<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The goal of this paper was to study the effect of <italic>Lactobacillus reuteri</italic> B1/1, B2/1 and B6/1 on the relative expression of selected interleukins (IL-1β, IL-15), macrophage inflammatory protein (MIP-1β), and the relative percentage of T lymphocyte subpopulations in peripheral mononuclear blood cells (PMBCs). The mRNA expression levels of interleukins and MIP-1β of PMBCs were evaluated at 24 h and 48 h post inoculation using the quantitative real-time polymerase chain reaction (qRT-PCR). The percentage of T lymphocyte subpopulations in PMBCs was determined by flow cytometry. The group that was administered <italic>L. reuteri</italic> B1/1 had the most significant stimulation of the expression of pro-inflammatory interleukins and MIP-1β, in particular after 24 h. Similarly, we observed a rise in the relative percentage of T cells including CD3+, CD4+ and CD8+ lymphocytes in the groups with <italic>L. reuteri</italic> B1/1 and <italic>L. reuteri</italic> B2/1. Overall, <italic>L. reuteri</italic> B1/1 and <italic>L. reuteri</italic> B2/1 showed a promising stimulatory effect on the relative expression of pro-inflammatory interleukins, MIP-1β and percentage of T cell subpopulations <italic>in vitro</italic>. On the flip side, <italic>L. reuteri</italic> B6/1 did not induce the expression of the IL-1β gene.</p></abstract>

The goal of this paper was to study the effect of Lactobacillus reuteri B1/1, B2/1 and B6/1 on the relative expression of selected interleukins (IL-1β, IL-15), macrophage inflammatory protein (MIP-1β), and the relative percentage of T lymphocyte subpopulations in peripheral mononuclear blood cells (PMBCs). The mRNA expression levels of interleukins and MIP-1β of PMBCs were evaluated at 24 h and 48 h post inoculation using the quantitative real-time polymerase chain reaction (qRT-PCR). The percentage of T lymphocyte subpopulations in PMBCs was determined by flow cytometry. The group that was administered L. reuteri B1/1 had the most significant stimulation of the expression of pro-inflammatory interleukins and MIP-1β, in particular after 24 h. Similarly, we observed a rise in the relative percentage of T cells including CD3+, CD4+ and CD8+ lymphocytes in the groups with L. reuteri B1/1 and L. reuteri B2/1. Overall, L. reuteri B1/1 and L. reuteri B2/1 showed a promising stimulatory effect on the relative expression of pro-inflammatory interleukins, MIP-1β and percentage of T cell subpopulations in vitro. On the flip side, L. reuteri B6/1 did not induce the expression of the IL-1β gene.

<italic>In Vitro</italic> Study of Immune Properties of New Lactobacilli Isolates from Pheasant Gut

Department of Microbiology and Immunology

University of Veterinary Medicine and Pharmacy in Košice

Folia Veterinaria

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

 Study of Immune Properties of New Lactobacilli Isolates from Pheasant Gut

The goal of this paper was to study the effect of Lactobacillus reuteri B1/1, B2/1 and B6/1 on the relative expression of selected interleukins (IL-1β, IL-15),...

In Vitro Study of Immune Properties of New Lactobacilli Isolates from Pheasant Gut

Publicado en línea: 13 abr 2020

Páginas: 39 - 47

Recibido: 13 dic 2019

Aceptado: 05 feb 2020

DOI: https://doi.org/10.2478/fv-2020-0006

Palabras clave
cytokine, lactobacili, peripheral mononuclear blood cells

© 2020 V. Karaffová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

In Vitro Study of Immune Properties of New Lactobacilli Isolates from Pheasant Gut

Publicado en línea: 13 abr 2020

Páginas: 39 - 47

Recibido: 13 dic 2019

Aceptado: 05 feb 2020

DOI: https://doi.org/10.2478/fv-2020-0006

Palabras clavecytokine, lactobacili, peripheral mononuclear blood cells

© 2020 V. Karaffová et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Palabras clave
cytokine, lactobacili, peripheral mononuclear blood cells