Application of a liposomal subunit vaccine in chickens for reduction of Campylobacter gut colonisation

Campylobacter are the most common cause of food poisoning, which manifests itself in diarrhoea of varying severity. Additionally, because of the increasing number of people with immune deficiencies, more frequent serious complications of Campylobacter infections are being observed. The main source of infection is the consumption of contaminated poultry meat, which is a consequence of the insufficiency of current hygiene and biosecurity to control Campylobacter or eliminate it from the poultry food chain.

Material and Methods

Two hybrid proteins, presenting selected epitopes of the Campylobacter antigens CjaD and EF-Tu, were developed based on the highly immunogenic proteins CjaA and CjaC. Four groups of chickens were vaccinated with different preparations (a mixture of both hybrid proteins encapsulated in anionic or neutral liposomes) and different doses (a single dose given on the day of hatching or two doses given on days 1 and 14 of life). The number of Campylobacter was assessed in the intestinal contents of vaccinated birds.

Results

No statistically significant differences in colonisation levels were observed between chickens immunised with neutral liposomes containing hybrid proteins and their non-immunised counterparts, regardless of dosage regimen.

Conclusion

Although immunisation of chickens did not produce the expected results, the approach used has great potential, which is worth further investigation and development.

Idioma:: Inglés

Calendario de la edición:: 4 veces al año
Temas de la revista:: Ciencias de la vida, Biología molecular, Microbiología y virología, Ciencias de la vida, otros, Medicina, Medicina veterinaria

RSS Feed de revista

Application of a liposomal subunit vaccine in chickens for reduction of Campylobacter gut colonisation

Anna Łasica

Renata Godlewska

Jerzy Gubernator

Anna Jakubiak-Augustyn

Paweł Majewski

Agnieszka Wyszyńska

Publicado en línea: 06 nov 2024

Páginas: 487 - 496

Recibido: 02 jun 2024

Aceptado: 28 oct 2024

DOI: https://doi.org/10.2478/jvetres-2024-0062

Palabras clavevaccine, liposomes, hybrid proteins

© 2024 Anna Łasica et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Material and Methods

Results

Conclusion

Palabras clave
vaccine, liposomes, hybrid proteins