<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2022-0016_s_005">
<h3>Introduction</h3>
<p>African swine fever virus (ASFV) causes one of the most dangerous diseases of pigs and wild boar – African swine fever (ASF). Since its second introduction into Europe (in 2007), the disease has been spreading consistently, and now ASF-free European countries are at risk. Complex interactions between the host’s immune system and the virus have long prevented the development of a safe vaccine against ASF. This study analysed the possibility of neutralisation of the ASFV <italic>in vitro</italic> by sera collected from ASF-survivor animals.</p></sec>
<sec id="j_jvetres-2022-0016_s_006">
<h3>Material and Methods</h3>
<p>Two pig and three wild boar serum samples were collected from previously selected potential ASF survivors. All sera presented high antibody titres (>5 log<sub>10</sub>/mL). Primary alveolar macrophages were cultured in growth medium containing 10% and 20% concentrations of selected sera and infected with a haemadsorbing ASFV strain (Pol18_28298_O111, genotype II). The progress of infection was investigated under a light microscope by observing the cytopathic effect (CPE) and the haemadsorption phenomenon. Growth kinetics were investigated using a real-time PCR assay.</p></sec>
<sec id="j_jvetres-2022-0016_s_007">
<h3>Results</h3>
<p>Haemadsorption inhibition was detected in the presence of almost all selected sera; however, the inhibition of virus replication <italic>in vitro</italic> was excluded. In all samples, a CPE and decreasing quantification cycle values of the viral DNA were found.</p></sec>
<sec id="j_jvetres-2022-0016_s_008">
<h3>Conclusion</h3>
<p>Anti-ASFV antibodies alone are not able to inhibit virus replication. Interactions between the humoral and cellular immune response which effectively combat the disease are implicated in an ASF-survivor’s organism.</p></sec></abstract>

Introduction
African swine fever virus (ASFV) causes one of the most dangerous diseases of pigs and wild boar – African swine fever (ASF). Since its second introduction into Europe (in 2007), the disease has been spreading consistently, and now ASF-free European countries are at risk. Complex interactions between the host’s immune system and the virus have long prevented the development of a safe vaccine against ASF. This study analysed the possibility of neutralisation of the ASFV in vitro by sera collected from ASF-survivor animals.

Material and Methods
Two pig and three wild boar serum samples were collected from previously selected potential ASF survivors. All sera presented high antibody titres (>5 log10/mL). Primary alveolar macrophages were cultured in growth medium containing 10% and 20% concentrations of selected sera and infected with a haemadsorbing ASFV strain (Pol18_28298_O111, genotype II). The progress of infection was investigated under a light microscope by observing the cytopathic effect (CPE) and the haemadsorption phenomenon. Growth kinetics were investigated using a real-time PCR assay.

Results
Haemadsorption inhibition was detected in the presence of almost all selected sera; however, the inhibition of virus replication in vitro was excluded. In all samples, a CPE and decreasing quantification cycle values of the viral DNA were found.

Conclusion
Anti-ASFV antibodies alone are not able to inhibit virus replication. Interactions between the humoral and cellular immune response which effectively combat the disease are implicated in an ASF-survivor’s organism.

ASF -survivors’ sera do not inhibit African swine fever virus replication <italic>in vitro</italic>

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

ASF -survivors’ sera do not inhibit African swine fever virus replication 

Introduction
African swine fever virus (ASFV) causes one of the most dangerous diseases of pigs and wild boar – African swine fever (ASF). Since its second...

ASF -survivors’ sera do not inhibit African swine fever virus replication in vitro

Publicado en línea: 25 mar 2022

Páginas: 21 - 27

Recibido: 22 dic 2021

Aceptado: 08 mar 2022

DOI: https://doi.org/10.2478/jvetres-2022-0016

Palabras clave
African swine fever, antibodies, ASFV, immune response, survivors

© 2022 M. Walczak et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

ASF -survivors’ sera do not inhibit African swine fever virus replication in vitro

Publicado en línea: 25 mar 2022

Páginas: 21 - 27

Recibido: 22 dic 2021

Aceptado: 08 mar 2022

DOI: https://doi.org/10.2478/jvetres-2022-0016

Palabras claveAfrican swine fever, antibodies, ASFV, immune response, survivors

© 2022 M. Walczak et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
African swine fever, antibodies, ASFV, immune response, survivors