<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Hepatitis E virus (HEV) represents the causative agent of acute viral hepatitis in the world. The virus belongs to the genus <italic>Paslahepevirus</italic>, species <italic>Paslahepevirus balayani</italic>, which contains 8 different genotypes and at least 14 reference HEV-3 subtypes. Recently we have identified three isolates in a Slovakian pig farm as a new unclassified HEV-3 subtype. The aim of this study was to sequence and analyze additional regions of HEV genome for PER5 isolate, a representative isolate of potential new HEV-3 subtype. The phylogenetic analyses of 749 bp and 828 bp DNA fragments amplified from ORF1 revealed that both sequences fall outside two major clades (3abchij and 3efg) of the HEV-3 genotype as well as the other HEV-3 subtypes. The nucleotide identity with other HEV-3 subtypes indicated low values within the range 79.2–84.1 % as well. The results provide further support for the circulation of a new HEV-3 subtype in the pig farm in Slovakia.</p>
</abstract>

Hepatitis E virus (HEV) represents the causative agent of acute viral hepatitis in the world. The virus belongs to the genus Paslahepevirus, species Paslahepevirus balayani, which contains 8 different genotypes and at least 14 reference HEV-3 subtypes. Recently we have identified three isolates in a Slovakian pig farm as a new unclassified HEV-3 subtype. The aim of this study was to sequence and analyze additional regions of HEV genome for PER5 isolate, a representative isolate of potential new HEV-3 subtype. The phylogenetic analyses of 749 bp and 828 bp DNA fragments amplified from ORF1 revealed that both sequences fall outside two major clades (3abchij and 3efg) of the HEV-3 genotype as well as the other HEV-3 subtypes. The nucleotide identity with other HEV-3 subtypes indicated low values within the range 79.2–84.1 % as well. The results provide further support for the circulation of a new HEV-3 subtype in the pig farm in Slovakia.

The Genetic Analysis of Potential New HEV-3 Subtype Identified on Pig Farm in Slovakia

Department of Epizootiology, Parasitology and Protection of One Health

University of Veterinary Medicine and Pharmacy in Košice

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Hepatitis E virus (HEV) represents the causative agent of acute viral hepatitis in the world. The virus belongs to the genus Paslahepevirus, species...

The Genetic Analysis of Potential New HEV-3 Subtype Identified on Pig Farm in Slovakia

Publicado en línea: 19 jun 2023

Páginas: 11 - 17

Recibido: 03 feb 2023

Aceptado: 22 mar 2023

DOI: https://doi.org/10.2478/fv-2023-0012

Palabras clave
domestic pig, hepatitis E, HEV-3, sequencing, subtype

© 2023 Božena Kočíková et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The Genetic Analysis of Potential New HEV-3 Subtype Identified on Pig Farm in Slovakia

Publicado en línea: 19 jun 2023

Páginas: 11 - 17

Recibido: 03 feb 2023

Aceptado: 22 mar 2023

DOI: https://doi.org/10.2478/fv-2023-0012

Palabras clavedomestic pig, hepatitis E, HEV-3, sequencing, subtype

© 2023 Božena Kočíková et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
domestic pig, hepatitis E, HEV-3, sequencing, subtype