Acceso abierto

Genotoxic effect of cadmium and zinc in the peripheral erythrocytes of Prussian carp (Carassius gibelio B.)

Ewa Drąg-Kozak

Ewa Drąg-Kozak

Department of Animal Nutrition and Biotechnology, and FisheriesKraków, Poland
Buscar este autor en
Sciendo|Google Scholar
Drąg-Kozak, Ewa
, 
Marta Kuchta-Gładysz

Marta Kuchta-Gładysz

Department of Animal Reproduction, Anatomy and GenomicsKraków, Poland
Buscar este autor en
Sciendo|Google Scholar
Kuchta-Gładysz, Marta
, 
Anna Grzesiakowska

Anna Grzesiakowska

Department of Animal Reproduction, Anatomy and GenomicsKraków, Poland
Buscar este autor en
Sciendo|Google Scholar
Grzesiakowska, Anna
, 
Ewa Łuszczek-Trojnar

Ewa Łuszczek-Trojnar

Department of Animal Nutrition and Biotechnology, and FisheriesKraków, Poland
Buscar este autor en
Sciendo|Google Scholar
Łuszczek-Trojnar, Ewa
 y 
Magdalena Socha

Magdalena Socha

Department of Animal Physiology and Endocrinology, University of Agriculture in KrakowKraków, Poland
Buscar este autor en
Sciendo|Google Scholar
Socha, Magdalena
  
04 nov 2022
Genotoxic effect of cadmium and zinc in the peripheral erythrocytes of Prussian carp (Carassius gibelio B.)'s Cover Image
Acerca de este artículo
Artículo anterior
Artículo siguiente
Cite
Descargar portada
Publicado en línea: 04 nov 2022
Páginas: 619 - 628
Recibido: 11 abr 2022
Aceptado: 12 oct 2022
DOI: https://doi.org/10.2478/jvetres-2022-0057
Palabras clave
© 2022 E. Drąg-Kozak et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Erythrocytes of Prussian carp with different levels of DNA damage in the comet assay: cells from control samples after 14 days (a), after 21 days (c), and after 28 days (e); cells after exposure to Cd for 14 days (b), for 21 days (d) and for 28 days (f). 400×
Erythrocytes of Prussian carp with different levels of DNA damage in the comet assay: cells from control samples after 14 days (a), after 21 days (c), and after 28 days (e); cells after exposure to Cd for 14 days (b), for 21 days (d) and for 28 days (f). 400×

Fig. 2

Giemsa-stained peripheral blood smear of erythrocytes of Prussian carp with micronuclei (a and b), a nuclear bud (c), a notched nucleus (d), binucleated cells with unequal nuclear division (e), and a cell with abnormal cytoplasm (f). Arrows show the abnormalities. Erythrocytes are from the control group after 21 days of the experiment (c and d), exposed to Cd after 14 days (f) and exposed to Cd after 28 days (a, b and e). 1000×
Giemsa-stained peripheral blood smear of erythrocytes of Prussian carp with micronuclei (a and b), a nuclear bud (c), a notched nucleus (d), binucleated cells with unequal nuclear division (e), and a cell with abnormal cytoplasm (f). Arrows show the abnormalities. Erythrocytes are from the control group after 21 days of the experiment (c and d), exposed to Cd after 14 days (f) and exposed to Cd after 28 days (a, b and e). 1000×

Comet assay results for Prussian carp exposed to different treatments of cadmium, zinc and their binary mixture

Group
Control Cd Zn Cd + Zn
Day DNA in Tail (%)
14 26.30 ± 0.56 Aa 31.78 ± 0.69 Ab 33.35 ± 0.67 Ab 37.87 ± 0.76 Ac
21 28.08 ± 0.65 Aa 31.84 ± 0.59 Ab 24.40 ± 0.53 Bc 31.89 ± 0.64 Bb
28 32.04 ± 0.61 Ba 32.87 ± 0.67 Aa 28.58 ± 0.51 Cb 31.90 ± 0.59 Ba

The frequency of erythrocyte nuclear and cellular abnormalities in Prussian carp exposed to different concentrations of cadmium, zinc and their binary mixture

Group
Control Cd Zn Cd + Zn
Day MN (one or more micronuclei)
14 2.30 ± 0.36 Aa 12.49 ± 1.08 Ab 7.35 ± 0.80 Ac 16.07 ± 1.68 Ab
21 1.29 ± 0.21 Ba 4.00 ± 0.48 Bb 4.61 ± 1.14 ABb 3.67 ± 0.43 Bb
28 3.11 ± 0.34 Aab 2.33 ± 0.298 Ca 3.69 ± 0.34 Bbc 4.53 ± 0.63 Bc
Idioma:
Inglés
Calendario de la edición:
4 veces al año
Temas de la revista:
Ciencias de la vida, Biología molecular, Microbiología y virología, Ciencias de la vida, otros, Medicina, Medicina veterinaria
RSS Feed de revista