<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_jccm-2019-0018_s_006_w2aab3b7b6b1b6b1aab1c18b1Aa"><h3>Introduction</h3><p>In an attempt to identify patients who have successfully survived a resuscitated cardiac arrest (CA), attention is drawn to resistin and S100B protein, two biomarkers that have been studied in relation to CA.</p></sec><sec id="j_jccm-2019-0018_s_007_w2aab3b7b6b1b6b1aab1c18b2Aa"><h3>Aim</h3><p>The study aimed to identify the potential cut-off serum values for resistin and S100B in patients who had CA, compared to healthy volunteers, given that, currently, none of the markers have normal and pathological reference range limits for human assay levels related to this pathology.</p></sec><sec id="j_jccm-2019-0018_s_008_w2aab3b7b6b1b6b1aab1c18b3Aa"><h3>Materials and Methods</h3><p>Forty patients, resuscitated after out-of-hospital CA and forty healthy controls, were included in the study. All patients were followed up for seventy-two hours after CA or until death. Blood samples for biomarkers were collected on admission to the ED (0-time interval) and at 6, 12, 24, 48 and 72 hours following resuscitation. Only one blood sample was collected from the controls. The serum concentrations of biomarkers were measured.</p></sec><sec id="j_jccm-2019-0018_s_009_w2aab3b7b6b1b6b1aab1c18b4Aa"><h3>Results</h3><p>For each time interval, median serum levels of resistin and S100 B were ​ significantly higher in patients with CA compared to healthy controls. The cut-of value for resistin in patients with CA, at the 12-hours versus controls, was > 8.2 ng/ml. The cut-of value for S100B in patients with CA versus controls recorded at 6 hours, was > 11.6 pg/ml.</p></sec><sec id="j_jccm-2019-0018_s_010_w2aab3b7b6b1b6b1aab1c18b5Aa"><h3>Conclusion</h3><p>Serum levels of resistin and S100B are higher among resuscitated CA patients compared to controls.</p></sec></abstract>

IntroductionIn an attempt to identify patients who have successfully survived a resuscitated cardiac arrest (CA), attention is drawn to resistin and S100B protein, two biomarkers that have been studied in relation to CA.AimThe study aimed to identify the potential cut-off serum values for resistin and S100B in patients who had CA, compared to healthy volunteers, given that, currently, none of the markers have normal and pathological reference range limits for human assay levels related to this pathology.Materials and MethodsForty patients, resuscitated after out-of-hospital CA and forty healthy controls, were included in the study. All patients were followed up for seventy-two hours after CA or until death. Blood samples for biomarkers were collected on admission to the ED (0-time interval) and at 6, 12, 24, 48 and 72 hours following resuscitation. Only one blood sample was collected from the controls. The serum concentrations of biomarkers were measured.ResultsFor each time interval, median serum levels of resistin and S100 B were ​ significantly higher in patients with CA compared to healthy controls. The cut-of value for resistin in patients with CA, at the 12-hours versus controls, was > 8.2 ng/ml. The cut-of value for S100B in patients with CA versus controls recorded at 6 hours, was > 11.6 pg/ml.ConclusionSerum levels of resistin and S100B are higher among resuscitated CA patients compared to controls.

Determination of Cut-off Serum Values for Resistin and S100B Protein in Patients Who Survived a Cardiac Arrest

The Journal of Critical Care Medicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

IntroductionIn an attempt to identify patients who have successfully survived a resuscitated cardiac arrest (CA), attention is drawn to resistin and S100B...

Parameters	Time intervals (hours)	Group A*	Group B *	p -values
Resistin	0	7.15 (4.67 to 11.85)	4.10 (2.50 to 5.75)	< 0.001
ng/ml	6	9.85 (4.47 to 17.75)		< 0.001
	12	13.55 (5.55 to 21.02)		< 0.001
	24	12.30 (6.77 to 21.00)		< 0.001
	48	7.25 (3.57 to 14.65)		0.004
	72	7.40 (3.6 to 11.90)		0.002

S100B	0	25.85 (7.07 to 102.52)	5.45 (4.00 to 8.47)	< 0.001
pg/ml	6	17.45 (5.60 to 137.10)		< 0.001
	12	19.70 (4.25 to 127.02)		0.001
	24	15.20 (5.60 to 134.62)		< 0.001
	48	14.10 (6.10 to 79.65)		0.001
	72	8.90 (3.80 to 36.55)		0.05

S100B	AUC (CI95%)	Cut-of pg/ml	Sensitivity (CI 95%)	Specificity (CI 95%)	p
0 hours	0.812 (0.709 to 0.891)	> 8.9	75.00 (58.8 - 87.3)	85.00 (70.2 - 94.3)	<0.001
6 hours	0.769 (0.661 to 0.856)	> 11.6	65.00 (48.3 - 79.4)	92.50 (79.6 - 98.4)	<0.001
12 hours	0.723 (0.612 to 0.818)	> 11.6	57.50 (40.9 - 73.0)	92.50 (79.6 - 98.4)	<0.001

Resistin	AUC (CI95%)	Cut-of ng/ml	Sensitivity (CI 95%)	Specificity (CI 95%)	p
0 hours	0.762 (0.653 to 0.850)	> 5.5	72.50 (56.1 – 84.5)	72.50 (56.1 – 84.5)	<0.001
6 hours	0.807 (0.703 to 0.887)	> 7.6	62.50 (45.8 – 77.3)	95.00 (83.1 – 99.4)	<0.001
12 hours	0.822 (0.720 to 0.898)	> 8.2	70.00 (53.5 – 83.4)	97.50 (86.8 – 99.9)	<0.001

Determination of Cut-off Serum Values for Resistin and S100B Protein in Patients Who Survived a Cardiac Arrest

Article Category: Research Article

Publicado en línea: 09 ago 2019

Páginas: 97 - 102

Recibido: 18 may 2019

Aceptado: 30 jul 2019

DOI: https://doi.org/10.2478/jccm-2019-0018

Palabras clave
resistin, S100B protein, biomarkers, cardiac arrest

© 2019 Raluca M. Tat, Adela Golea, Ştefan C. Vesa, Daniela Ionescu, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Fig. 1

Fig. 2

Serum levels of biomarkers in the first three days, a comparison between Group A and Group B

Cut-off values for S100B for the first 3 measurements following inclusion to the study

Cut-off values for resistin for the first 3 measurements following inclusion to the study

Determination of Cut-off Serum Values for Resistin and S100B Protein in Patients Who Survived a Cardiac Arrest

Article Category: Research Article

Publicado en línea: 09 ago 2019

Páginas: 97 - 102

Recibido: 18 may 2019

Aceptado: 30 jul 2019

DOI: https://doi.org/10.2478/jccm-2019-0018

Palabras claveresistin, S100B protein, biomarkers, cardiac arrest

© 2019 Raluca M. Tat, Adela Golea, Ştefan C. Vesa, Daniela Ionescu, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Fig. 1

Fig. 2

Serum levels of biomarkers in the first three days, a comparison between Group A and Group B

Cut-off values for S100B for the first 3 measurements following inclusion to the study

Cut-off values ​for resistin for the first 3 measurements following inclusion to the study

Palabras clave
resistin, S100B protein, biomarkers, cardiac arrest

Cut-off values for resistin for the first 3 measurements following inclusion to the study