<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Biomedical spectroscopy has gained attention in the past few years for disease diagnosis. Fluorescence and Raman spectroscopies provide finger-print information related to biochemical and morphological alterations when tissues progress from the normal to a malignant stage. Usually, freshly excised tissue specimens are preferred for bio-spectroscopic studies. However, ethical issues, sample availability and distance between the surgery room and the laboratory provide an impelling restriction for <italic>in-vitro</italic> spectroscopic studies using freshly excised samples. After surgical resection tissues are fixed in 4% formalin for histological studies under a light microscope. The process of fixation prevents degradation of tissues. In this study, we probe the use of formalin fixed sample for differentiating normal and dysplastic brain tissues using fluorescence and Raman spectroscopies. It was found that fluorescence spectral profile changes in the wavelength range from 550-750 nm between dysplastic and tumor samples. Also, significant differences were found in the Raman spectral profiles of such samples. The results indicate a potential diagnostic application of spectroscopy in formalin fixed brain samples for differentiating dysplastic and tumor brain tissues.</p></abstract>

Biomedical spectroscopy has gained attention in the past few years for disease diagnosis. Fluorescence and Raman spectroscopies provide finger-print information related to biochemical and morphological alterations when tissues progress from the normal to a malignant stage. Usually, freshly excised tissue specimens are preferred for bio-spectroscopic studies. However, ethical issues, sample availability and distance between the surgery room and the laboratory provide an impelling restriction for in-vitro spectroscopic studies using freshly excised samples. After surgical resection tissues are fixed in 4% formalin for histological studies under a light microscope. The process of fixation prevents degradation of tissues. In this study, we probe the use of formalin fixed sample for differentiating normal and dysplastic brain tissues using fluorescence and Raman spectroscopies. It was found that fluorescence spectral profile changes in the wavelength range from 550-750 nm between dysplastic and tumor samples. Also, significant differences were found in the Raman spectral profiles of such samples. The results indicate a potential diagnostic application of spectroscopy in formalin fixed brain samples for differentiating dysplastic and tumor brain tissues.

Bimodal Spectroscopy of Formalin Fixed Samples to Discriminate Dysplastic and Tumor Brain Tissues

European Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Via Nello Carrara, 1 - 50019, Sesto Fiorentino, ITALY

National Institute of Optics, National Research Council (INO-CNR), Largo Enrico Fermi 6 - 50125, Florence, ITALY

Division of Neurosurgery, Department of Neuroscience I, “Anna Meyer” Pediatric Hospital, Viale Gaetano Pieraccini 24 – 50141, Florence, ITALY

Division of Pathology, Department of Critical Care Medicine and Surgery, University of Florence, Viale Giovanni Battista Morgagni 85 - 50134, Florence, ITALY

Department of Physics, University of Florence, Via Giovanni Sansone 1 - 50019, Sesto Fiorentino, ITALY

Latvian Journal of Physics and Technical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

FORMALĪNĀ FIKSĒTU SMADZEŅU AUDU PARAUGU BIMODĀLĀ SPETROSKOPIJA DISPLASTISKU UN AUDZĒJA AUDU ATŠĶIRŠANAI

Biomedical spectroscopy has gained attention in the past few years for disease diagnosis. Fluorescence and Raman spectroscopies provide finger-print...

Bimodal Spectroscopy of Formalin Fixed Samples to Discriminate Dysplastic and Tumor Brain Tissues

Publicado en línea: 15 dic 2014

Páginas: 14 - 20

DOI: https://doi.org/10.2478/lpts-2014-0027

Palabras clave
formalin fixation, fluorescence, Raman spectroscopy, brain tumor, dysplasia

© 2014 S. Anand et. al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Bimodal Spectroscopy of Formalin Fixed Samples to Discriminate Dysplastic and Tumor Brain Tissues

Publicado en línea: 15 dic 2014

Páginas: 14 - 20

DOI: https://doi.org/10.2478/lpts-2014-0027

Palabras claveformalin fixation, fluorescence, Raman spectroscopy, brain tumor, dysplasia

© 2014 S. Anand et. al.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
formalin fixation, fluorescence, Raman spectroscopy, brain tumor, dysplasia