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Clostridium perfringens spores in Polish honey samples

Tomasz Grenda

Tomasz Grenda

Department of Hygiene of Animal Feedingstuffs, National Veterinary Research InstitutePulawy, Poland
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Grenda, Tomasz
, 
Magdalena Grabczak

Magdalena Grabczak

Department of Hygiene of Animal Feedingstuffs, National Veterinary Research InstitutePulawy, Poland
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Grabczak, Magdalena
, 
Magdalena Goldsztejn

Magdalena Goldsztejn

Department of Hygiene of Animal Feedingstuffs, National Veterinary Research InstitutePulawy, Poland
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Goldsztejn, Magdalena
, 
Nina Kozieł

Nina Kozieł

Department of Hygiene of Animal Feedingstuffs, National Veterinary Research InstitutePulawy, Poland
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Kozieł, Nina
, 
Krzysztof Kwiatek

Krzysztof Kwiatek

Department of Hygiene of Animal Feedingstuffs, National Veterinary Research InstitutePulawy, Poland
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Kwiatek, Krzysztof
, 
Krystyna Pohorecka

Krystyna Pohorecka

Department of Honey Bee Diseases, National Veterinary Research InstitutePulawy, Poland
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Pohorecka, Krystyna
, 
Marta Skubida

Marta Skubida

Department of Honey Bee Diseases, National Veterinary Research InstitutePulawy, Poland
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Skubida, Marta
 y 
Andrzej Bober

Andrzej Bober

Department of Honey Bee Diseases, National Veterinary Research InstitutePulawy, Poland
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Bober, Andrzej
  
23 oct 2018
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Publicado en línea: 23 oct 2018
Páginas: 281 - 284
Recibido: 07 may 2018
Aceptado: 28 ago 2018
DOI: https://doi.org/10.2478/jvetres-2018-0040
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© 2018 T. Grenda et al. published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

The aim of this study was examination of honey samples collected from apiaries situated in all Polish provinces for occurrence of Clostridium spp., especially C. perfringens.

Material and Methods

The study was carried out on 240 honey samples (15 samples/province). Estimation of Clostridium titre, its cultures and C. perfringens isolate characterisation were performed according to the standard PN-R-64791:1994. A multiplex PCR method for detection of genes coding cpa (α toxin), cpb (β), cpb2 (β2), etx (ε), iap (ι), and cpe (enterotoxin) toxins was used.

Results

Clostridium spp. was noticed in 56% (136/240) of samples, and its titres ranged between 0.1 g and 0.001 g. Clostridium perfringens occurrence was evidenced in 27.5% (66/240) of samples. All isolates were classified to toxinotype A.

Conclusions

Evidence of a high number of positive samples with occurrence of Clostridium spp. indicates a potential risk to consumers’ health. The infective number of Clostridium spp. is unknown; however, the obtained results have shown that a risk assessment on the entire honey harvesting process should be made in order to ensure microbiological safety. Moreover, a detailed study should be undertaken on the antibiotic resistance of C. perfringens isolates from honey samples.

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Inglés
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4 veces al año
Temas de la revista:
Ciencias de la vida, Biología molecular, Microbiología y virología, Ciencias de la vida, otros, Medicina, Medicina veterinaria
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