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The presence of Ehrlichia canis in Rhipicephalus bursa ticks collected from ungulates in continental Eastern Europe

Ioana Adriana Matei

Ioana Adriana Matei

Department of Microbiology, Immunology and EpidemiologyCluj-Napoca, Romania
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Matei, Ioana Adriana
, 
Angela Monica Ionică

Angela Monica Ionică

  • Department of Parasitology and Parasitic Diseases, Faculty of Veterinary MedicineCluj-Napoca, Romania
  • “Regele Mihai I al României” Life Science Institute, University of Agricultural Sciences and Veterinary MedicineCluj-Napoca, Romania
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Ionică, Angela Monica
, 
Alexandra Corduneanu

Alexandra Corduneanu

Department of Parasitology and Parasitic Diseases, Faculty of Veterinary MedicineCluj-Napoca, Romania
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Corduneanu, Alexandra
, 
Cristian Domșa

Cristian Domșa

Department of Parasitology and Parasitic Diseases, Faculty of Veterinary MedicineCluj-Napoca, Romania
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Domșa, Cristian
 y 
Attila D. Sándor

Attila D. Sándor

  • Department of Parasitology and Parasitic Diseases, Faculty of Veterinary MedicineCluj-Napoca, Romania
  • Department of Parasitology and Zoology, University of Veterinary MedicineBudapest, Hungary
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Sándor, Attila D.
  
23 sept 2021
The presence of Ehrlichia canis in Rhipicephalus bursa ticks collected from ungulates in continental Eastern Europe's Cover Image
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Publicado en línea: 23 sept 2021
Páginas: 271 - 275
Recibido: 17 feb 2021
Aceptado: 02 ago 2021
DOI: https://doi.org/10.2478/jvetres-2021-0044
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© 2021 I.A. Matei et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Geographical origin of Rhipicephalus bursa (total collected and Ehrlichia-positive) in south-eastern Romania
Geographical origin of Rhipicephalus bursa (total collected and Ehrlichia-positive) in south-eastern Romania

Fig. 2

Electrophoresis gel image showing positive results and including negative and positive controls and the 100-bp DNA ladder (Invitrogen, part of Thermo-Fisher Scientific, Waltham, MA, USA) as the last 3 lanes
Electrophoresis gel image showing positive results and including negative and positive controls and the 100-bp DNA ladder (Invitrogen, part of Thermo-Fisher Scientific, Waltham, MA, USA) as the last 3 lanes

Fig. 3

Phylogenetic tree of E. canis detected in R. bursa collected from sheep and goats in south-eastern Romania
Phylogenetic tree of E. canis detected in R. bursa collected from sheep and goats in south-eastern Romania

Primers used for the detection of Rickettsiales DNA in Rhipicephalus bursa

Pathogen Primer sequence Target gene Target fragment length Reference
EHR16SD: GGTACCYACAGAAGAAGTCC
Anaplasmataceae EHR16SR: TAGCACTCATCGTTTACAGC 16S rRNA 345bp 19
Ge3a: CACATGCAAGTCGAACGGATTATTC 932bp
Anaplasma spp./ Ge10: TTCCGTTAAGAAGGATCTAATCTCC 16S rRNA 14
A. phagocytophilum Ge2: AACGGATTATTCTTTATAGCTTGCT 546bp
Ge9: GGCAGTATTAAAAGCAGCTCCAGG
ECC: AGAACGAACGCTGGCGGCAAGCC 500bp
Ehrlichia spp./ ECB: CGTATTACCGCGGCTGCTGGCA
E. canis Canis: CAATTATTTATAGCCTCTGGCTATAGGA 16S rRNA 389bp 27
HE3: TATAGGTACCGTCATTATCTTCCCTAT
A. marginale/ Msp43: CCGGATCCTTAGCTGAACAGGAATCTTGC
msp4 842bp 7
A. centrale/A. ovis Msp45: GGGAGCTCCTATGAATTACAGAGAATTGTTTAC
EPLAT5: TTTGTCGTAGCTTGCTATGAT
A. platys EPLAT3: CTTCTGTGGGTACCGTC 16S rRNA 359bp 18
Rsfg877: GGGGGCCTGCT- CACGGCGG
SFG Rickettsia spp. Rsfg1258: ATTGCAAAAAGTACAGTGAACA gltA 359bp 22
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Temas de la revista:
Ciencias de la vida, Biología molecular, Microbiología y virología, Ciencias de la vida, otros, Medicina, Medicina veterinaria
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