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Deuterium isotope effects in mechanistic studies of biotransformations of l-tyrosine and p-hydroxyphenylpyruvic acid catalyzed by the enzyme l-phenylalanine dehydrogenase

Katarzyna Pałka

Katarzyna Pałka

Department of Chemistry, University of WarsawWarsaw, Poland
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Pałka, Katarzyna
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Katarzyna Podsadni

Katarzyna Podsadni

Department of Biochemistry, Medical Faculty, Medical University of WarsawWarsaw, Poland
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Podsadni, Katarzyna
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Jolanta Szymańska-Majchrzak

Jolanta Szymańska-Majchrzak

Department of Biochemistry, Medical Faculty, Medical University of WarsawWarsaw, Poland
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Szymańska-Majchrzak, Jolanta
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Elżbieta Winnicka

Elżbieta Winnicka

Department of Chemistry, University of WarsawWarsaw, Poland
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Winnicka, Elżbieta
  
02 may 2025
Deuterium isotope effects in mechanistic studies of biotransformations of l-tyrosine and p-hydroxyphenylpyruvic acid catalyzed by the enzyme l-phenylalanine dehydrogenase's Cover Image
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Categoría del artículo: Technical Note
Publicado en línea: 02 may 2025
Páginas: 51 - 56
Recibido: 05 ene 2024
Aceptado: 04 mar 2025
DOI: https://doi.org/10.2478/nuka-2025-0006
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© 2025 Katarzyna Pałka et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Enforced metabolic pathways of accumulated l-Phe and l-Tyr in a person suffering from PKU and type I tyrosinemia diseases.
Enforced metabolic pathways of accumulated l-Phe and l-Tyr in a person suffering from PKU and type I tyrosinemia diseases.

Fig. 2.

Alternative metabolic pathways of l-Tyr in a person suffering from type II tyrosinemia disease.
Alternative metabolic pathways of l-Tyr in a person suffering from type II tyrosinemia disease.

Fig. 3.

Reversible conversion of l-Phe and l-Tyr into corresponding oxoacids catalyzed by the enzyme PheDH.
Reversible conversion of l-Phe and l-Tyr into corresponding oxoacids catalyzed by the enzyme PheDH.

Solvent isotope effects (SIE) and kinetic isotope effects (KIE) in the oxidative deamination of l-Tyr and reductive amination of PPA catalyzed by enzyme PheDH

Reactant SIE
on Vmax on Vmax/KM
l-Tyr 1.43 ± 0.09 1.48 ± 0.12
PPA 1.44 ± 0.08 1.58 ± 0.14
KIE
on Vmax on Vmax/KM
l-Tyr/[2-2H]-l-Tyr 2.26 ± 0.10 2.87 ± 0.11
PPA/[(3S)-2H]-PPA 1.55 ± 0.06 1.53 ± 0.16
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Inglés
Calendario de la edición:
4 veces al año
Temas de la revista:
Química, Química nuclear, Física, Astronomía y astrofísica, Física, otros
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