<abstract xmlns="http://www.w3.org/1999/xhtml">
<sec id="j_jvetres-2021-0049_s_005">
<h3>Introduction</h3>
<p>Milk has been suggested to be a possible source of oestrogenically active compounds. In order to assess the health risk for milk consumers and ensure the safety of this staple part of the human diet, it is important to study the effect of xenooestrogen mixtures present in milk. This investigation used the available <italic>in vivo</italic> model to learn to what extent such compounds may be endocrine disruptors.</p></sec>
<sec id="j_jvetres-2021-0049_s_006">
<h3>Material and Methods</h3>
<p>The recommended immature golden hamster uterotrophic bioassay was chosen. A total of 132 animals were divided into nine groups of experimental animals and positive and negative control groups, each of 12 animals. The experimental females received <italic>ad libitum</italic> either one of five samples of raw cow’s milk from individual animals or one of four samples of pasteurised or ultra-high temperature treated cow’s milk as retail products. After 7 days, the animals were sacrificed and necropsied. Uterine weight increases were measured as the endpoint of oestrogenic activity in milk.</p></sec>
<sec id="j_jvetres-2021-0049_s_007">
<h3>Results</h3>
<p>The milk samples from individual cows and the retail milk samples did not show oestrogenic activity. However, in three groups, decreased uterine weights were observed.</p></sec>
<sec id="j_jvetres-2021-0049_s_008">
<h3>Conclusion</h3>
<p>Considering that milk supplies are beneficial to health, contamination in this food should be avoided. There is a need for further animal experiments and epidemiological studies are warranted to evaluate any causative role of milk in human endocrinological disorders.</p></sec>
</abstract>

Introduction
Milk has been suggested to be a possible source of oestrogenically active compounds. In order to assess the health risk for milk consumers and ensure the safety of this staple part of the human diet, it is important to study the effect of xenooestrogen mixtures present in milk. This investigation used the available in vivo model to learn to what extent such compounds may be endocrine disruptors.

Material and Methods
The recommended immature golden hamster uterotrophic bioassay was chosen. A total of 132 animals were divided into nine groups of experimental animals and positive and negative control groups, each of 12 animals. The experimental females received ad libitum either one of five samples of raw cow’s milk from individual animals or one of four samples of pasteurised or ultra-high temperature treated cow’s milk as retail products. After 7 days, the animals were sacrificed and necropsied. Uterine weight increases were measured as the endpoint of oestrogenic activity in milk.

Results
The milk samples from individual cows and the retail milk samples did not show oestrogenic activity. However, in three groups, decreased uterine weights were observed.

Conclusion
Considering that milk supplies are beneficial to health, contamination in this food should be avoided. There is a need for further animal experiments and epidemiological studies are warranted to evaluate any causative role of milk in human endocrinological disorders.

<italic>In vivo</italic> study of the oestrogenic activity of milk

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

 study of the oestrogenic activity of milk

{"article-title":{"italic":"In vivo","content":"study of the oestrogenic activity of milk"}}

Introduction
Milk has been suggested to be a possible source of oestrogenically active compounds. In order to assess the health risk for milk consumers and...

In vivo study of the oestrogenic activity of milk

Publicado en línea: 23 sept 2021

Páginas: 335 - 340

Recibido: 30 mar 2021

Aceptado: 26 ago 2021

DOI: https://doi.org/10.2478/jvetres-2021-0049

Palabras clave
raw milk, commercial milk, oestrogenic activity, immature females, hamster uterotrophic assay

© 2021 L. Radko, A. Posyniak. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

In vivo study of the oestrogenic activity of milk

Publicado en línea: 23 sept 2021

Páginas: 335 - 340

Recibido: 30 mar 2021

Aceptado: 26 ago 2021

DOI: https://doi.org/10.2478/jvetres-2021-0049

Palabras claveraw milk, commercial milk, oestrogenic activity, immature females, hamster uterotrophic assay

© 2021 L. Radko, A. Posyniak. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
raw milk, commercial milk, oestrogenic activity, immature females, hamster uterotrophic assay