<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The aim of the present study was to evaluate local irritant effects to rabbit skin following a single application of test samples of non-sterile polyamide non-absorbable surgical sutures POLYMED ®. The polar and nonpolar extracts were prepared by using saline solution and olive oil, respectively, after sinking the materials tested (2.0 g) in 10 ml of the corresponding liquid. Incubation was carried out at the temperature of 37°C for 72 h. The saline solution and pure olive oil, which had no contact with the materials tested, were used as negative control samples and were incubated under the same conditions as above. Assessments of the extracts from each material were conducted on 2 albino rabbits of the New Zealand breed. On the back of each animal, 5 intracutaneous injections of the extract tested and 5 injections of the control solution, each of 0.2 ml, were carried out. The degree of irritation was scored at 4, 24, 48, 72 hours after injection and no skin changes were found. The intracutaneous irritation index (III) was calculated and yielded 0.0. Hence it was concluded that under the experimental conditions the extracts of the material tested, i.e. non-sterile polyamide non-absorbable surgical sutures POLYMED ®, were ‘non-irritant’ to the skin of rabbits when compared with the respective control groups. The experimental procedure was conducted according to ISO10993-10.</p></abstract>

The aim of the present study was to evaluate local irritant effects to rabbit skin following a single application of test samples of non-sterile polyamide non-absorbable surgical sutures POLYMED ®. The polar and nonpolar extracts were prepared by using saline solution and olive oil, respectively, after sinking the materials tested (2.0 g) in 10 ml of the corresponding liquid. Incubation was carried out at the temperature of 37°C for 72 h. The saline solution and pure olive oil, which had no contact with the materials tested, were used as negative control samples and were incubated under the same conditions as above. Assessments of the extracts from each material were conducted on 2 albino rabbits of the New Zealand breed. On the back of each animal, 5 intracutaneous injections of the extract tested and 5 injections of the control solution, each of 0.2 ml, were carried out. The degree of irritation was scored at 4, 24, 48, 72 hours after injection and no skin changes were found. The intracutaneous irritation index (III) was calculated and yielded 0.0. Hence it was concluded that under the experimental conditions the extracts of the material tested, i.e. non-sterile polyamide non-absorbable surgical sutures POLYMED ®, were ‘non-irritant’ to the skin of rabbits when compared with the respective control groups. The experimental procedure was conducted according to ISO10993-10.

Assessment of surgical sutures Polymed<sup>®</sup> by intracutaneous irritation test in rabbits

Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University Dunav str. no. 2; Sofia, Bulgaria tel.: +359 2 9236 548, fax +359 2 987 987 4

Medical University, Faculty of Pharmacy, Department of Pharmacology, Pharmacotherapy and Toxicology, Sofia, Bulgaria

Interdisciplinary Toxicology

Assessment of surgical sutures Polymed by intracutaneous irritation test in rabbits

The aim of the present study was to evaluate local irritant effects to rabbit skin following a single application of test samples of non-sterile polyamide...

Assessment of surgical sutures Polymed^® by intracutaneous irritation test in rabbits

Publicado en línea: 24 ago 2013

Páginas: 99 - 102

DOI: https://doi.org/10.2478/intox-2013-0017

This content is open access.

Assessment of surgical sutures Polymed® by intracutaneous irritation test in rabbits

Publicado en línea: 24 ago 2013

Páginas: 99 - 102

DOI: https://doi.org/10.2478/intox-2013-0017

This content is open access.

Assessment of surgical sutures Polymed^® by intracutaneous irritation test in rabbits