Acceso abierto

Evaluation of the possibility of C. burnetii transmission by the alimentary route in a guinea pig model

Agnieszka Jodełko

Agnieszka Jodełko

Department of Cattle and Sheep DiseasesPuławy, Poland
Buscar este autor en
Sciendo|Google Scholar
Jodełko, Agnieszka
, 
Monika Szymańska-Czerwińska

Monika Szymańska-Czerwińska

Department of Cattle and Sheep DiseasesPuławy, Poland
Buscar este autor en
Sciendo|Google Scholar
Szymańska-Czerwińska, Monika
, 
Anna Kycko

Anna Kycko

Department of Pathology, National Veterinary Research InstitutePuławy, Poland
Buscar este autor en
Sciendo|Google Scholar
Kycko, Anna
 y 
Krzysztof Niemczuk

Krzysztof Niemczuk

Department of Cattle and Sheep DiseasesPuławy, Poland
Buscar este autor en
Sciendo|Google Scholar
Niemczuk, Krzysztof
  
13 sept 2019
Evaluation of the possibility of C. burnetii transmission by the alimentary route in a guinea pig model's Cover Image
Acerca de este artículo
Artículo anterior
Artículo siguiente
Cite
Descargar portada
Publicado en línea: 13 sept 2019
Páginas: 311 - 315
Recibido: 04 jun 2019
Aceptado: 12 ago 2019
DOI: https://doi.org/10.2478/jvetres-2019-0055
Palabras clave
© 2019 A Jodełko et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Q fever (coxiellosis) is an infectious disease of animals and humans, caused by.C. burnetii and widely distributed throughout the world. It is known that people and animals acquire the disease predominantly.via inhalation of infectious aerosols. The possibility of transmission of the pathogen by the alimentary route is still a matter of debate and remains controversial. Therefore the aim of this study was to fill the gaps in knowledge of oral transmission of.C. burnetii by conducting biological tests on the guinea pig model.

Material and Methods

Guinea pigs, divided into five groups comprising a negative control and four experimental groups, received specified concentrations of.C. burnetii per os. To determine the presence of specific antibodies, blood samples were tested using CFT. Also, internal organs collected during necropsy were screened by a real-time PCR targeting I.1111. Additionally, histopathological evaluation of the tissues was performed.

Results

The presence of antibodies and pathogen DNA in caecum was confirmed in one guinea pig from experimental group IV..C. burnetii was also detected in testicular tissue collected from one animal of experimental group II.

Conclusions

The presence of pathogen DNA in the testicular tissue indicates that infection spreads haematogenously. In the majority of experimental animals specific antibodies and genetic material of.C. burnetii were not detected. This fact suggests that development of infection depends on many factors, such as animal immune status.

Idioma:
Inglés
Calendario de la edición:
4 veces al año
Temas de la revista:
Ciencias de la vida, Biología molecular, Microbiología y virología, Ciencias de la vida, otros, Medicina, Medicina veterinaria
RSS Feed de revista