<abstract xmlns="http://www.w3.org/1999/xhtml"><p>Suborbital spaceflights, carrying scientific payloads, allow scientists not only to test the feasibility of their payloads, but they also provide the basis for refining scientific hypotheses to be later tested on the International Space Station (ISS). Therefore, it is essential to establish robust pre-flight procedures in order to take advantage of this unique research platform to facilitate payload delivery. In the present study, we assessed zebrafish larvae behavior as a precursor for the future suborbital spaceflight involving research on the musculoskeletal system. Zebrafish larvae were exposed to the same physiological stressors they would encounter during suborbital spaceflight: alterations in light, thermal, and centrifugation conditions. Their behavioral responses were analyzed using the DanioVision (Noldus) behavioral tracking system. Our results showed that zebrafish were most active when kept in a dark environment as measured by swim distance. Also, thermal alterations revealed that zebrafish larvae adapted well to the different temperatures ranging from 25°C to 32°C with the highest levels of locomotor activity observed at 32°C. Finally, the centrifugation tests demonstrated that although zebrafish were exhausted initially, their recovery process was short, lasting for approximately five minutes. Taken together, our findings support the hypothesis that using zebrafish larvae is a feasible model for future suborbital flights. Thus, the lessons learned allow us to propel this research with more refined and realistic procedures as a precursor for orbital flights to the ISS and to cislunar space.</p></abstract>

Suborbital spaceflights, carrying scientific payloads, allow scientists not only to test the feasibility of their payloads, but they also provide the basis for refining scientific hypotheses to be later tested on the International Space Station (ISS). Therefore, it is essential to establish robust pre-flight procedures in order to take advantage of this unique research platform to facilitate payload delivery. In the present study, we assessed zebrafish larvae behavior as a precursor for the future suborbital spaceflight involving research on the musculoskeletal system. Zebrafish larvae were exposed to the same physiological stressors they would encounter during suborbital spaceflight: alterations in light, thermal, and centrifugation conditions. Their behavioral responses were analyzed using the DanioVision (Noldus) behavioral tracking system. Our results showed that zebrafish were most active when kept in a dark environment as measured by swim distance. Also, thermal alterations revealed that zebrafish larvae adapted well to the different temperatures ranging from 25°C to 32°C with the highest levels of locomotor activity observed at 32°C. Finally, the centrifugation tests demonstrated that although zebrafish were exhausted initially, their recovery process was short, lasting for approximately five minutes. Taken together, our findings support the hypothesis that using zebrafish larvae is a feasible model for future suborbital flights. Thus, the lessons learned allow us to propel this research with more refined and realistic procedures as a precursor for orbital flights to the ISS and to cislunar space.

Investigation of Zebrafish Larvae Behavior as Precursor for Suborbital Flights: Feasibility Study

University of Texas Health Science Center

Department of Drug Discovery and Biomedical Sciences

Gravitational and Space Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Suborbital spaceflights, carrying scientific payloads, allow scientists not only to test the feasibility of their payloads, but they also provide the basis for...

Investigation of Zebrafish Larvae Behavior as Precursor for Suborbital Flights: Feasibility Study

Article Category: Research Article

Publicado en línea: 20 jul 2020

Páginas: 37 - 57

DOI: https://doi.org/10.2478/gsr-2018-0004

Palabras clave
Zebrafish Larvae Locomotor Motion, Suborbital, Physiological Stressors, Muscle Atrophy

© 2018 Pedro J. Llanos et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Investigation of Zebrafish Larvae Behavior as Precursor for Suborbital Flights: Feasibility Study

Article Category: Research Article

Publicado en línea: 20 jul 2020

Páginas: 37 - 57

DOI: https://doi.org/10.2478/gsr-2018-0004

Palabras claveZebrafish Larvae Locomotor Motion, Suborbital, Physiological Stressors, Muscle Atrophy

© 2018 Pedro J. Llanos et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
Zebrafish Larvae Locomotor Motion, Suborbital, Physiological Stressors, Muscle Atrophy