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The serological and genetic diversity of the Leptospira interrogans Icterohaemorrhagiae serogroup circulating in the UK

Zbigniew Arent
Zbigniew Arent
, 
Colm Gilmore
Colm Gilmore
, 
Laura Pardyak
Laura Pardyak
, 
Klaudia Dubniewicz
Klaudia Dubniewicz
, 
Barry McInerney
Barry McInerney
 y 
William Ellis
William Ellis
   | 19 dic 2023
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Publicado en línea: 19 dic 2023
Páginas: 529 - 536
Recibido: 12 jun 2023
Aceptado: 06 nov 2023
DOI: https://doi.org/10.2478/jvetres-2023-0063
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© 2023 Zbigniew Arent et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Restriction endonuclease analysis patterns of chromosomal DNA from Leptospira strains digested with EcoRI. Lanes: 1 – Icterohaemorrhagiae (RGA strain); 2 – Copenhageni (M20 strain); 3 – Monymusk (LT 75-68 strain); 4 – Budapest (PV-1 strain); 5 – Mankarso (Mankarso strain); 6 – Naam (Naam strain); 7 – Birkini (Birkin strain); 8 – Bogvere (LT 60-69 strain); 9 – Dakota (Grand River strain); 10 – Gem (Simon strain); 11 – Lai (Lai strain); 12 – Mwogolo (Mwogolo strain); 13 – Ndambari (Ndambari strain); 14 – Ndahambukuje (Ndahambukuje strain); 15 – Smithi (Smith strain); 16 – Tonkini (LT 96-68 strain)
Restriction endonuclease analysis patterns of chromosomal DNA from Leptospira strains digested with EcoRI. Lanes: 1 – Icterohaemorrhagiae (RGA strain); 2 – Copenhageni (M20 strain); 3 – Monymusk (LT 75-68 strain); 4 – Budapest (PV-1 strain); 5 – Mankarso (Mankarso strain); 6 – Naam (Naam strain); 7 – Birkini (Birkin strain); 8 – Bogvere (LT 60-69 strain); 9 – Dakota (Grand River strain); 10 – Gem (Simon strain); 11 – Lai (Lai strain); 12 – Mwogolo (Mwogolo strain); 13 – Ndambari (Ndambari strain); 14 – Ndahambukuje (Ndahambukuje strain); 15 – Smithi (Smith strain); 16 – Tonkini (LT 96-68 strain)

Fig. 2.

Unweighted pair-group method with arithmetic mean (UPGMA) tree based on the 12 variable-number tandem-repeat (VNTR) loci (V4, V7, V9, V10, V11, V19, V27, V29, V30, V31, V36 and V50). As only a partial repeat was present in V23 for all studied isolates, this locus was omitted in the UPGMA cluster analysis. Different VNTR profiles of 19 isolates revealed 5 distinct genotypes (A to E)
Unweighted pair-group method with arithmetic mean (UPGMA) tree based on the 12 variable-number tandem-repeat (VNTR) loci (V4, V7, V9, V10, V11, V19, V27, V29, V30, V31, V36 and V50). As only a partial repeat was present in V23 for all studied isolates, this locus was omitted in the UPGMA cluster analysis. Different VNTR profiles of 19 isolates revealed 5 distinct genotypes (A to E)

Reciprocals of agglutination titres of a panel of three monoclonal antibodies with reference strains of the Copenhageni (M20) and Icterohaemorrhagiae (RGA) serovars of Leptospira interrogans and two field strains of the bacteria. The rest of the field strains gave the same results as those two representatives of each serovar

Serovar Strain Monoclonal antibody
F12C3 F70C14 F70C24
Copenhageni M20 10,000 - 10,000
Copenhageni AB102 10,000 10,000
Icterohaemorrhagiae RGA 30,000 10,000 -
Icterohaemorrhagiae X240 30,000 10,000 -

Source and origin characteristics of the Icterohaemorrhagiae serogroup Leptospira interrogans isolates used in this study

Strain identifier Host Tissue of origin Clinical symptoms Serovar GenBank accession No.
23112 Human Blood Anorexia, fever Copenhageni
S72 Horse Foetal kidney Abortion Copenhageni GCA_017653945.1
X240 Dog Kidney Anorexia, fever, prostration, death Icterohaemorrhagiae GCA_017653925.1
R287 Cow Foetal kidney Abortion Copenhageni GCA_017653725.1
AB102 Cow Foetal kidney Abortion Copenhageni
S606 Cow Foetal lung Abortion Copenhageni GCA_017653855.1
W26/2 Hedgehog Kidney Asymptomatic Copenhageni GCA_017653845.1
B33 Badger Kidney Asymptomatic Copenhageni GCA_017653825.1
B42 Badger Kidney Asymptomatic Copenhageni GCA_017653785.1
B126 Badger Kidney Asymptomatic Icterohaemorrhagiae GCA_017654005.1
B167 Badger Kidney Asymptomatic Copenhageni GCA_017653985.1
S1006 Badger Kidney Asymptomatic Copenhageni GCA_017654105.1
S199/2 Brown rat Kidney Asymptomatic Icterohaemorrhagiae GCA_017653805.1
S199/3 Brown rat Kidney Asymptomatic Copenhageni GCA_017654025.1
S199/4 Brown rat Kidney Asymptomatic Copenhageni GCA_017653755.1
S199/5 Brown rat Kidney Asymptomatic Copenhageni GCA_017654055.1
S199/6 Brown rat Kidney Asymptomatic Copenhageni GCA_017653965.1
S199/7 Brown rat Kidney Asymptomatic Copenhageni
S199/12 Brown rat Kidney Asymptomatic Copenhageni GCA_017654085.1

List of the Icterohaemorrhagiae serogroup Leptospira reference strains used in the restriction endonuclease analysis

Species Serogroup Serovar Strain Country Source
L. interrogans Icterohaemorrhagiae Icterohaemorrhagiae RGA Belgium Human
L. interrogans Icterohaemorrhagiae Copenhageni M20 Denmark Human
L. interrogans Icterohaemorrhagiae Monymusk LT 75-68 Jamaica Rat
L. interrogans Icterohaemorrhagiae Budapest PV-1 Hungary Laboratory rat
L. interrogans Icterohaemorrhagiae Mankarso Mankarso Indonesia Human
L. interrogans Icterohaemorrhagiae Naam Naam Indonesia Human
L. interrogans Icterohaemorrhagiae Birkini Birkin Malaysia Human
L. kirschneri Icterohaemorrhagiae Bogvere LT 60-69 Jamaica Rat
L. kirschneri Icterohaemorrhagiae Dakota Grand River USA Water
L. interrogans Icterohaemorrhagiae Gem Simon Sri Lanka Human
L. interrogans Icterohaemorrhagiae Lai Lai China Human
L. kirschneri Icterohaemorrhagiae Mwogolo Mwogolo Zaire Human
L. kirschneri Icterohaemorrhagiae Ndambari Ndambari Zaire Human
L. kirschneri Icterohaemorrhagiae Ndahambukuje Ndahambukuje Zaire Human
L. interrogans Icterohaemorrhagiae Smithi Smith Malaysia Human
L. borgpetersenii Icterohaemorrhagiae Tonkini LT 96-68 Vietnam Human
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