<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2020-0046_s_005">
<h3>Introduction</h3>
<p>Enteropathogenic <italic>Escherichia coli</italic> (EPEC) is one of the main pathotypes causing gastroenteritis, particularly in young immunocompromised hosts. The study reports the prevalence, characterisation, and molecular epidemiology of EPEC from piglets in northeastern India.</p></sec>
<sec id="j_jvetres-2020-0046_s_006">
<h3>Material and Methods</h3>
<p>A total of 457 faecal samples were collected, from which 1,286 <italic>E. coli</italic> strains were isolated and screened by PCR. The resultant EPEC strains were serotyped and phenotypically characterised for resistance against 15 antimicrobials. Also, the phylogenetic sequence was analysed for 11 selected strains.</p></sec>
<sec id="j_jvetres-2020-0046_s_007">
<h3>Results</h3>
<p>A total of 42 strains (3.26%) belonged to atypical EPEC, of which, 15 (35.71%, and 2.29% of the 654 strains from this farm type) were isolated from organised and 27 (64.29%, and 4.27% of the 632 strains from this farm type) from unorganised farms; further, 5 (11.90% of the EPEC strains and 1.51% of the 330 strains from this breed) were isolated from the indigenous breeds and 37 (88.10%, and 3.87% of the 956 strains from this breed) from crossbred piglets. Serogroups O111 (11.9%) and O118 (7.14%) were the most prevalent of the 10 present. Sequence analysis of a length of the <italic>eaeA</italic> gene of 11 isolates of the region showed them to have 100% homology with each other and their identity ranged from 99.4% to 99.7% with GenBank reference sequences. All the EPEC isolates were multi-drug resistant, showing the highest resistance to amoxicillin (80.9%) and cephalexin (76.19%).</p></sec>
<sec id="j_jvetres-2020-0046_s_008">
<h3>Conclusion</h3>
<p>The study highlighted the association of EPEC with piglet’s diarrhoea in northeastern India. EPEC isolates belonged to many serotypes and phenotypically all were multi-drug resistant with close genetic homology.</p></sec>
</abstract>

Introduction
Enteropathogenic Escherichia coli (EPEC) is one of the main pathotypes causing gastroenteritis, particularly in young immunocompromised hosts. The study reports the prevalence, characterisation, and molecular epidemiology of EPEC from piglets in northeastern India.

Material and Methods
A total of 457 faecal samples were collected, from which 1,286 E. coli strains were isolated and screened by PCR. The resultant EPEC strains were serotyped and phenotypically characterised for resistance against 15 antimicrobials. Also, the phylogenetic sequence was analysed for 11 selected strains.

Results
A total of 42 strains (3.26%) belonged to atypical EPEC, of which, 15 (35.71%, and 2.29% of the 654 strains from this farm type) were isolated from organised and 27 (64.29%, and 4.27% of the 632 strains from this farm type) from unorganised farms; further, 5 (11.90% of the EPEC strains and 1.51% of the 330 strains from this breed) were isolated from the indigenous breeds and 37 (88.10%, and 3.87% of the 956 strains from this breed) from crossbred piglets. Serogroups O111 (11.9%) and O118 (7.14%) were the most prevalent of the 10 present. Sequence analysis of a length of the eaeA gene of 11 isolates of the region showed them to have 100% homology with each other and their identity ranged from 99.4% to 99.7% with GenBank reference sequences. All the EPEC isolates were multi-drug resistant, showing the highest resistance to amoxicillin (80.9%) and cephalexin (76.19%).

Conclusion
The study highlighted the association of EPEC with piglet’s diarrhoea in northeastern India. EPEC isolates belonged to many serotypes and phenotypically all were multi-drug resistant with close genetic homology.

Characterisation of porcine enteropathogenic <italic>Escherichia coli</italic> isolated in northeastern India

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Characterisation of porcine enteropathogenic  isolated in northeastern India

Introduction
Enteropathogenic Escherichia coli (EPEC) is one of the main pathotypes causing gastroenteritis, particularly in young immunocompromised hosts. The...

Antibiotic disc and content	Sensitive (%)	Resistant (%)
Ampicillin (AMP) 10 μg	19 (45.23)	23 (54.76)
Amoxicillin (AMX) 30 μg	8 (19.04)	34 (80.90)
Aztreonam (AT) 30 μg	38 (90.47)	4 (9.52)
Cephalexin (CN) 30 μg	10 (23.80)	32 (76.19)
Ceftazidime (CAZ) 30 μg	37 (88.09)	5 (11.90)
Cefixime (CFM) 5 μg	35 (83.33)	7 (16.66)
Ceftriaxone (CTR) 30 μg	40 (95.23)	2 (4.76)
Cefotaxime (CTX) 30 μg	38 (90.47)	4 (9.52)
Ciprofloxacin (CIP) 5 μg	40 (95.23)	2 (4.76)
Enrofloxacin (EX) 10 μg	18 (42.85)	24 (57.14)
Gentamicin (GEN) 10 μg	37 (88.09)	5 (11.90)
Imipenem (IPM) 10 μg	42 (100.00)	0 (0.00)
Nalidixic acid (NA) 30 μg	32 (76.19)	10 (23.80)
Piperacillin (PI) 10 μg	26 (61.90)	16 (38.09)
Streptomycin (S) 10 μg	39 (92.85)	3 (7.14)

Characterisation of porcine enteropathogenic Escherichia coli isolated in northeastern India

Publicado en línea: 14 jul 2020

Páginas: 391 - 397

Recibido: 04 oct 2019

Aceptado: 26 jun 2020

DOI: https://doi.org/10.2478/jvetres-2020-0046

Palabras clave
piglets, EPEC, virulence, serotypes, drug resistance

© 2020 H. Kylla et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

NCBI accession numbers for nucleotide sequences of the eaeA gene of different EPEC strains

Antimicrobial drugs resistance pattern of EPEC from diarrhoeic faecal samples from piglets

No.	Organism	Serotype	GenBank accession number
01	E. coli	O1	KP987181
02	E. coli	O4	KP987182
03	E. coli	Rough	KP987183
04	E. coli	O1	KP987184
05	E. coli	Untypable	KP987185
06	E. coli	Untypable	KP987186
07	E. coli	O1	KP987187
08	E. coli	Untypable	KP987188
09	E. coli	Rough	KP987189
10	E. coli	O111	KP987190
11	E. coli	O111	KP987191

Characterisation of porcine enteropathogenic Escherichia coli isolated in northeastern India

Publicado en línea: 14 jul 2020

Páginas: 391 - 397

Recibido: 04 oct 2019

Aceptado: 26 jun 2020

DOI: https://doi.org/10.2478/jvetres-2020-0046

Palabras clavepiglets, EPEC, virulence, serotypes, drug resistance

© 2020 H. Kylla et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

Fig. 2

NCBI accession numbers for nucleotide sequences of the eaeA gene of different EPEC strains

Antimicrobial drugs resistance pattern of EPEC from diarrhoeic faecal samples from piglets

Palabras clave
piglets, EPEC, virulence, serotypes, drug resistance