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Evaluation of Meloidogyne incognita and Rotylenchulus reniformis nematode-resistant cotton cultivars with supplemental Corteva Agriscience nematicides

A. Kate Turner

A. Kate Turner

Alabama, United States
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Kate Turner, A.
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Scott H. Graham

Scott H. Graham

Alabama, United States
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Graham, Scott H.
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Neha Potnis

Neha Potnis

Alabama, United States
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Potnis, Neha
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Steve M. Brown

Steve M. Brown

Alabama, United States
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Brown, Steve M.
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Pat Donald

Pat Donald

Alabama, United States
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Donald, Pat
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Kathy S. Lawrence

Kathy S. Lawrence

Alabama, United States
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Lawrence, Kathy S.
  
17 feb 2023
Evaluation of Meloidogyne incognita and Rotylenchulus reniformis nematode-resistant cotton cultivars with supplemental Corteva Agriscience nematicides's Cover Image
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Categoría del artículo: Research Paper
Publicado en línea: 17 feb 2023
Recibido: 06 ago 2022
DOI: https://doi.org/10.2478/jofnem-2023-0001
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© 2023 Turner et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

M. incognita PHY 340 W3FE susceptible variety on the left and the resistant PHY 360 W3FE on the right at 106 DAP.
M. incognita PHY 340 W3FE susceptible variety on the left and the resistant PHY 360 W3FE on the right at 106 DAP.

Figure 2

Field trial M. incognita eggs per gram of root collected from four root systems LS means for PHY 360 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2020. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0368. Nematicide treatments included no-nematicide control, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), C-LV (0.28 +1.24 L/ha), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha).
Field trial M. incognita eggs per gram of root collected from four root systems LS means for PHY 360 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2020. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0368. Nematicide treatments included no-nematicide control, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), C-LV (0.28 +1.24 L/ha), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha).

Figure 3

Field trial M. incognita eggs per gram of root collected from four root systems LS means for PHY 360 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2021. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0272. Nematicide treatments included no-nematicide control, Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha) Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha, Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.21 L/ ha) + Vydate C-LV (0.88 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.50 L/ha).
Field trial M. incognita eggs per gram of root collected from four root systems LS means for PHY 360 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2021. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0272. Nematicide treatments included no-nematicide control, Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha) Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha, Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.21 L/ ha) + Vydate C-LV (0.88 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.50 L/ha).

Figure 4

Auburn University’s Tennessee Valley Research Extension Center showing the nematode susceptible PHY 340 W3FE on the left and the R. reniformis-resistant PHY 332 W3FE on the right 102 DAP.
Auburn University’s Tennessee Valley Research Extension Center showing the nematode susceptible PHY 340 W3FE on the left and the R. reniformis-resistant PHY 332 W3FE on the right 102 DAP.

Figure 5

Field trial R. reniformis eggs per gram of root collected from four root systems LS means for PHY 332 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2020. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0178. Nematicide treatments included no-nematicide control, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), C-LV (0.28 +1.24 L/ha), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha).
Field trial R. reniformis eggs per gram of root collected from four root systems LS means for PHY 332 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2020. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0178. Nematicide treatments included no-nematicide control, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), C-LV (0.28 +1.24 L/ha), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha).

Figure 6

Field trial R. reniformis eggs per gram of root collected from four root systems. LS means for PHY 332 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2021. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0441. Nematicide treatments included no-nematicide control, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), C-LV (0.28 +1.24 L/ha), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha).
Field trial R. reniformis eggs per gram of root collected from four root systems. LS means for PHY 332 W3FE (R) and PHY 340 W3FE (S) cotton and nematicide combination at 40 DAP in 2021. P-value for Type III fixed effects for the Variety x Nematicide interaction was 0.0441. Nematicide treatments included no-nematicide control, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), C-LV (0.28 +1.24 L/ha), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), BIOST Nematicide 100, Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha), BIOST Nematicide 100 (0.026 mg ai/seed), Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha).

Field trial LS means for PHY 360 W3FE [M_ incognita resistant (R)] and PHY 340 W3FE [M_ incognita susceptible (S)] cotton variety and nematicide combination effects on plant stand, plant height, root fresh weight, plant biomass, and lint cotton yield at the Plant Breeding Unit, Shorter, AL in 2021_

Source of variation (F-value) Plant stand (13 m row) Plant height (cm) Root fresh weight (g) Biomass (g) Lint yield (cotton kg/ha)
Cultivara 0.0334*b 0.6081 0.7150 0.0430** 0.230****
Nematicidec 0.2073 0.0001**** 0.0001**** 0.0001*** 0.2907***
Cultivar x Nematicide 0.9026 0.7749 0.5254 0.7427 0.6664
Cultivar LS-means
PHY 360 W3FE (R) 81.33d a 16.88 a 3.26 a 42.44 a 1411 a
PHY 340 W3FE (S) 83.69 a 16.73 a 3.22 a 39.31 b 887 b
Nematicide LS-means
No nematicide 82.25 a 15.09 c 2.34 d 30.82 d 964 B
Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha) 82.35 a 16.53 bc 2.96 bcd 37.03 cd 1221 Ab
Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) 84.00 a 16.41 bc 3.18 bcd 40.07 bcd 1002 Ab
Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) 81.50 a 17.36 ab 3.41abc 43.25 abc 1171 Ab
BIOST Nematicide 100e (0.026 mg ai/seed) 84.85 a 14.94 c 2.75 cd 33.15 d 1060 Ab
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.21 L/ha) + Vydate CLV (0.88 L/ha) 82.30 a 17.84 ab 3.71 ab 47.79 ab 1143 Ab
BIOST Nematicide 100(0.026 mg ai/seed) + Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) 83.90 a 17.55 ab 3.41 abc 43.78 abc 1316 A
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) 78.90 a 18.70 a 4.09 a 51.11 a 1315 A

Field trial LS means for PHY 360 W3FE [M_ incognita resistant (R)] and PHY 340 W3FE [M_ incognita susceptible (S)] cotton variety and nematicide combination effects on plant stand, plant height, root fresh weight, plant biomass, and lint cotton yield at the Plant Breeding Unit, Shorter, AL in 2020_

Source of variation (F-value) Plant stand (13 m row) Plant height (cm) Root fresh weight (g) Biomass (g) Lint cotton yield (kg/ha)
Cultivara 0.0001****b 0.5451 0.1338 0.0206* 0.0402**
Nematicidec 0.0001**** 0.1340 0.3412 0.1491 0.001***
Cultivar x Nematicide 0.3891 0.9234 0.9204 0.9481 0.3753
Cultivar LS-means
PHY 360 W3FE (R) 44.58d b 13.91 a 0.49 a 4.57 b 1621 a
PHY 340 W3FE (S) 71.58 a 13.69 a 0.52 a 5.06 a 1529 b
Nematicide LS-means
No nematicide 63.25 a 14.13 a 0.50 a 4.74 a 1527 ab
Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) 60.65 a 14.79 a 0.52 a 5.17 a 1489 ab
Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) 45.65 b 13.43 a 0.49 a 4.22 a 1363 b
Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha) 48.00 b 13.20 a 0.54 a 4.77 a 1458 ab
BIOST Nematicide 100e (0.026 mg ai/seed) 61.35 a 12.74 a 0.47 a 4.41 a 1657 a
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) 63.45 a 14.15 a 0.51 a 5.33 a 1716 a
BIOST Nematicide 100(0.026 mg ai/seed) + Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) 62.70 a 13.74 a 0.50 a 4.81 a 1720 a
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha) 59.55 a 14.25 a 0.53 a 5.08 a 1671 a

Field trial LS means for PHY 332 W3FE [R_ reniformis-resistant (R)] and PHY 340 W3FE [R_ reniformis-susceptible (S)] cotton variety and nematicide combination effects on plant stand, plant height, root fresh weight, plant biomass, and lint cotton yield at the Tennessee Valley Research and Extension Center, Belle Mina, AL in 2021_

Source of variation (F-value) Plant stand (13 m row) Plant height (cm) Root fresh weight (g) Biomass (g) Lint cotton yield (kg/ha)
Cultivara 0.0001****b 0.8351 0.1794 0.0207** 0.0001****
Nematicidec 0.0209** 0.0001*** 0.0001**** 0.0001**** 0.0001****
Cultivar x Nematicide 0.1123 0.9458 0.7584 0.7887 0.8616
Cultivar LS-means
PHY 332 W3FE (R) 40.85d a 11.22 a 1.20 a 12.46 A 1692 a
PHY 340 W3FE (S) 24.81 b 12.42 a 1.10 a 11.07 B 989 b
Nematicide LS-means
No nematicide 30.07 ab 9.68 c 0.74 c 8.07 C 1008 c
Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha) 39.62 a 10.61 abc 1.10 abc abc 10.90 1253 bc
Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) 34.26 ab 11.64 ab 1.30 a 12.96 ab 1456 ab
Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) 35.60 ab 11.48 abc 1.32 a 12.49 ab 1445 ab
BIOST Nematicide 100e (0.026 mg ai/seed) 32.43 ab 10.28 bc 0.87 bc 9.30 bc 1099 c
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.21 L/ha) + Vydate C-LV (0.88 L/ha) 32.37 ab 12.15 a 1.33 a 13.55 A 1334 abc
BIOST Nematicide 100(0.026 mg ai/seed) + Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) 30.19 ab 12.00 ab 1.35 a 14.23 A 1538 ab
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) 28.10 b 11.63 ab 1.20 ab 12.62 ab 1591 a

Cotton (Gossypium hirsutum) cultivars grouped into M_ incognita (Mi) or R_ reniformis (Rr) resistance categories by company_

Cultivar Mi resistance Rr resistance Company
Fibermax 1621 GL PRa BASF; Ludwigshafen, Germany
Fibermax 1911 GLT PR BASF; Ludwigshafen, Germany
Fibermax 2011 GT PR BASF; Ludwigshafen, Germany
Fibermax 1730 GLTP PR BASF; Ludwigshafen, Germany
Stoneville 4946 GLB2 PR BASF; Ludwigshafen, Germany
Stoneville 5600 B2XF PR BASF; Ludwigshafen, Germany
Deltapine 2141 NR B3XF PR PR Bayer; Leverkusen, Germany
Deltapine 2143 NR B3XF PR PR Bayer; Leverkusen, Germany
Deltapine 1454NR B2RF NRb Bayer; Leverkusen, Germany
Deltapine 1558NR B2RF NR Bayer; Leverkusen, Germany
Deltapine 1747NR B2RF NR Bayer; Leverkusen, Germany
Deltapine 1823NR B2XF NR Bayer; Leverkusen, Germany
PhytoGen 250 W3FE PR Corteva AgriScience; Indianapolis, IN
PhytoGen 332 W3FE Rc R Corteva AgriScience; Indianapolis, IN
PhytoGen 350 W3FE PR Corteva AgriScience; Indianapolis, IN
PhytoGen 390 W3FE R Corteva AgriScience; Indianapolis, IN
PhytoGen 400 W3FE PR Corteva AgriScience; Indianapolis, IN
PhytoGen 430 W3FE PR Corteva AgriScience; Indianapolis, IN
PhytoGen 440 W3FE PR Corteva AgriScience; Indianapolis, IN
PhytoGen 443 W3FE R R Corteva AgriScience; Indianapolis, IN
PhytoGen 545 W3FE R Corteva AgriScience; Indianapolis, IN
PhytoGen 320 W3FE PR Corteva AgriScience; Indianapolis, IN
PhytoGen 360 W3FE R Corteva AgriScience; Indianapolis, IN
PhytoGen 417 WRF HRd Corteva AgriScience; Indianapolis, IN
PhytoGen 427 W3FE R Corteva AgriScience; Indianapolis, IN
PhytoGen 480 W3FE HR Corteva AgriScience; Indianapolis, IN
PhytoGen 530 W3FE R Corteva AgriScience; Indianapolis, IN
PhytoGen 580 W3FE HR Corteva AgriScience; Indianapolis, IN

Field trial LS means for PHY 332 W3FE [R_ reniformis-resistant (R)] and PHY 340 W3FE [R_ reniformis-susceptible (S)] cotton variety and nematicide combination effects on plant stand, plant height, root fresh weight, plant biomass, and lint cotton yield at the Tennessee Valley Research and Extension Center, Belle Mina, AL in 2020_

Source of variation (F-value) Plant stand (13 m row) Plant height (cm) Root fresh weight (g) Biomass (g) Lint cotton yield (kg/ha)
Cultivara 0.0001****b 0.0139*** 0.0004**** 0.0001**** 0.0001****
Nematicidec 0.0732* 0.0001*** 0.0001**** 0.0001*** 0.0001****
Cultivar x Nematicide 0.0966 0.3482 0.9314 0.9473 0.1298
Cultivar LS-means
PHY 332 W3FE (R) 51.20d a 13.42 a 2.52 a 21.82 A 1998 b
PHY 340 W3FE (S) 41.34 b 12.42 b 1.97 b 16.62 B 1284 a
Nematicide LS-means
No nematicide 51.20 a 11.73 c 1.72 c 14.32 B 1289 c
Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) 46.85 a 13.33 abc 2.14 abc 19.29 ab 1545 ab
Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) 50.35 a 14.10 a 2.64 a 23.70 A 1732 ab
Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha) 46.70 a 12.63 abc 1.98 bc 18.13 ab 1668 ab
BIOST Nematicide 100e (0.026 mg ai/seed) 46.80 a 12.36 bc 2.1 abc 18.08 ab 1504 bc
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (0.28 L/ha) + Vydate C-LV (1.24 L/ha) 40.20 a 12.59 abc 2.12 abc 17.67 ab 1687 ab
BIOST Nematicide 100(0.026 mg ai/seed) + Reklemel (0.56 L/ha) + Vydate C-LV (2.5 L/ha) 45.10 a 13.45 ab 2.71 a 21.95 A 1777 a
BIOST Nematicide 100 (0.026 mg ai/seed) + Reklemel (1.13 L/ha) + Vydate C-LV (5.0 L/ha) 42.95 a 13.16 abc 2.57 ab 20.60 A 1731 ab

Cotton cultivar PHY 340 W3FE [susceptible(S)], PHY 360 W3FE [M_ incognita resistant(R)] and PHY 332 W3FE [R_ reniformis resistant (R)] and nematicide rates and combinations used in field experiments conducted at the Plant Breeding Unit (PBU), Shorter, AL and Tennessee Valley Research and Extension Center (TVREC), Belle Mina, AL in 2020 and 2021_

Number Cotton cultivar Nematicides 2020 rates 2021 rates Application
1 PHY 340 W3FE (S) No nematicide
2 PHY 340 W3FE (S) Reklemela + Vydate C-LVb 0.28 + 1.24 L/ha 0.21 + 0.88 L/ ha In-furrow spray
3 PHY 340 W3FE (S) Reklemel + Vydate C-LV 0.56 + 2.5 L/ha 0.28 + 1.24 L/ ha In-furrow spray
4 PHY 340 W3FE (S) Reklemel + Vydate C-LV 1.13 + 5.0 L/ha 0.56 + 2.50 L/ ha In-furrow spray
5 PHY 340 W3FE (S) BIOST Nematicide 100c 0.026 mg ai/seed 0.026 mg ai/ seed Seed treatment
6 PHY 340 W3FE (S) BIOST Nematicide 100 + Reklemel + Vydate C-LV 0.026 mg ai/seed + 0.28 + 1.24 L/ ha 0.026 mg ai/ seed + 0.21 + 0.884 L/ha Seed treatment + In-furrow spray
7 PHY 340 W3FE (S) BIOST Nematicide 100 + Reklemel + Vydate C-LV 0.026 mg ai/seed + 0.56 + 2.50 L/ ha 0.026 mg ai/ seed + 0.28 + 1.24 L/ha Seed treatment + In-furrow spray
8 PHY 340 W3FE (S) BIOST Nematicide 100 + Reklemel + Vydate C-LV 0.026 mg ai/seed + 1.13 + 5.0 L/ha 0.026 mg ai/ seed + 1.13 + 5.0 L/ha Seed treatment + In-furrow spray
9 PHY 360 W3FE (R) or PHY 332 W3FE (R)c No nematicide
10 PHY 360 W3FE (R) or PHY 332 W3FE (R) Reklemel + Vydate C-LV 0.28 + 1.24 L/ha 0.21 + 0.88 L/ ha In-furrow spray
11 PHY 360 W3FE (R) or PHY 332 W3FE (R) Reklemel + Vydate C-LV 0.56 + 2.5 L/ha 0.28 + 1.24 L/ ha In-furrow spray
12 PHY 360 W3FE (R) or PHY 332 W3FE (R) Reklemel + Vydate C-LV 1.13 + 5.0 L/ha 0.56 + 2.50 L/ ha In-furrow spray
13 PHY 360 W3FE (R) or PHY 332 W3FE (R) BIOST Nematicide 100e 0.026 mg ai/seed 0.026 mg ai/ seed Seed treatment
14 PHY 360 W3FE (R) or PHY 332 W3FE (R) BIOST Nematicide 100 + Reklemel + Vydate C-LV 0.026 mg ai/seed + 0.28 + 1.24 L/ ha 0.026 mg ai/ seed + 0.21 + 0.88 L/ha Seed treatment + In-furrow spray
15 PHY 360 W3FE (R) or PHY 332 W3FE (R) BIOST Nematicide 100 + Reklemel + Vydate C-LV 0.026 mg ai/seed + 0.56 + 2.50 L/ ha 0.026 mg ai/ seed + 0.28 + 1.24 L/ha Seed treatment + In-furrow spray
16 PHY 360 W3FE (R) or PHY 332 W3FE (R) BIOST Nematicide 100 + Reklemel + Vydate C-LV 0.026 mg ai/seed + 1.13 + 5.0 L/ha 0.026 mg ai/ seed + 0.56 + 5.0 L/ha Seed treatment + In-furrow spray

Greenhouse trial LS means for PHY 360 W3FE, M_ incognita resistant (R), PHY 332 W3FE, R_ reniformis resistant (R), each paired with PHY 340 W3FE, susceptible (S) cotton variety effects on plant height, shoot fresh weight, root fresh weight, biomass, total nematode eggs, and nematodes eggs per gram of root at 30 DAP at the Plant Science Research Center, Auburn, AL in 2020 and 2021_

Cotton cultivar Plant height (cm) Shoot fresh weight (g) Root fresh weight (g) Biomass (g) Total M. incognita eggs M. incognita eggs/g of roota
PHY 360 W3FE 12.63 A 6.55 a 4.31 b 10.85 a 194 b 42 B
PHY 340 W3FEb 11.95 ac 6.21 a 6.68 a 12.89 a 1922 a 353 A
P-values 0.4848 0.6348 0.0024 0.0749 0.0001 0.0009
Cotton cultivar Plant height (cm) Shoot fresh weight (g) Root fresh weight (g) Biomass (g) Total R. reniformis eggs R. reniformis eggs/g of root
PHY 332 W3FE 11.02 A 5.5 a 4.57 a 10.06 a 447 b 146 A
PHY 340 W3FE 11.97 A 6.24 a 6.55 a 12.79 a 1935 a 358 A
P-values 0.3803 0.3223 0.0797 0.0871 0.0003 0.0810
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