<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jvetres-2020-0068_s_005_w2aab3b7c29b1b6b1aab1c14b1Aa"><h3>Introduction</h3><p>Early detection of pregnancy is vital for appropriate reproductive management programmes to facilitate the rapid re-insemination of non-pregnant females and reduce the calving interval.</p></sec><sec id="j_jvetres-2020-0068_s_006_w2aab3b7c29b1b6b1aab1c14b2Aa"><h3>Material and Methods</h3><p>A barium chloride test was compared with a commercial progesterone ELISA to detect pregnancy in non-descriptive cows and investigate if it could be applied as an alternative to ELISA in the field. Blood and urine samples were collected from 74 cows with recorded insemination dates. The progesterone ELISA and barium chloride assay were implemented to detect progesterone (P4) in blood and urine specimens, respectively. The cows' reproductive systems were examined after they were slaughtered to determine the uterus's status. Macroscopic examination of the uterus was used as a reference standard for both tests.</p></sec><sec id="j_jvetres-2020-0068_s_007_w2aab3b7c29b1b6b1aab1c14b3Aa"><h3>Results</h3><p>The sensitivity rates of the P4 ELISA and barium chloride test to detect pregnant cows were 100.0% and 79.4%, and to detect the corpus luteum (CL) were 83.0% and 87.0%, respectively, their sensitivity increasing in the presence of the CL. The ELISA and barium chloride tests were 79.7% and 52.7% accurate in the diagnosis of pregnancy. The accuracy of the barium chloride test in CL detection increased to 81.0%, and that of the ELISA to 86.4%. There were no significant differences (P = 0.052) between the barium chloride assay and ELISA when they were utilised for the identification of the CL.</p></sec><sec id="j_jvetres-2020-0068_s_008_w2aab3b7c29b1b6b1aab1c14b4Aa"><h3>Conclusion</h3><p>The barium chloride test can be an inexpensive and time-saving alternative to ELISA in pregnancy diagnosis when the insemination date is known.</p></sec></abstract>

IntroductionEarly detection of pregnancy is vital for appropriate reproductive management programmes to facilitate the rapid re-insemination of non-pregnant females and reduce the calving interval.Material and MethodsA barium chloride test was compared with a commercial progesterone ELISA to detect pregnancy in non-descriptive cows and investigate if it could be applied as an alternative to ELISA in the field. Blood and urine samples were collected from 74 cows with recorded insemination dates. The progesterone ELISA and barium chloride assay were implemented to detect progesterone (P4) in blood and urine specimens, respectively. The cows' reproductive systems were examined after they were slaughtered to determine the uterus's status. Macroscopic examination of the uterus was used as a reference standard for both tests.ResultsThe sensitivity rates of the P4 ELISA and barium chloride test to detect pregnant cows were 100.0% and 79.4%, and to detect the corpus luteum (CL) were 83.0% and 87.0%, respectively, their sensitivity increasing in the presence of the CL. The ELISA and barium chloride tests were 79.7% and 52.7% accurate in the diagnosis of pregnancy. The accuracy of the barium chloride test in CL detection increased to 81.0%, and that of the ELISA to 86.4%. There were no significant differences (P = 0.052) between the barium chloride assay and ELISA when they were utilised for the identification of the CL.ConclusionThe barium chloride test can be an inexpensive and time-saving alternative to ELISA in pregnancy diagnosis when the insemination date is known.

Comparison of a barium chloride test with ELISA for pregnancy detection in cows

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Comparison of a barium chloride test with ELISA for pregnancy detection in cows"}

IntroductionEarly detection of pregnancy is vital for appropriate reproductive management programmes to facilitate the rapid re-insemination of non-pregnant...

	Progesterone ELISA (number (%))				Barium chloride test (number (%))				Total

	Correct positive	Correct negative	False- positive	False- negative	Correct positive	Correct negative	False- positive	False-negative
Pregnancy	34 (45.9)	25 (33.8)	15 (20.3)	0	27 (36.5)	12 (16.2)	28 (37.8)	7 (9.5)	74 (100.0)
CL	49 (66.2)	15 (20.3)	0	10 (13.5)	47 (63.5)	13 (17.6)	7 (9.5)	7 (9.5)	74 (100.0)

Method of detection		Sensitivity	Specificity	PPV	NPV	Accuracy
Progesterone ELISA	Pregnancy	100.0	62.5	69.4	100.0	79.7
	CL	83.0	100.0	100.0	60.0	86.4
Barium chloride test	Pregnancy	79.4	30.0	49.0	63.0	52.7
	CL	87.0	65.0	87.0	65.0	81.0

Comparison of a barium chloride test with ELISA for pregnancy detection in cows

Publicado en línea: 20 oct 2020

Páginas: 567 - 571

Recibido: 26 mar 2020

Aceptado: 05 oct 2020

DOI: https://doi.org/10.2478/jvetres-2020-0068

Palabras clave
barium chloride, cows, corpus luteum, pregnancy diagnosis, progesterone

© 2020 O.I. Dana et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Correct positive, correct negative, false positive, and false-negative results by progesterone ELISA and barium chloride tests for detection of pregnancy and corpus luteum in cows

Values of sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of progesterone ELISA-and barium chloride tests for detection of pregnancy and corpus luteum

Comparison of a barium chloride test with ELISA for pregnancy detection in cows

Publicado en línea: 20 oct 2020

Páginas: 567 - 571

Recibido: 26 mar 2020

Aceptado: 05 oct 2020

DOI: https://doi.org/10.2478/jvetres-2020-0068

Palabras clavebarium chloride, cows, corpus luteum, pregnancy diagnosis, progesterone

© 2020 O.I. Dana et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Correct positive, correct negative, false positive, and false-negative results by progesterone ELISA and barium chloride tests for detection of pregnancy and corpus luteum in cows

Values of sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of progesterone ELISA-and barium chloride tests for detection of pregnancy and corpus luteum

Palabras clave
barium chloride, cows, corpus luteum, pregnancy diagnosis, progesterone