<abstract xmlns="http://www.w3.org/1999/xhtml"><p>In spaceflight experiments, model organisms are used to assess the effects of microgravity on specific biological systems. In many cases, only one biological system is of interest to the Principal Investigator. To maximize the scientific return of experiments, the remaining spaceflight tissue is categorized, documented, and stored in the biobank at NASA Ames Research Center, which is maintained by the Ames Life Science Data Archive (ALSDA). The purpose of this study is to evaluate the state of a sample set of tissues from the ALSDA biobank. Garnering information – such as downstream functional analysis for the generation of omics datasets – from tissues is, in part, dependent on the state of sample preservation. RNA integrity number (RIN) values have been calculated for rodent liver tissues that were part of scientific payloads returned from the International Space Station (ISS). Rat livers from Spacelab Life Sciences 1 (SLS-1) and mouse livers from Commercial Biomedical Test Module 3 (CBTM-3), Rodent Research 1 (RR1), and Rodent Research 3 (RR3) were tested. It was found that mean RIN values from CBTM-3, RR1, and RR3 were suitable for downstream functional analysis (RIN > 5) while the mean RIN value for SLS-1 was not (RIN = 2.5 ± 0.1). Information from this study lays the foundation for future efforts in determining the types of assays that are most appropriate for different tissues in the ALSDA biobank and similar preservation facilities, which would aid in shaping the design of experiments.</p></abstract>

In spaceflight experiments, model organisms are used to assess the effects of microgravity on specific biological systems. In many cases, only one biological system is of interest to the Principal Investigator. To maximize the scientific return of experiments, the remaining spaceflight tissue is categorized, documented, and stored in the biobank at NASA Ames Research Center, which is maintained by the Ames Life Science Data Archive (ALSDA). The purpose of this study is to evaluate the state of a sample set of tissues from the ALSDA biobank. Garnering information – such as downstream functional analysis for the generation of omics datasets – from tissues is, in part, dependent on the state of sample preservation. RNA integrity number (RIN) values have been calculated for rodent liver tissues that were part of scientific payloads returned from the International Space Station (ISS). Rat livers from Spacelab Life Sciences 1 (SLS-1) and mouse livers from Commercial Biomedical Test Module 3 (CBTM-3), Rodent Research 1 (RR1), and Rodent Research 3 (RR3) were tested. It was found that mean RIN values from CBTM-3, RR1, and RR3 were suitable for downstream functional analysis (RIN > 5) while the mean RIN value for SLS-1 was not (RIN = 2.5 ± 0.1). Information from this study lays the foundation for future efforts in determining the types of assays that are most appropriate for different tissues in the ALSDA biobank and similar preservation facilities, which would aid in shaping the design of experiments.

Temporal RNA Integrity Analysis of Archived Spaceflight Biological Samples

Volunteer Internship Program, National Aeronautics and Space Administration (NASA) Ames Research Center

National Aeronautics and Space Administration (NASA) Ames Research Center

Gravitational and Space Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

{"article-title":"Temporal RNA Integrity Analysis of Archived Spaceflight Biological Samples"}

In spaceflight experiments, model organisms are used to assess the effects of microgravity on specific biological systems. In many cases, only one biological...

Temporal RNA Integrity Analysis of Archived Spaceflight Biological Samples

Article Category: Research Article

Publicado en línea: 21 jul 2020

Páginas: 44 - 53

DOI: https://doi.org/10.2478/gsr-2018-0009

Palabras clave
RNA Integrity Number, Spaceflight Tissues, Biobanking

© 2018 Elizabeth Delgadillo Talburt et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Temporal RNA Integrity Analysis of Archived Spaceflight Biological Samples

Article Category: Research Article

Publicado en línea: 21 jul 2020

Páginas: 44 - 53

DOI: https://doi.org/10.2478/gsr-2018-0009

Palabras claveRNA Integrity Number, Spaceflight Tissues, Biobanking

© 2018 Elizabeth Delgadillo Talburt et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
RNA Integrity Number, Spaceflight Tissues, Biobanking