<abstract xmlns="http://www.w3.org/1999/xhtml">The nematicidal activity of radish bulb (<italic>Raphanus sativus</italic>) methanol (RME) and aqueous extracts (RAE) was tested against the second stage (J2) root knot nematode <italic>Meloidogyne incognita</italic> model. The EC50 of RME after 3 d of J2 immersion in test solutions was 312 ± 65 μg/ml. However, no activity was noted for RAE (EC50 > 1,000 μg/ml). The chemical composition analysis of the methanol extract carried out by the GC–MS technique showed that 4-methylthio-3-butenyl isothiocyanate (raphasatin) was the most abundant compound at 20%. This pure compound strongly induced J2 paralysis with an EC50 of 1.3 ± 0.2 μg/ml after 24 hr. Comparison with other 11 selected isothiocyanates with structure similarity showed that the double bond at position 3 of the alkyl side chain is important for nematicidal activity, followed by the sulfur group at position 5 and the benzyl group at position 2. In addition, raphasatin showed the highest nematicidal activity with the corresponding lowest antioxidant activity of about 92 ± 18 μg/ml. In conclusion, the results of this investigation reveal that <italic>R. sativus</italic> and its major compound raphasatin can be integrated into the pest management system.</abstract>

The nematicidal activity of radish bulb (Raphanus sativus) methanol (RME) and aqueous extracts (RAE) was tested against the second stage (J2) root knot nematode Meloidogyne incognita model. The EC50 of RME after 3 d of J2 immersion in test solutions was 312 ± 65 μg/ml. However, no activity was noted for RAE (EC50 > 1,000 μg/ml). The chemical composition analysis of the methanol extract carried out by the GC–MS technique showed that 4-methylthio-3-butenyl isothiocyanate (raphasatin) was the most abundant compound at 20%. This pure compound strongly induced J2 paralysis with an EC50 of 1.3 ± 0.2 μg/ml after 24 hr. Comparison with other 11 selected isothiocyanates with structure similarity showed that the double bond at position 3 of the alkyl side chain is important for nematicidal activity, followed by the sulfur group at position 5 and the benzyl group at position 2. In addition, raphasatin showed the highest nematicidal activity with the corresponding lowest antioxidant activity of about 92 ± 18 μg/ml. In conclusion, the results of this investigation reveal that R. sativus and its major compound raphasatin can be integrated into the pest management system.

Nematicidal effect of Raphasatin from <italic>Raphanus sativus</italic> against <italic>Meloidogyne incognita</italic>

Journal of Nematology

This work is licensed under the Creative Commons Attribution 4.0 International License.

Nematicidal effect of Raphasatin from  against 

The nematicidal activity of radish bulb (Raphanus sativus) methanol (RME) and aqueous extracts (RAE) was tested against the second stage (J2) root knot...

Compound	MW (g/mol)	EC_{50/24 h} (μg/ml)	EC₅₀ DPPH (μg/ml)
Raphasatin	159.27	1.3 ± 0.2	92 ± 18
Benzylisothiocyanate	149.21	1.9 ± 0.3^a	90 ± 17
Erucin	165.21	3.2 ± 1.7^a	43 ± 12
Allylisothiocyanate	99.15	6.6 ± 2.5^a	32 ± 4.3
Methylisothiocyanate	73.12	7.9 ± 3.1^a	20 ± 2.6
Pentylisothiocyanate	129.23	11.1 ± 5.0^a	9.4 ± 1.7
Hexylisothiocyanate	127.18	11.3 ± 2.6^a	nt
Butylisothiocyanate^c	115.20	12 ± 8.0^a	9.1 ± 1.5
Sulforaphane	177.3	152 ± 35^a	nt
Iberin	163.3	180 ± 21^a	6.55 ± 1.1
Isobutylisothiocyanate	115.20	>200^a	5.3 ± 0.9
Phenylisothiocyanate	135.19	>1,000^a	2.6 ± 0.7
Fosthiazate	283.35	0.4 ± 0.3^a	nt
Ascorbic acid	170.12	nt	1.15 ± 0.9^b

Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognita

Article Category: Research Paper

Publicado en línea: 17 nov 2022

Páginas: -

Recibido: 31 may 2022

DOI: https://doi.org/10.2478/jofnem-2022-0050

Palabras clave
DPPH, isothiocyanates, raphasatin

© 2022 Aissani and Sebai. published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Figure 2

Correlation between nematicidal and antioxidant activity of the tested compounds (n = 3).

Nematicidal effect of Raphasatin from Raphanus sativus against Meloidogyne incognita

Article Category: Research Paper

Publicado en línea: 17 nov 2022

Páginas: -

Recibido: 31 may 2022

DOI: https://doi.org/10.2478/jofnem-2022-0050

Palabras claveDPPH, isothiocyanates, raphasatin

© 2022 Aissani and Sebai. published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

Figure 2

Correlation between nematicidal and antioxidant activity of the tested compounds (n = 3).

Palabras clave
DPPH, isothiocyanates, raphasatin