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Molecular study of Cysticercus tenuicollis from slaughtered sheep in Sulaymaniyah province, Iraq

Aram Ahmad Mohammed
Aram Ahmad Mohammed
   | 12 may 2020
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Publicado en línea: 12 may 2020
Páginas: 275 - 280
Recibido: 07 sept 2019
Aceptado: 04 may 2020
DOI: https://doi.org/10.2478/jvetres-2020-0034
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© 2020 Aram Ahmad Mohammed. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Fig. 1

PCR amplification of 12S rRNA gene. The12S rRNA gene (489 bp) of C. tenuicollis was amplified and resolved on 1.5% agarose gel. Lane M –marker (100-bp ladder DNA); lane S – positive C. tenuicollis isolate
PCR amplification of 12S rRNA gene. The12S rRNA gene (489 bp) of C. tenuicollis was amplified and resolved on 1.5% agarose gel. Lane M –marker (100-bp ladder DNA); lane S – positive C. tenuicollis isolate

Fig. 2

DNA purification of 12S rRNA gene. PCR products of 12S rRNA gene were recovered and electrophoresed on 1.5% agarose gel. Lane M – marker (100-bp ladder DNA); lanes S1-S15 – positive C. tenuicollis isolates
DNA purification of 12S rRNA gene. PCR products of 12S rRNA gene were recovered and electrophoresed on 1.5% agarose gel. Lane M – marker (100-bp ladder DNA); lanes S1-S15 – positive C. tenuicollis isolates

Fig. 3

Intraspecific phylogenetic relationship of sheep C. tenuicollis isolates in Iraq. The phylogeny of sheep C. tenuicollis was computed by neighbour joining (NJ) from the partial 12S rRNA gene nucleotide sequences. The scale bar represents 0.05% divergence. Bootstrap values are shown above or below branches. MK858233–MK858251 are GenBank accession numbers representing T. hydatigena sequences identified in this study; N1–N19 isolated C. tenuicollis; H1–H5 haplotypes of C. tenuicollis
Intraspecific phylogenetic relationship of sheep C. tenuicollis isolates in Iraq. The phylogeny of sheep C. tenuicollis was computed by neighbour joining (NJ) from the partial 12S rRNA gene nucleotide sequences. The scale bar represents 0.05% divergence. Bootstrap values are shown above or below branches. MK858233–MK858251 are GenBank accession numbers representing T. hydatigena sequences identified in this study; N1–N19 isolated C. tenuicollis; H1–H5 haplotypes of C. tenuicollis

Fig. 4

Interspecific phylogenetic relationship of C. tenuicollis. Phylogeny of T. hydatigena isolates represents partial 12S rRNA sequences. The scale bar represents the estimated numbers of nucleotide substitutions per nucleotide site
Interspecific phylogenetic relationship of C. tenuicollis. Phylogeny of T. hydatigena isolates represents partial 12S rRNA sequences. The scale bar represents the estimated numbers of nucleotide substitutions per nucleotide site

T. hydatigena and other Taeniid nucleotide sequences used for phylogeny

SpeciesOriginHostAccession numberReference
T. hydatigenaIranSheepKU745527Ibrahimi et al., 2016 unpublished
T. hydatigenaIranSheepKX094339Ibrahimi et al., 2016 unpublished
T. hydatigenaEgyptSheepKU671393Abbas et al., 2016 unpublished
T. hydatigenaChinaSheepGQ228819Jia et al., 2010 (9)
T. hydatigenaChinaDogFJ518620Liu et al., 2011 (14)
T. multicepsIranSheepJQ710646Rostami et al., 2013 (19)
T. ovisIranSheepJX134130Rostami et al., 2012 unpublished
T. saginataIranCattleKC344681Rostami et al., 2012 unpublished
T. soliumChinaPigAF337904Li and Dianwu, 2001 unpublished
E. granulosusPortugalSheepHG975356Beato et al., 2014 unpublished

Pairwise nucleotide variations among the five haplotypes of the 12S rRNA gene (determined using the maximum composite likelihood method)

H5H4H3H2H1
H5e
H4d0.007
H3c0.0070.005
H2b0.0070.0050.005
H1a0.0050.0020.0020.002
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Life Sciences, Molecular Biology, Microbiology and Virology, other, Medicine, Veterinary Medicine
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