Saponin-based Mycoplasma bovis vaccine containing lysozyme dimer adjuvant stimulates acute phase response in calves

Mycoplasma bovis is known as a causative agent of many disorders in cattle. In Europe, there is still a lack of commercial vaccines against M. bovis infection. Acute phase response (APR) is a non-specific host reaction to infection, most seen in changes in production of acute phase proteins. The aim of this study was to analyse APR in calves administered with an experimental M. bovis vaccine.

Material and Methods

Twelve healthy female calves were divided into two equal groups: experimental and control. The experimental vaccine containing the field M. bovis strain and two adjuvants such as saponin and lysozyme dimer was subcutaneously administered to the experimental group. Phosphate buffered saline was taken as the placebo and given to the control group by the same route as the vaccine. Blood samples were collected prior to the study (day 0), then daily up to day 7, and then each seven days until day 84 post vaccination. The concentrations of serum amyloid A (SAA), haptoglobin (Hp), interferon-γ (IFN-γ), and inteleukin-4 (IL-4) were determined using commercial ELISA kits.

Results

Following the vaccination, a significant increase in SAA, Hp, and IFN-γ concentrations was observed when compared to the unvaccinated calves, whereas the IL-4 concentration was not detectable.

Conclusion

The experimental saponin-based M. bovis vaccine containing lysozyme dimer adjuvant visibly stimulated the APR in the calves, and some specific cytokines (Th1-dependent) directly involved in this response.

Idioma:: Inglés

Calendario de la edición:: 4 veces al año
Temas de la revista:: Ciencias de la vida, Biología molecular, Microbiología y virología, Ciencias de la vida, otros, Medicina, Medicina veterinaria

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Saponin-based Mycoplasma bovis vaccine containing lysozyme dimer adjuvant stimulates acute phase response in calves

Katarzyna Dudek

Dariusz Bednarek

Publicado en línea: 23 oct 2018

Páginas: 269 - 273

Recibido: 29 may 2018

Aceptado: 20 sept 2018

DOI: https://doi.org/10.2478/jvetres-2018-0038

Palabras clavecalves, vaccine, saponin, lysozyme, acute phase proteins, cytokines

© 2018 K. Dudek and D. Bednarek published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Material and Methods

Results

Conclusion

Palabras clave
calves, vaccine, saponin, lysozyme, acute phase proteins, cytokines