An Eva Green Real-Time PCR Assay for Porcine DNA Analysis in Raw and Processed Foods

[1] S. Soares, J. S. Amaral, M. B. P. P. Oliveira, and I. Mafra, “A SYBR Green real-time PCR assay to detect and quantify pork meat in processed poultry meat products”, Meat Sci. Vol. 94, Pp. 115–120, 2013. DOI: 10.1016/j.meatsci.2012.12.01223403303 Open DOISearch in Google Scholar

[2] H. Lubis, N.T. Salihah, M.M. Hossain, and M. U. Ahmed, “Development of fast and sensitive real-time qPCR assay based on a novel probe for detection of porcine DNA in food sample”, LWT - Food Sci. and Technol., Vol. 84, Pp. 686–692, 2017. DOI:10.1016/j.lwt.2017.06.043 Open DOISearch in Google Scholar

[3] L.M. Chiş, and D.C. Vodnar, “Detection of the species of origin for pork, chicken and beef in meat food products by real-time PCR”, Saf., 5, 2019, DOI: 10.3390/safety5040083 Open DOISearch in Google Scholar

[4] H.N. Lubis, N.F. Mohd-Naim, N.N. Alizul, and M.U. Ahmed, “From market to food plate: Current trusted technology and innovations in halal food analysis”, Trends in Food Sci. and Technol., Vol. 58, Pp. 55–68, 2016. DOI: 10.1016/j.tifs.2016.10.024 Open DOISearch in Google Scholar

[5] H. Munirah, J.L.Z. Ying, S. Roy, I.A. Rahman, and M.U. Ahmed, “Rapid detection of pork DNA in food samples using reusable electrochemical sensor”, Scientia Bruneiana, Vol. 15, Pp. 91-98, 2016. DOI: 10.46537/scibru.v15i0.36 Open DOISearch in Google Scholar

[6] S. Roy, I.A. Rahman, and M.U. Ahmed, “Paper-based rapid detection of pork and chicken using LAMP-magnetic bead aggregates”, Anal. Methods., Vol. 8, Pp. 2391-2399, 2016. DOI: 10.1039/C6AY00274A Open DOISearch in Google Scholar

[7] S. Roy, I.A. Rahman, J.H. Santos, and M.U. Ahmed, “Meat species identification using DNA-redox electrostatic interactions and non-specific adsorption on graphene biochips”, Food Control., Vol. 61, Pp. 70-78, 2016. DOI: 10.1016/j.foodcont.2015.09.029 Open DOISearch in Google Scholar

[8] Y. Erwanto, A. Rohman, L. Arsyanti, and Y. Pranoto, “Identification of pig DNA in food products using polymerase chain reaction (PCR) for halal authentication-a review”, Int. Food Res. J., Vol. 25, Pp. 1322–1331, 2018. Search in Google Scholar

[9] I. Mafra, I.M.P.L.V.O. Ferreira, and M.B.P.P. Oliveira, “Food authentication by PCR-based methods”, Eur. Food Res. and Technol., Vol. 227, Pp. 649–665, 2008. DOI: 10.1007/s00217-007-0782-x Open DOISearch in Google Scholar

[10] J.S. Amaral, G. Santos, M.B.P.P. Oliveira, and I. Mafra, “Quantitative detection of pork meat by EvaGreen real-time PCR to assess the authenticity of processed meat products”, Food Control., Vol. 72, Pp. 53–61, 2017. DOI: 10.1016/j.foodcont.2016.07.029 Open DOISearch in Google Scholar

[11] C.H. Shen, “Amplification of Nucleic Acids, in: C.-H. Shen (Ed.)”, Diagnostic Molecular Biology, Academic Press, UK. Pp. 215–247, 2019.10.1016/B978-0-12-802823-0.00009-2 Search in Google Scholar

[12] K. Kadri, “Polymerase Chain Reaction (PCR): Principle and Applications, in: M. L. Nagpal, O.-M. Boldura, C. Baltă and S. Enany (Eds.)”, Synthetic Biology - New Interdisciplinary Science, IntechOpen, UK. Pp. 1-17, 2020.10.5772/intechopen.86491 Search in Google Scholar

[13] Y. Gökmen-Polar, “Overview of PCR-Based Technologies and Multiplexed Gene Analysis for Biomarker Studies, in: S. Badve and G. Kumar (Eds.)”, Predictive Biomarkers in Oncology,.Springer Nature, Switzerland. Pp. 63–73, 2019.10.1007/978-3-319-95228-4_5 Search in Google Scholar

[14] M.A.M. Hossain, M.E. Ali, S. Sultana, Asing, S.Q. Bonny, M.A. Kader, and M.A. Rahman, “Quantitative Tetraplex Real-Time Polymerase Chain Reaction Assay with TaqMan Probes Discriminates Cattle, Buffalo, and Porcine Materials in Food Chain”, J. of Agri. and Food Chem., Vol. 65, Pp. 3975–3985, 2017. DOI: 10.1021/acs.jafc.7b0073028481513 Open DOISearch in Google Scholar

[15] E. Asing, S.B.A. Ali, M. Hamid, M.N.U. Hossain, S.M.A. Ahamad, N. Hossain, N. Naquiah, and I.S.M. Zaidul, “Duplex real-time PCR assay using SYBR Green to detect and quantify Malayan box turtle (Cuora amboinensis) materials in meatballs, burgers, frankfurters and traditional Chinese herbal jelly powder”, Food Addit. and Contam. - Part A Chem. Anal. Control Expo. and Risk Assess., Vol. 33, Pp. 1643–1659, 2016. DOI: 10.1080/19440049.2016.123640327643977 Open DOISearch in Google Scholar

[16] E. Navarro, G. Serrano-Heras, M.J. Castaño, and J. Solera, “Real-time PCR detection chemistry”, Clin. Chim. Acta., Vol. 439, Pp. 231–250, 2015. DOI: 10.1016/j.cca.2014.10.01725451956 Open DOISearch in Google Scholar

[17] W.M. Nelson, G.W. Long, and L.M. Cockrell, “Multiplexed, lateral flow, polymerase chain reaction (PCR) techniques for biological identification, in: R. P. Schaudies (Eds.)”, Biological Identification: DNA Amplification and Sequencing, Optical Sensing, Lab-On-Chip and Portable Systems, Elsevier Science and Technology, UK. Pp. 54–68, 2014.10.1533/9780857099167.1.54 Search in Google Scholar

[18] Life Technologies Corporation, Real-time PCR handbook, Pp. 1–68, 2015. Search in Google Scholar

[19] Primerdesign, Beginner’s Guide to Real-Time PCR Basic Principles, Pp. 1-7, 2018. Search in Google Scholar

[20] M. Kubista, J.M. Andrade, M. Bengtsson, A. Forootan, J. Jonák, K. Lind, R. Sindelka, R. Sjöback, B. Sjögreen, L. Strömbom, A. Ståhlberg, and N. Zoric, “The real-time polymerase chain reaction”, Mol. Aspects of Med., Vol. 27, Pp. 95–125, 2006. DOI: 10.1016/j.mam.2005.12.00716460794 Open DOISearch in Google Scholar

[21] Bio-Rad, Introduction to PCR Primer & Probe Chemistries, retrieved on 28 March 2021 at https://www.bio-rad.com/en-sg/applications-technologies/introduction-pcr-primer-probe-chemistries?ID=LUSOJW3Q3#1 Search in Google Scholar

[22] S.H. Han, H.S. Oh, and I.C. Cho, “Identifying the Species of Origin in Commercial Sausages in South Korea”, J. of Appl. Anim. Res., Vol. 45, Pp. 179–184, 2017. DOI: 10.1080/09712119.2015.1124334 Open DOISearch in Google Scholar

[23] M.A. Rachmadhani, Warisman, Suryani, and Desriani, “In-house validation and calibration of pork detection using duplex SYBR-Green I real-time PCR approach”, Int. Food Res. J., 26, Pp. 509–516, 2019. Search in Google Scholar

[24] Rahmawati, T.J. Sismindari, Raharjo, Sudjadi, and A. Rohman, “Analysis of pork contamination in Abon using mitochondrial D-Loop22 primers using real time polymerase chain reaction method”, Int. Food Res. J., Vol. 23, Pp. 370–374, 2016. Search in Google Scholar

[25] T.J. Raharjo, E.N. Alfiraza, E. Enjelina, and D. Pranowo, “Validation of a Non-Specific Dye Real-Time PCR Assay for Porcine Adulteration in Meatball Using ND5 Primer, Indones”, J. of Chem., Vol. 17, Pp. 167–174, 2017. DOI: 10.22146/ijc.22646 Open DOISearch in Google Scholar

[26] H. Lubis, N.T. Salihah, N.A. Norizan, M.M. Hossain, and M.U. Ahmed, “Fast and sensitive real-time PCR-based detection of porcine DNA in food samples by using EvaGreen Dye”, Food Sci. and Technol. Res., Vol. 24, Pp. 803–810, 2018. DOI: 10.3136/fstr.24.803 Open DOISearch in Google Scholar

[27] Q. Zia, M. Alawami, N.F.K. Mokhtar, R.M.H.R. Nhari, and I. Hanish, “Current analytical methods for porcine identification in meat and meat products”, Food Chem., Vol. 324, Pp. 126664, 2020. DOI: 10.1016/j.foodchem.2020.12666432380410 Open DOISearch in Google Scholar

[28] N.A. Mohamad, A.F. El Sheikha, S. Mustafa, and N.F.K. Mokhtar, “Comparison of gene nature used in real-time PCR for porcine identification and quantification: A review”, Food Res. Int., Vol. 50, Pp. 330–338, 2013. DOI: 10.1016/j.foodres.2012.10.047 Open DOISearch in Google Scholar

[29] M.E. Ali, U. Hashim, S. Mustafa, Y.B. Che Man, T.S. Dhahi, M. Kashif, M.K. Uddin, and S.B. Abd Hamid, “Analysis of pork adulteration in commercial meatballs targeting porcine-specific mitochondrial cytochrome b gene by TaqMan probe real-time polymerase chain reaction”, Meat Sci., Vol. 91, Pp. 454–459, 2012. DOI: 10.1016/j.meatsci.2012.02.03122444666 Open DOISearch in Google Scholar

[30] N.A.M. Al-Rashedi, and E.U. Hateem, “Detection of Pork in Canned Meat using TaqMan Real-time PCR”, Muthanna J. of Pure Sci., Vol. 3, Pp. 16-20, 2016. Search in Google Scholar

[31] T.J. Raharjo, Y.N.C. Chudori, and F.W. Agustina, “TaqMan probe real-time polymerase chain reaction targeting the ATPase 6 gene for the detection of pork adulteration in meat and meatballs”, J. of Food Saf., Vol. 39, Pp. 1–6, 2019. DOI:org/10.1111/jfs.12715 Open DOISearch in Google Scholar

[32] M.H.M. Yusop, S. Mustafa, Y.B.C. Man, A.R. Omar, and N.F.K. Mokhtar, “Detection of Raw Pork Targeting Porcine-Specific Mitochondrial Cytochrome B Gene by Molecular Beacon Probe Real-Time Polymerase Chain Reaction”, Food Anal. Methods., Vol. 5, Pp. 422–429, 2012. DOI: 10.1007/s12161-011-9260-y Open DOISearch in Google Scholar

[33] X. Cheng, W. He, F. Huang, M. Huang, and G. Zhou, “Multiplex real-time PCR for the identification and quantification of DNA from duck, pig and chicken in Chinese blood curds”, Food Res. Int., Vol. 60, Pp. 30-37, 2014. DOI: 10.1016/j.foodres.2014.01.047 Open DOISearch in Google Scholar

[34] R. Köppel, J. Ruf, F. Zimmerli, and A. Breitenmoser, “Multiplex Real-Time PCR for the Detection and Quantification of DNA from Beef, Pork, Chicken and Turkey”, Eur. Food Res. Technol., Vol. 227, Pp. 1199–1203, 2008. DOI:10.1007/s00217-008-0837-7 Open DOISearch in Google Scholar

[35] D. Svec, A. Tichopad, V. Novosadova, M.W. Pfaffl, and M. Kubista, “How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments”, Biomol. Detect. and Quantif., Vol. 3, Pp. 9–16, 2015. DOI: 10.1016/j.bdq.2015.01.005482221627077029 Open DOISearch in Google Scholar

[36] S. Taylor, M. Wakem, G. Dijkman, M. Alsarraj, and M. Nguyen, “A practical approach to RT-qPCR-Publishing data that conform to the MIQE guidelines”, Methods, Vol. 50, Pp. S1-S5, 2010. DOI: 10.1016/j.ymeth.2010.01.00520215014 Open DOISearch in Google Scholar

[37] BioRad, Reagent Comparison Guide for Real-Time PCR, BioRad Bulletin 6252. 2013. Search in Google Scholar

[38] Z. Kesmen, A.E. Yetiman, F. Şahin, and H. Yetim, “Detection of Chicken and Turkey Meat in Meat Mixtures by Using Real-Time PCR Assays”, J. of Food Sci., Vol. 77, Pp. 167–173, 2012. DOI: 10.1111/j.1750-3841.2011. 02536.x Open DOISearch in Google Scholar

[39] A. Iwobi, D. Sebah, I. Kraemer, C. Losher, G. Fischer, U. Busch, and I. Huber, “A Multiplex Real-Time PCR Method for the Quantification of Beef and Pork Fractions in Minced Meat”, Food Chem., 169, Pp. 305–313, 2015. DOI: 10.1016/j.foodchem.2014.07.13925236231 Open DOISearch in Google Scholar