This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Fig. 1
PEAKrapid CRL-2828 cells transfected with plasmids expressing bovine IL-4 and bovine GM-CSF together with plasmids expressing GFP
Fig. 2
Expression of rIL-4 and rGM-CSF in culture supernatants of transfected cells. Western blot analysis of total secreted proteins from transfected cells. Total proteins were precipitated with 10 % TCA from culture supernatant of non-transfected cells (1) of cells transfected with plasmid expressing IL-4 (2 and 4) or GM-CSF (3and 5). Molecular weight marker was loaded on lane 6
Fig. 3
Functional activity of culture supernatant of IL-4 and GM-CSF transfected cells. Bovine monocytes were incubated with 10% v/v cell culture supernatant containing rIL-4 and/or rGM-CSF. As a control, monocytes were incubated with 10% v/v cell culture supernatant of non-transfected cells. Cell morphologies were registered with a confocal microscope