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Evaluation of the possibility of obtaining viable seeds from the cross-breeding Hippeastrum × chmielii Chm. with selected cultivars of Hippeastrum hybridum Hort.

Przemysław Marciniak
Przemysław Marciniak
, 
Agata Jędrzejuk
Agata Jędrzejuk
 y 
Dariusz Sochacki
Dariusz Sochacki
   | 05 jun 2021
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Article Category: Original Article
Publicado en línea: 05 jun 2021
Páginas: 185 - 194
Recibido: 24 nov 2020
Aceptado: 19 abr 2021
DOI: https://doi.org/10.2478/fhort-2021-0014
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© 2021 Przemysław Marciniak et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Figure 1

Clones of H. × chmielii – No. 6 (left) and No. 18 (right).
Clones of H. × chmielii – No. 6 (left) and No. 18 (right).

Figure 2

Cultivars of H. hybridum tested – ‘Gervase’, ‘Rio Negro’ and ‘Royal Velvet’ (from left to right).
Cultivars of H. hybridum tested – ‘Gervase’, ‘Rio Negro’ and ‘Royal Velvet’ (from left to right).

Figure 3

The receptive stigma of Hippeastrum × chmielii stained by alizarine red – clone No. 6 (A) and clone No. 18 (B).
The receptive stigma of Hippeastrum × chmielii stained by alizarine red – clone No. 6 (A) and clone No. 18 (B).

Figure 4

The receptive ovule of Hippeastrum × chmielii stained by alizarine red – clone No. 6 (A) and clone No. 18 (B).
The receptive ovule of Hippeastrum × chmielii stained by alizarine red – clone No. 6 (A) and clone No. 18 (B).

Figure 5

Pollen grains stained by acetocarmine – H. hybridum ‘Gervase’ (A), H. hybridum ‘Rio Negro’ (B) and H. hybridum ‘Royal Velvet’ (C). White arrows show viable pollen grains; black arrows show non-stainable/non-viable grains.
Pollen grains stained by acetocarmine – H. hybridum ‘Gervase’ (A), H. hybridum ‘Rio Negro’ (B) and H. hybridum ‘Royal Velvet’ (C). White arrows show viable pollen grains; black arrows show non-stainable/non-viable grains.

Figure 6

Sprouting pollen tubes on the germination medium – H. hybridum ‘Rio Negro’ (A) and H. hybridum ‘Royal Velvet’ (B).
Sprouting pollen tubes on the germination medium – H. hybridum ‘Rio Negro’ (A) and H. hybridum ‘Royal Velvet’ (B).

Figure 7

Germinating pollen grains of H. hybridum ‘Rio Negro’ on the stigma (A) and pollen tubes of ‘Rio Negro’ penetrated the ovule (B).
Germinating pollen grains of H. hybridum ‘Rio Negro’ on the stigma (A) and pollen tubes of ‘Rio Negro’ penetrated the ovule (B).

The number of seeds obtained from individual crossings of H. × chmielii with three cultivars.

Crossing Number of flowers Number of collected seeds from one flower (min – max) Average number of collected seeds per flower ± SD Total number of seeds
H. × chmielii 18 × ‘Gervase’ 10 31–76 57.7 ± 15.53 577
H. × chmielii 18 × ‘Royal Velvet’ 15 24–86 59.1 ± 17.99 828
H. × chmielii 18 × ‘Rio Negro’ 17 22–68 43.6 ± 17.68 305
H. × chmielii 6 × ‘Gervase’ 10 25–94 48.6 ± 22.17 340
H. × chmielii 6 × ‘Royal Velvet’ 15 53–83 70.9 ± 9.44 993
H. × chmielii 6 × ‘Rio Negro’ 10 0 0 0
Total number of seeds from all crossings 3,043

Percentage of viable/stainable pollen grains of cultivars H. hybridum – ‘Gervase’; ‘Royal Velvet’; ‘Rio Negro’.

Cultivars Total number of pollen grain ± SD Number of viable pollen grains ± SD Percentage of viable pollen grains (%) ± SD
H. hybridum ‘Gervase’ 195 ± 4.08 162 ± 0.82 83.0 ± 0.06 b*
H. hybridum ‘Royal Velvet’ 177 ± 1.63 145 ± 3.30 82.7 ± 5.51 b
H. hybridum ‘Rio Negro’ 285 ± 4.08 210 ± 1.63 66.4 ± 9.21 a

Percentage of germinated seeds of H. × chmielii pollinated by pollen of three cultivars depending on the germination method.

Crossing Germination on Petri dishes (%)* Germination in water in the jars (%)*
H. × chmielii 18 × ‘Gervase’ 55.0 ± 5.61 a** 71.2 ± 6.77 b
H. × chmielii 18 × ‘Royal Velvet’ 67.2 ± 9.54 a 70.6 ± 8.66 a
H. × chmielii 18 × ‘Rio Negro’ 48.3 ± 6.01 a 58.4 ± 5.80 a
H. × chmielii 6 × ‘Gervase’ 72.7 ± 12.02 a 73.8 ± 10.88 a
H. × chmielii 6 × ‘Royal Velvet’ 75.4 ± 6.28 a 77.9 ± 4.62 a
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