis in rainbow trout (Oncorhynchus mykiss, Walbaum 1792)

Article Category: Original research paper

Publicado en línea: 05 jun 2021

Páginas: 160 - 168

Recibido: 14 sept 2020

Aceptado: 16 nov 2020

DOI: https://doi.org/10.2478/oandhs-2021-0015

Palabras clave
androgenesis, antioxidant enzymes, egg quality, ionizing radiation, reactive oxygen species,

© 2021 Marcin Polonis et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1a-c

Figure 2

Survival rates (%, averages ± SD) of rainbow trout from control (C), androgenetic haploid (aH) and androgenetic diploid (aDH) groups

Comparison of diameters (mm, averages ± SD) of irradiated and non-irradiated eggs of rainbow trout. An asterisk indicates lack of significant differences (p < 0.05) between non-irradiated eggs from females F1 and F2.

Inter-clutch egg differences and androgenesis in rainbow trout (Oncorhynchus mykiss, Walbaum 1792)

Article Category: Original research paper

Publicado en línea: 05 jun 2021

Páginas: 160 - 168

Recibido: 14 sept 2020

Aceptado: 16 nov 2020

DOI: https://doi.org/10.2478/oandhs-2021-0015

Palabras claveandrogenesis, antioxidant enzymes, egg quality, ionizing radiation, reactive oxygen species,

© 2021 Marcin Polonis et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1a-c

Figure 2

Survival rates (%, averages ± SD) of rainbow trout from control (C), androgenetic haploid (aH) and androgenetic diploid (aDH) groups

Comparison of diameters (mm, averages ± SD) of irradiated and non-irradiated eggs of rainbow trout. An asterisk indicates lack of significant differences (p < 0.05) between non-irradiated eggs from females F1 and F2.

Palabras clave
androgenesis, antioxidant enzymes, egg quality, ionizing radiation, reactive oxygen species,