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Effect of foliar application of zinc on annual productivity, foliar nutrients, bioactive compounds and oxidative metabolism in pecan


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Figure 1.

Morphology of the ZnO NP samples determined by scanning (A,B) and transmission (C,D) electron microscopy. ZnO NPs, zinc oxide nanoparticles.
Morphology of the ZnO NP samples determined by scanning (A,B) and transmission (C,D) electron microscopy. ZnO NPs, zinc oxide nanoparticles.

Figure 2.

Elemental analysis (chemical composition) by energy dispersive X-ray scattering (A) and crystalline structure by X-ray diffraction (B) of the sample ZnO NPs (Bautista-Díaz et al., 2021). ZnO NPs, zinc oxide nanoparticles.
Elemental analysis (chemical composition) by energy dispersive X-ray scattering (A) and crystalline structure by X-ray diffraction (B) of the sample ZnO NPs (Bautista-Díaz et al., 2021). ZnO NPs, zinc oxide nanoparticles.

Figure 3.

NSCs in ‘Western Schley’ pecan leaflets sprayed with sulphates, chelates and zinc nanoparticles. The data are means of treatments in 2019 and 2020. Bars with the same letter are not significantly different (Tukey’s test, p ≤ 0.05). Error bars represent standard deviations (n = 10). NSC, Non-structural carbohydrate; NZN, nitrazinc, containing Zn(NO3)2 and urea-ammonium nitrate fertiliser; Zn-EDTA, zinc ethylene diamine-tetra-acetic acid; ZnO NPs, zinc oxide nanoparticles; ZnSO4, zinc sulphate.
NSCs in ‘Western Schley’ pecan leaflets sprayed with sulphates, chelates and zinc nanoparticles. The data are means of treatments in 2019 and 2020. Bars with the same letter are not significantly different (Tukey’s test, p ≤ 0.05). Error bars represent standard deviations (n = 10). NSC, Non-structural carbohydrate; NZN, nitrazinc, containing Zn(NO3)2 and urea-ammonium nitrate fertiliser; Zn-EDTA, zinc ethylene diamine-tetra-acetic acid; ZnO NPs, zinc oxide nanoparticles; ZnSO4, zinc sulphate.

Figure 4.

Yield, nut weight and kernel percentage in ‘Western Schley’ pecan trees with the application of sulphates, chelates and zinc nanoparticles. The data are means of treatments in 2019 and 2020. Bars with the same letter are not significantly different (Tukey’s test, p ≤ 0.05). Error bars represent standard deviations (n = 10). NZN, nitrazinc, containing Zn(NO3)2 and urea-ammonium nitrate fertiliser; Zn-EDTA, zinc ethylene diamine-tetra-acetic acid; ZnO NPs, zinc oxide nanoparticles; ZnSO4, zinc sulphate.
Yield, nut weight and kernel percentage in ‘Western Schley’ pecan trees with the application of sulphates, chelates and zinc nanoparticles. The data are means of treatments in 2019 and 2020. Bars with the same letter are not significantly different (Tukey’s test, p ≤ 0.05). Error bars represent standard deviations (n = 10). NZN, nitrazinc, containing Zn(NO3)2 and urea-ammonium nitrate fertiliser; Zn-EDTA, zinc ethylene diamine-tetra-acetic acid; ZnO NPs, zinc oxide nanoparticles; ZnSO4, zinc sulphate.

Figure 5.

(A) Micronutrient concentrations in ‘Western Schley’ pecan tree leaflets sprayed with sulphates, chelates and zinc nanoparticles. Bars with the same letter are not significantly different (Tukey’s test,p ≤ 0.05). Error bars represent standard deviations (n = 10). NZN, nitrazinc, containing Zn(NO3)2 and urea–ammonium nitrate fertiliser; Zn-EDTA, zinc ethylene diamine-tetra-acetic acid; ZnO NPs, zinc oxide nanoparticles; ZnSO4, zinc sulphate. (B) Micronutrient concentration in ‘Western Schley’ pecan trees with the application of sulphates, chelates and zinc nanoparticles. Bars with the same letter are not significantly different (Tukey’s test, p ≤ 0.05). Error bars represent standard deviations (n = 10). NZN, nitrazinc, containing Zn(NO3)2 and urea-ammonium nitrate fertiliser; Zn-EDTA, zinc ethylene diamine-tetra-acetic acid; ZnO NPs, zinc oxide nanoparticles; ZnSO4, zinc sulphate.
(A) Micronutrient concentrations in ‘Western Schley’ pecan tree leaflets sprayed with sulphates, chelates and zinc nanoparticles. Bars with the same letter are not significantly different (Tukey’s test,p ≤ 0.05). Error bars represent standard deviations (n = 10). NZN, nitrazinc, containing Zn(NO3)2 and urea–ammonium nitrate fertiliser; Zn-EDTA, zinc ethylene diamine-tetra-acetic acid; ZnO NPs, zinc oxide nanoparticles; ZnSO4, zinc sulphate. (B) Micronutrient concentration in ‘Western Schley’ pecan trees with the application of sulphates, chelates and zinc nanoparticles. Bars with the same letter are not significantly different (Tukey’s test, p ≤ 0.05). Error bars represent standard deviations (n = 10). NZN, nitrazinc, containing Zn(NO3)2 and urea-ammonium nitrate fertiliser; Zn-EDTA, zinc ethylene diamine-tetra-acetic acid; ZnO NPs, zinc oxide nanoparticles; ZnSO4, zinc sulphate.

Concentration of photosynthetic pigments and phenolic compounds in ‘Western Schley’ pecan tree leaflets sprayed with sulphates, chelates and zinc nanoparticles.

Treatments (200 mg · L−1) TChl (μg · g−1) TC (μg · g−1) TFl (mg GAE · g−1) TP (mg QE · g−1)
NZN (control) 45.1 a 14.7 a 55.7 a 18.4 a
ZnSO4 45.5 a 10.5 b 53.7 a 17.1 a
Zn-EDTA 41.7 b 10.7 b 61.6 a 20.0 a
ZnO NPs 40.7 b 12.7 ab 63.6 a 22.0 a

Oxidative metabolism and AC in ‘Western Schley’ pecan tree leaflets sprayed with sulphates, chelates and zinc nanoparticles.

Treatments (200 mg · L−1) SOD H2O2 CAT GPx AC
NZN (control) 1.4 a 0.4 a 3.5 a 3.2 b 83.1 a
ZnSO4 1.3 ab 0.3 b 2.3 b 5.6 a 80.6 a
Zn-EDTA 1.2 c 0.4 b 2.3 b 5.2 a 76.9 b
ZnO NPs 1.3 b 0.3 b 2.4 b 5.3 a 82.6 a

Yield, nut weight and kernel percentage in ‘Western Schley’ pecan tree leaflets sprayed with sulphates, chelates and zinc nanoparticles.

Treatments (200 mg · L−1) Nut quality
Yield (kg per tree) Nut weight (g · kg 1 FW) Kernel (%)
2019 2020 2019 2020 2019 2020
NZN (control) 9.9 a 15.6 b 156.1 a 166.1 b 52.2 a 53.0 a
ZnSO4 9.1 a 14.1 b 153.4 a 167.4 b 60.4 a 58.0 a
Zn-EDTA 8.7 a 14.9 b 152.5 a 164.5 b 54.3 a 54.3 a
ZnO NPs 9.9 a 14.5 b 158.6 a 166.6 b 54.9 a 54.5 a

Nutrient concentrations in ‘Western Schley’ pecan tree leaflets sprayed with sulphates, chelates and zinc nanoparticles.

Treatments (200 mg · L−1) Concentration (g · kg−1)
Total-N Total-P K+ Ca2+ Mg2+
2019 2020 2019 2020 2019 2020 2019 2020 2019 2020
NZN (control) 27.9 a 23.9 b 2.7 a 2.0 b 19.7 a 17.6 a 33.66 a 28.32 b 4.8 a 4.1 b
ZnSO4 26.3 a 27.3 a 2.1 a 1.7 b 19.24 a 20.3 a 31.62 a 33.10 a 3.9 a 4.3 a
Zn-EDTA 24.7 a 25.4 a 2.7 a 1.8 b 17.0 a 22.2 b 31.10 a 32.14 a 3.8 a 4.4 a
ZnO NPs 24.3 a 25.9 a 2.5 a 1.9 b 21.4 a 19.4 a 30.30 a 31.14 a 4.3 a 4.1 a
  Concentration (mg · kg−1)  
Fe2+ Cu2+ Mn2+ Zn2+
NZN (control) 139.4 a 181.9 b 6.3 a 5.8 b 231.0 a 212.4 b 48.5 a 45.3 a
ZnSO4 154.9 a 191.6 b 6.4 a 6.2 a 236.8 a 233.8 a 39.6 a 40.4 a
Zn-EDTA 160.8 a 194.5 a 6.5 a 6.3 a 242.4 a 236.0 a 35.8 a 38.0 b
ZnO NPs 118.3 a 179.4 b 6.2 a 6.6 b 227.8 a 241.0 b 39.3 a 35.8 b

Concentration of NSCs in ‘Western Schley’ pecan tree leaflets sprayed with sulphates, chelates and zinc nanoparticles.

Treatments (200 mg · L−1) NSCs (g · kg−1)
Fructose Glucose Sucrose Starch
2019 2020 2019 2020 2019 2020 2019 2020
NZN (control) 37.9 a 34.3 a 41.9 a 38.0 a 41.9 a 43.5 a 38.8 a 40.2 a
ZnSO4 40.7 a 38.5 a 45.0 a 42.6 a 37.5 a 36.7 a 34.6 a 34.5 a
Zn-EDTA 39.3 a 40.3 a 43.5 a 44.6 a 40.8 a 39.4 a 37.5 a 36.6 a
ZnO NPs 39.5 a 38.2 a 43.6 a 42.9 a 36.7 a 39.3 a 35.7 a 39.6 a
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Life Sciences, Plant Science, Zoology, Ecology, other