<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec id="j_jvetres-2020-0020_s_005">
<h3>Introduction</h3>
<p>Classical swine fever virus (CSFV) causes an economically important and highly contagious disease of pigs, leading to economic losses around the world. Attenuated live vaccines with CSFV antigens have played an important role in the prevention and control of the disease. Porcine kidney 15 (PK15) cells have been widely used for the propagation of CSFV, but this cell line is not efficient or homogeneously susceptible to viral infection.</p></sec>
<sec id="j_jvetres-2020-0020_s_006">
<h3>Material and Methods</h3>
<p>To achieve a homogeneous PK15 cell line which enabled high titre replication of CSFV, we used the limiting dilution cell cloning method.</p></sec>
<sec id="j_jvetres-2020-0020_s_007">
<h3>Results</h3>
<p>We developed two cell clones, PK15-1A6 and PK15-3B1, which respectively have high- and low-permissive phenotypes to CSFV infection. The PK15-1A6, PK15-3B1, and PK15 parent cells showed different characteristics in cell proliferation rate, susceptibility to CSFV infection, and CSFV production. The mean virus titres per millilitre reflected by TCID<sub>50</sub> values in PK15-1A6, PK15-3B1, and PK15 parent cells were 106.85, 103.63, and 104.74, respectively.</p></sec>
<sec id="j_jvetres-2020-0020_s_008">
<h3>Conclusion</h3>
<p>The PK15-1A6 cell clone is more permissive to CSFV infection than the PK15 parent cells. The screened high-permissive cells will be useful for CSFV propagation and vaccine development <italic>in vitro</italic>, and facilitate research on the pathogenicity of CSFV.</p></sec></abstract>

Introduction
Classical swine fever virus (CSFV) causes an economically important and highly contagious disease of pigs, leading to economic losses around the world. Attenuated live vaccines with CSFV antigens have played an important role in the prevention and control of the disease. Porcine kidney 15 (PK15) cells have been widely used for the propagation of CSFV, but this cell line is not efficient or homogeneously susceptible to viral infection.

Material and Methods
To achieve a homogeneous PK15 cell line which enabled high titre replication of CSFV, we used the limiting dilution cell cloning method.

Results
We developed two cell clones, PK15-1A6 and PK15-3B1, which respectively have high- and low-permissive phenotypes to CSFV infection. The PK15-1A6, PK15-3B1, and PK15 parent cells showed different characteristics in cell proliferation rate, susceptibility to CSFV infection, and CSFV production. The mean virus titres per millilitre reflected by TCID50 values in PK15-1A6, PK15-3B1, and PK15 parent cells were 106.85, 103.63, and 104.74, respectively.

Conclusion
The PK15-1A6 cell clone is more permissive to CSFV infection than the PK15 parent cells. The screened high-permissive cells will be useful for CSFV propagation and vaccine development in vitro, and facilitate research on the pathogenicity of CSFV.

Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction
Classical swine fever virus (CSFV) causes an economically important and highly contagious disease of pigs, leading to economic losses around the...

Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus

Publicado en línea: 24 mar 2020

Páginas: 9 - 14

Recibido: 17 jul 2019

Aceptado: 27 feb 2020

DOI: https://doi.org/10.2478/jvetres-2020-0020

Palabras clave
classical swine fever virus, PK15, high-permissive cells, cell cloning, vaccine production

© 2020 M. Yin et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Cloning and identification of PK15 cells for enhanced replication of classical swine fever virus

Publicado en línea: 24 mar 2020

Páginas: 9 - 14

Recibido: 17 jul 2019

Aceptado: 27 feb 2020

DOI: https://doi.org/10.2478/jvetres-2020-0020

Palabras claveclassical swine fever virus, PK15, high-permissive cells, cell cloning, vaccine production

© 2020 M. Yin et al. published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
classical swine fever virus, PK15, high-permissive cells, cell cloning, vaccine production