Porcine enteric coronavirus infections in wild boar in Poland – a pilot study
Publicado en línea: 22 jul 2021
Páginas: 265 - 269
Recibido: 16 feb 2021
Aceptado: 01 jul 2021
DOI: https://doi.org/10.2478/jvetres-2021-0041
Palabras clave
© 2021 M. Antas et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Introduction
Porcine epidemic diarrhoea virus (PEDV) of the
Material and Methods
In this study, blood and faecal samples from 157 wild boars were collected from 14 provinces of Poland during the 2017–2018 hunting season. RNA was extracted from the faecal homogenate supernatant and subjected to quantitative RT-PCR (RT-qPCR), while clotted blood samples were used for detection of antibodies against PEDV by ELISA.
Results
Five blood samples (3.2%) were seropositive in ELISA, while none of the faecal samples were found positive using RT-qPCR assays.
Conclusion
The results of this analysis indicate the need for additional studies incorporating a larger number of samples and preferably comparing different serological methods, to confirm whether wild boars in Poland act as PEDV reservoirs.