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Comparative expression analysis of inflammatory and immune-related genes in cattle during acute infection with foot-and-mouth disease virus in Egypt

Abeer F. El Nahas
Abeer F. El Nahas
, 
Walaa S.H. Abd El Naby
Walaa S.H. Abd El Naby
, 
Shymaa A. Khatab
Shymaa A. Khatab
, 
Al-Zahraa A. Fergany
Al-Zahraa A. Fergany
 y 
Rashed R. Rashed
Rashed R. Rashed
   | 26 ene 2021
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Publicado en línea: 26 ene 2021
Páginas: 39 - 44
Recibido: 14 jul 2020
Aceptado: 29 dic 2020
DOI: https://doi.org/10.2478/jvetres-2021-0005
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© 2021 A.F. El Nahas et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Electrophoresis of PCR product of FMD virus. A: FMDV universal primer; B: O serotype; C: SAT2 serotype in clinically infected cattle; D: PCR product of bovine HPRT1 from vesicular fluid; M – DNA Ladder (1.5 Kb); lanes 1–3 – PCR products
Electrophoresis of PCR product of FMD virus. A: FMDV universal primer; B: O serotype; C: SAT2 serotype in clinically infected cattle; D: PCR product of bovine HPRT1 from vesicular fluid; M – DNA Ladder (1.5 Kb); lanes 1–3 – PCR products

Fig. 2

The relative expression level of some inflammatory and immune-related genes in cattle infected with FMD virus (O serotype) in two different serotypes: O serotype 1 in February 2017 and O serotype 2 in November 2107. The fold change of the expressed genes was calculated relative to the control group (non-vaccinated and uninfected). The fold change for the control group was 1
The relative expression level of some inflammatory and immune-related genes in cattle infected with FMD virus (O serotype) in two different serotypes: O serotype 1 in February 2017 and O serotype 2 in November 2107. The fold change of the expressed genes was calculated relative to the control group (non-vaccinated and uninfected). The fold change for the control group was 1

Fig. 3

Relative expression levels of some inflammatory and immune-related genes in cattle infected with FMD virus (SAT-2 -serotype) serotypes 1 and 2, representing two different farms. The fold change of the expressed genes was calculated relative to the control group (non-vaccinated and uninfected). The fold change for the control group was 1
Relative expression levels of some inflammatory and immune-related genes in cattle infected with FMD virus (SAT-2 -serotype) serotypes 1 and 2, representing two different farms. The fold change of the expressed genes was calculated relative to the control group (non-vaccinated and uninfected). The fold change for the control group was 1

Serotype-specific primer sequences for FMD virus detection

Primer Sequence 5′–3′ Amplicon size (bp) Annealing temp (°C)
Universal FMD F - CCTACCTCCTTCAACTACGG 216 48
primer R - GAAGGGCCCAGGGTTGGACTC
Serotype O F - AGCTTGTACCAGGGTTTGGC 633 46
R - GCTGCCTACCTCCTTCAA
Serotype SAT2 F - TGGGACACMGGIYTGAACTC 1145 50
R - ACAGCGGCCATGCACGACAG
Serotype A F - TACCAAATTACACACGGGAA 863–866 55
R - GACATGTCCTCCTGCATCTG

Cattle primer sequences for gene expression

Primer Sequence 5′–3′ Amplicon size (bp) Annealing temp (°C)
CXCL10 F - AGTGGAAGCCCCTGCAGTAAA 117 56
R - AGTCCCAGCCTTGCTACTGACA
IL-10 F - CCAAGCCTTGTCGGAAATGA 94 60
R - GTTCACGTGCTCCTTGATGTCA
IFNG F - TCTTCTCAAGCCTCAAGTAACAAGT 103 56
R - CCATGAGGGCATTGGCATAC
HPRT F - GCCGACCTGTTGGATTACAT 289 56
R - ACACTTCGAGGGGTCCTTTT
TLR-2 F - CCATTGACAAGAAGGCCAT 107 56
R - AACCCTTCCTGCTGAGTCTCAT
NFATC4 F - AACCACTGCCCCTCTCTGAAAC 107 56
R - CCTCGACCCCAGATCACAAAGA
eISSN:
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Calendario de la edición:
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Temas de la revista:
Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
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