<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>In this study, we conducted a six-month survey to evaluate the current status of three common honey bee viruses (black queen cell virus [BQCV], acute bee paralysis virus [ABPV], and sacbrood virus [SBV]) in Turkey’s Bingöl region and revealed their phylogenetic relationships with the same types of viruses in other parts of the world. We randomly sampled 384 worker honey bees from a total of 128 apiaries from different locations of this region. Molecular tests confirmed the presence of SBV and BQCV, with infection rates of 7.03% and 11.7%, respectively, but ABPV could not be detected in any of the surveyed apiaries. In addition, double infection was detected in an apiary with an infection rate of 0.78%. The sequences of a partial polyprotein gene region of a randomly selected isolate from each detected virus were collected and registered in GenBank under the accession numbers MZ357971 and MZ357972 for SBV and MZ357974 for BQCV. The nucleotide sequence similarity of Turkish BQCV and SBV isolates was 75.71–96.58% and 85.96–92.98%, respectively. A comparison of the phylogenetic tree of Bingöl honey bee viral genomes with other isolates from around the world revealed that Bingöl SBV isolates were closely related to another Turkey isolate while Bingöl BQCV isolate to France, Italy, Australia, and Brazil isolates. To our knowledge, the presence and phylogenetic affinity of SBV and BQCV viruses detected in the present study is the first recording for Turkey’s Bingöl province.</p>
</abstract>

In this study, we conducted a six-month survey to evaluate the current status of three common honey bee viruses (black queen cell virus [BQCV], acute bee paralysis virus [ABPV], and sacbrood virus [SBV]) in Turkey’s Bingöl region and revealed their phylogenetic relationships with the same types of viruses in other parts of the world. We randomly sampled 384 worker honey bees from a total of 128 apiaries from different locations of this region. Molecular tests confirmed the presence of SBV and BQCV, with infection rates of 7.03% and 11.7%, respectively, but ABPV could not be detected in any of the surveyed apiaries. In addition, double infection was detected in an apiary with an infection rate of 0.78%. The sequences of a partial polyprotein gene region of a randomly selected isolate from each detected virus were collected and registered in GenBank under the accession numbers MZ357971 and MZ357972 for SBV and MZ357974 for BQCV. The nucleotide sequence similarity of Turkish BQCV and SBV isolates was 75.71–96.58% and 85.96–92.98%, respectively. A comparison of the phylogenetic tree of Bingöl honey bee viral genomes with other isolates from around the world revealed that Bingöl SBV isolates were closely related to another Turkey isolate while Bingöl BQCV isolate to France, Italy, Australia, and Brazil isolates. To our knowledge, the presence and phylogenetic affinity of SBV and BQCV viruses detected in the present study is the first recording for Turkey’s Bingöl province.

Occurrence and Molecular Phylogeny of Economically Relevant Viruses Infecting Honey Bees (<italic>Apis mellifera</italic> L.) of Bingöl Province, Turkey

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Occurrence and Molecular Phylogeny of Economically Relevant Viruses Infecting Honey Bees ( L.) of Bingöl Province, Turkey

In this study, we conducted a six-month survey to evaluate the current status of three common honey bee viruses (black queen cell virus [BQCV], acute bee...

Virus	Genom specific primerlers (5′→3′)	Amplicon	Gene region	Reference
BQCV	F-TGGTCAGCTCCCACTACCTTAAACR-GCAACAAGAAGAAACGTAAACCAC	700 bp	Polyprotein	Benjeddou et al., 2001
SBV	F-CGTAATTGCGGAGTGGAAAGATTR-AGATTCCTTCGAGGGTACCTCATC	342 bp	Polyprotein	Sguazza et al., 2013
ABPV	F-TTATGTGTCCAGAGACTGTATCCA-GCTCCTATTGCTCGGTTTTTCGGT	900 bp	Coat Protein	Benjeddou et al., 2001

Locations	Number of apiaries	Positivity	Infection incidence
		SBV	BQCV	SBV	BQCV
1	44	4	1	9.1	2.27
2	19	0	0	0	0
3	17	1	4	5.8	23.5
4	12	1	0	8.3	0
5	10	0	0	0	0
6	11	1	4	9.1	45.4
7	4	0	2	0	50.0
8	11	2	4	18.2	36.36
Total	128	9	15	7.03%	11.7%

Occurrence and Molecular Phylogeny of Economically Relevant Viruses Infecting Honey Bees (Apis mellifera L.) of Bingöl Province, Turkey

Article Category: Original Article

Publicado en línea: 22 jun 2022

Páginas: 85 - 96

Recibido: 23 sept 2021

Aceptado: 24 feb 2022

DOI: https://doi.org/10.2478/jas-2022-0006

Palabras clave
ABPV, BQCV, molecular characterization, SBV

© 2022 Abdullah Güller et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1

Fig. 2

Fig. 3

Oligonucleotide primer sets used in this study in order to specify honeybee viruses

Chart showing surveyed apiaries and SBV and BQCV positivity in Bingöl province, Turkey

Occurrence and Molecular Phylogeny of Economically Relevant Viruses Infecting Honey Bees (Apis mellifera L.) of Bingöl Province, Turkey

Article Category: Original Article

Publicado en línea: 22 jun 2022

Páginas: 85 - 96

Recibido: 23 sept 2021

Aceptado: 24 feb 2022

DOI: https://doi.org/10.2478/jas-2022-0006

Palabras claveABPV, BQCV, molecular characterization, SBV

© 2022 Abdullah Güller et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1

Fig. 2

Fig. 3

Oligonucleotide primer sets used in this study in order to specify honeybee viruses

Chart showing surveyed apiaries and SBV and BQCV positivity in Bingöl province, Turkey

Palabras clave
ABPV, BQCV, molecular characterization, SBV