Research on real-time nucleic acid detection device based on microfluidic technology

In recent years, the point-of-care testing (POCT) device for nucleic acid detection has received extensive attention. POCT device provides rapid, high sensitivity and accuracy of on-site diagnosis and can quantify the nucleic acid in clinical samples in real time. The integration of microfluidics ensures accurate fluid control, minimizes sample volume, and improves detection efficiency. In addition, POCT devices can conduct multichannel analysis, facilitate continuous monitoring, and provide comprehensive quantitative data. Due to its portability, cost-effectiveness, and ease of use, POCT device represents a promising solution for extensive, reliable, and timely nucleic acid detection in various medical environments. This review summarizes the research and development of nucleic acid detection POCT devices based on microfluidic technology in recent years, and the development trend of the device has prospected.