Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats

Ashraf M. Morgan; Hanan A. Ogaly; Shaimaa Kamel; Maha M. Rashad; Eman I. Hassanen; Marwa A. Ibrahim; Mona K. Galal; Aya M. Yassin; Sharah A. Al Dulmani; Fatimah A.M. Al-Zahrani; Ahmed M. Hussien

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Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats

Journal of Veterinary Research

Volumen 67 (2023): Edición 3 (September 2023)

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Publicado en línea: 20 sept 2023

Páginas: 459 - 469

Recibido: 24 dic 2022

Aceptado: 26 jun 2023

DOI: https://doi.org/10.2478/jvetres-2023-0039

Palabras clave
penconazole, Nrf2, HO-1, Keap1, NF-κB

© 2023 Ashraf M. Morgan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effects of NAC on (A) malondialdehyde and (B) TAC in the liver and kidneys of adult male albino Wistar PEN-exposed rats. Data are presented as mean ± standard error of the mean (whiskers) (n = 6)
# – significantly different to NC group; * – significantly different to PEN group; NC – normal control; PEN – penconazole; NAC – N-acetyl-1-cysteine; TAC – total antioxidant capacity — Effects of NAC on (A) malondialdehyde and (B) TAC in the liver and kidneys of adult male albino Wistar PEN-exposed rats. Data are presented as mean ± standard error of the mean (whiskers) (n = 6) # – significantly different to NC group; * – significantly different to PEN group; NC – normal control; PEN – penconazole; NAC – N-acetyl-1-cysteine; TAC – total antioxidant capacity

Effect of N-acetyl-1-cysteine (NAC) in adult male albino Wistar rats against penconazole (PEN)-induced alterations on hepatic messenger RNA expression of nuclear factor erythroid 2-related factor 2 (Nrf2) (A), Kelch-like erythroid cell-derived protein 1 (Keap1) (B) and haem oxygenase 1 (HO-1) (C). Data are presented as mean ± standard error of the mean (whiskers) (n = 6)
GAPDH – glyceraldehyde 3-phosphate dehydrogenase; # – significantly different to normal control (NC) group; * – significantly different to PEN group — Effect of N-acetyl-1-cysteine (NAC) in adult male albino Wistar rats against penconazole (PEN)-induced alterations on hepatic messenger RNA expression of nuclear factor erythroid 2-related factor 2 (Nrf2) (A), Kelch-like erythroid cell-derived protein 1 (Keap1) (B) and haem oxygenase 1 (HO-1) (C). Data are presented as mean ± standard error of the mean (whiskers) (n = 6) GAPDH – glyceraldehyde 3-phosphate dehydrogenase; # – significantly different to normal control (NC) group; * – significantly different to PEN group

Effect of N-acetyl-1-cysteine (NAC) in adult male albino Wistar rats against penconazole (PEN)-induced alterations on renal mRNA expression of nuclear factor erythroid 2-related factor 2 (Nrf2) (A), Kelch-like erythroid cell-derived protein 1 (Keap1) (B) and haem oxygenase 1 (HO-1) (C). Data are presented as mean ± standard error of the mean (whiskers) (n = 6)
GAPDH – glyceraldehyde 3-phosphate dehydrogenase; # – significantly different to normal control (NC) group; * – significantly different to PEN group — Effect of N-acetyl-1-cysteine (NAC) in adult male albino Wistar rats against penconazole (PEN)-induced alterations on renal mRNA expression of nuclear factor erythroid 2-related factor 2 (Nrf2) (A), Kelch-like erythroid cell-derived protein 1 (Keap1) (B) and haem oxygenase 1 (HO-1) (C). Data are presented as mean ± standard error of the mean (whiskers) (n = 6) GAPDH – glyceraldehyde 3-phosphate dehydrogenase; # – significantly different to normal control (NC) group; * – significantly different to PEN group

Photomicrograph of adult male albino Wistar rat liver tissue sections stained with haematoxylin and eosin representing the control group with normal histological structure (A), the penconazole (PEN)-receiving group showing hepatocellular vacuolisation (black arrows), necrosis (blue arrows), atrophy (black triangles), macrovesicular steatosis (red arrows), haemorrhaging (red star) and inflammatory cells infiltration (black stars) (B–F) and the PEN+N-acetyl-1-cysteine–receiving group showing sparse cell necrosis (blue arrows) and slight portal inflammation (black star) (G and H)

Photomicrograph of adult male albino Wistar rat kidney tissue sections stained with haematoxylin and eosin representing the control group with normal histological structure (A), the penconazole (PEN)-receiving group showing cytoplasmic vacuolisation (black arrows), necrosis (blue arrows) in the renal epithelium, intraluminal hyaline cast and droplets (black triangles), vascular congestion and haemorrhaging (red stars), inflammatory cells infiltration (black stars), capsular fibrosis (blue star) and glomerular degeneration (black circles) (B–E) and the PEN+N-acetyl-1-cysteine–receiving group showing mild degenerative changes in the tubular epithelium and some glomeruli (black arrows) (F)

Photomicrograph of adult male albino Wistar rat liver tissue sections stained by immunoperoxidase to localise TNF-α and NF-κB. The control group with normal TNF-α and NF-κB cytoplasmic expression (A and B), PEN group showing strong positive expression of both immune markers (C and D) and the PEN+NAC–receiving group showing weak positive expression of TNF-α and NF-κB (E and F)

Photomicrograph of adult male albino Wistar rat kidney tissue sections stained by immunoperoxidase to localise TNF-α and NF-κB. The control group with normal TNF-α and NF-κB cytoplasmic expression (A and B), PEN group showing strong positive expression of both immune markers (C and D) and the PEN+NAC–receiving group showing weak positive expression of TNF-α and NF-κB (E and F)

The microscopic lesion scoring in adult male albino Wistar rats unexposed to penconazole (PEN), exposed to it, and exposed to it with N-acetyl-1 cysteine (NAC) coadministration

	NC	NAC	PEN	PEN+NAC
Hepatic lesion scoring
HCD	0 ^a	1 ^b	4 ^c	2 ^d
HCN	0 ^a	1 ^b	2 ^c	1 ^b
ICB	0 ^a	0 ^a	4 ^b	1 ^c
Congestion	0 ^a	1 ^b	3 ^c	1 ^b
Haemorrhage	0 ^a	0 ^a	2 ^b	0 ^a
Inflammation	0 ^a	0 ^a	2 ^b	1 ^c
Renal lesion scoring
RTD	0 ^a	1 ^b	4 ^c	2 ^d
RTN	0 ^a	1 ^b	4 ^c	2 ^d
ICB	0 ^a	0 ^a	4 ^b	1 ^c
Congestion	0 ^a	1 ^b	4 ^c	2 ^d
Haemorrhage	0 ^a	0 ^a	2 ^b	0 ^a
Inflammation	0 ^a	0 ^a	4 ^b	1 ^c

Effects of N-acetyl-1-cysteine (NAC) on liver and kidney function tests in adult male albino Wistar rats exposed to penconazole (PEN)

Group	AST (U/L)	ALT (U/L)	Creatinine (mg/dL)	Urea (mg/dL)
NC	69.66 ± 1.16^#	24.70 ± 0.82^#	0.72 ± 0.01^#	25.18 ± 0.56
PEN	114.05 ± 4.79^*	51.35 ± 1.46^*	1.62 ± 0.02^*	57.36 ±0.60^*
PEN+NAC	86.20 ± 2.15^*^#	38.24 ± 1.55^*^#	0.85 ± 0.01^#	38.51 ± 0.45^*^#
NAC	71.23 ± 1.52^#	27.12 ± 0.67^#	0.68 ± 0.01^#	27.94 ± 0.35^#

eISSN:: 2450-8608
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Calendario de la edición:: 4 veces al año
Temas de la revista:: Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine

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Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats

Publicado en línea: 20 sept 2023

Páginas: 459 - 469

Recibido: 24 dic 2022

Aceptado: 26 jun 2023

DOI: https://doi.org/10.2478/jvetres-2023-0039

Palabras clave
penconazole, Nrf2, HO-1, Keap1, NF-κB

© 2023 Ashraf M. Morgan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Fig. 6.

Fig. 7.

The microscopic lesion scoring in adult male albino Wistar rats unexposed to penconazole (PEN), exposed to it, and exposed to it with N-acetyl-1 cysteine (NAC) coadministration

Effects of N-acetyl-1-cysteine (NAC) on liver and kidney function tests in adult male albino Wistar rats exposed to penconazole (PEN)

Protective effects of N-acetyl-l-cysteine against penconazole-triggered hepatorenal toxicity in adult rats

Publicado en línea: 20 sept 2023

Páginas: 459 - 469

Recibido: 24 dic 2022

Aceptado: 26 jun 2023

DOI: https://doi.org/10.2478/jvetres-2023-0039

Palabras clavepenconazole, Nrf2, HO-1, Keap1, NF-κB

© 2023 Ashraf M. Morgan et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Fig. 6.

Fig. 7.

The microscopic lesion scoring in adult male albino Wistar rats unexposed to penconazole (PEN), exposed to it, and exposed to it with N-acetyl-1 cysteine (NAC) coadministration

Effects of N-acetyl-1-cysteine (NAC) on liver and kidney function tests in adult male albino Wistar rats exposed to penconazole (PEN)

Palabras clave
penconazole, Nrf2, HO-1, Keap1, NF-κB