Design and simulation of microfluidic device for metabolite screening and quantitative monitoring of drug uptake in cancer cells

1Casavant BP, Berthier E, Theberge AB, Berthier J, Montanez-Sauri SI, Bischel LL, Brakke K, Hedman CJ, Bushman W, Keller NP, Beebe DJ. Suspended microfluidics. Proceedings of the National Academy of Sciences. 2013;110(25):10111-6. https://doi.org/10.1073/pnas.1302566110CasavantBPBerthierEThebergeABBerthierJMontanez-SauriSIBischelLLBrakkeKHedmanCJBushmanWKellerNPBeebeDJ.Suspended microfluidicsProceedings of the National Academy of Sciences201311025101116https://doi.org/10.1073/pnas.130256611010.1073/pnas.1302566110369084823729815Search in Google Scholar

2Carney CM, Muszynski JL, Strotman LN, Lewis SR, O’Connell RL, Beebe DJ, Theberge AB, Jorgensen JS. Cellular microenvironment dictates androgen production by murine fetal Leydig cells in primary culture. Biology of Reproduction. 2014;91(4):1-13. https://doi.org/10.1095/biolreprod.114.118570CarneyCMMuszynskiJLStrotmanLNLewisSRO’ConnellRLBeebeDJThebergeABJorgensenJS.Cellular microenvironment dictates androgen production by murine fetal Leydig cells in primary cultureBiology of Reproduction2014914113https://doi.org/10.1095/biolreprod.114.11857010.1095/biolreprod.114.118570443503025143354Search in Google Scholar

3Theberge AB, Yu J, Young EW, Ricke WA, Bushman W, Beebe DJ. Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesis. Analytical chemistry. 2015;87(6):3239-46. https://doi.org/10.1021/ac503700fThebergeABYuJYoungEWRickeWABushmanWBeebeDJ.Microfluidic multiculture assay to analyze biomolecular signaling in angiogenesisAnalytical chemistry2015876323946https://doi.org/10.1021/ac503700f10.1021/ac503700f440510325719435Search in Google Scholar

4Chen D, Du W, Liu Y, Liu W, Kuznetsov A, Mendez FE, Philipson LH, Ismagilov RF. The chemistrode: a droplet-based microfluidic device for stimulation and recording with high temporal, spatial, and chemical resolution. Proceedings of the National Academy of Sciences. 2008;105(44):16843-8. https://doi.org/10.1073/pnas.0807916105ChenDDuWLiuYLiuWKuznetsovAMendezFEPhilipsonLHIsmagilovRF.The chemistrode: a droplet-based microfluidic device for stimulation and recording with high temporal, spatial, and chemical resolutionProceedings of the National Academy of Sciences200810544168438https://doi.org/10.1073/pnas.080791610510.1073/pnas.0807916105257934118974218Search in Google Scholar

5Liu W, Chen D, Du W, Nichols KP, Ismagilov RF. SlipChip for immunoassays in nanoliter volumes. Analytical chemistry. 2010;82(8):3276-82. https://doi.org/10.1021/ac100044cLiuWChenDDuWNicholsKPIsmagilovRF.SlipChip for immunoassays in nanoliter volumesAnalytical chemistry2010828327682https://doi.org/10.1021/ac100044c10.1021/ac100044c288584220334360Search in Google Scholar

6Shen F, Du W, Kreutz JE, Fok A, Ismagilov RF. Digital PCR on a SlipChip. Lab on a Chip. 2010;10(20):2666-72. https://doi.org/10.1039/c004521gShenFDuWKreutzJEFokAIsmagilovRF.Digital PCR on a SlipChipLab on a Chip20101020266672https://doi.org/10.1039/c004521g10.1039/c004521g294806320596567Search in Google Scholar

7Brouzes E, Medkova M, Savenelli N, Marran D, Twardowski M, Hutchison JB, Rothberg JM, Link DR, Perrimon N, Samuels ML. Droplet microfluidic technology for single-cell high-throughput screening. Proceedings of the National Academy of Sciences. 2009;106(34):14195-200. https://doi.org/10.1073/pnas.0903542106BrouzesEMedkovaMSavenelliNMarranDTwardowskiMHutchisonJBRothbergJMLinkDRPerrimonNSamuelsML.Droplet microfluidic technology for single-cell high-throughput screeningProceedings of the National Academy of Sciences20091063414195200https://doi.org/10.1073/pnas.090354210610.1073/pnas.0903542106273288219617544Search in Google Scholar

8Kiss MM, Ortoleva-Donnelly L, Beer NR, Warner J, Bailey CG, Colston BW, Rothberg JM, Link DR, Leamon JH. High-throughput quantitative polymerase chain reaction in picoliter droplets. Analytical chemistry. 2008;80(23):8975-81. https://doi.org/10.1021/ac801276cKissMMOrtoleva-DonnellyLBeerNRWarnerJBaileyCGColstonBWRothbergJMLinkDRLeamonJH.High-throughput quantitative polymerase chain reaction in picoliter dropletsAnalytical chemistry20088023897581https://doi.org/10.1021/ac801276c10.1021/ac801276c277188419551929Search in Google Scholar

9Mary P, Studer V, Tabeling P. Microfluidic droplet-based liquid- liquid extraction. Analytical chemistry. 2008;80(8):2680-7. https://doi.org/10.1021/ac800088sMaryPStuderVTabelingP.Microfluidic droplet-based liquid- liquid extractionAnalytical chemistry200880826807https://doi.org/10.1021/ac800088s10.1021/ac800088s18351786Search in Google Scholar

10Agresti JJ, Antipov E, Abate AR, Ahn K, Rowat AC, Baret JC, Marquez M, Klibanov AM, Griffiths AD, Weitz DA. Ultrahigh-throughput screening in drop-based microfluidics for directed evolution. Proceedings of the National Academy of Sciences. 2010;107(9):4004-9. https://doi.org/10.1073/pnas.0910781107AgrestiJJAntipovEAbateARAhnKRowatACBaretJCMarquezMKlibanovAMGriffithsADWeitzDA.Ultrahigh-throughput screening in drop-based microfluidics for directed evolutionProceedings of the National Academy of Sciences2010107940049https://doi.org/10.1073/pnas.091078110710.1073/pnas.0910781107284009520142500Search in Google Scholar

11Multiphysics CO, Comsol D, Books CM, Blog CO. Multiphysics Modeling, Finite Element Analysis, and Engineering Simulation Software.MultiphysicsCOComsolDBooksCMBlogCO.Multiphysics Modeling, Finite Element Analysis, and Engineering Simulation SoftwareSearch in Google Scholar

12Website: https://www.zauba.com/import-pgmea-hs-code.html (Accessed 14 March 2017).Website: https://www.zauba.com/import-pgmea-hs-code.html(Accessed 14 March 2017)Search in Google Scholar

13Website: http://profilab24.com/witeg-Wisd-Oven-Gravity-Convection-type-WON (Accessed 14 March 2017).Website: http://profilab24.com/witeg-Wisd-Oven-Gravity-Convection-type-WON (Accessed 14 March 2017)Search in Google Scholar

14Website: http://www.ebay.com/sch/Vacuum-/46548/i.html (Accessed 15 March 2017).Website: http://www.ebay.com/sch/Vacuum-/46548/i.html (Accessed 15 March 2017)Search in Google Scholar

15Website: http://www.ellsworth.com/products/by-market/consumer-products/encapsulants/silicone/dow-corning-sylgard-184-silicone-encapsulant-clear-0.5-kg-kit/ (Accessed 15 March 2017).Website: http://www.ellsworth.com/products/by-market/consumer-products/encapsulants/silicone/dow-corning-sylgard-184-silicone-encapsulant-clear-0.5-kg-kit/ (Accessed 15 March 2017)Search in Google Scholar