<abstract xmlns="http://www.w3.org/1999/xhtml">In forest production, there is an emerging tendency towards the planting of fast-growing trees as attractive, renewable energy sources. Hence, efforts were made to develop a method of micropropagation by organogenesis of seven clones of black locust (<italic>Robinia pseudoacacia</italic> L.) that are resistant to propagation by traditional vegetative methods, as well as one plus tree (no. 9755) at the age of 60, to see if the age of the mother plant is a limitation in the micropropagation of black locust trees. Overall results suggest that Murashige and Skoog medium supplemented with 30 g l−1 sucrose, 0.6 mg l−1 6-benzylaminopurine (BAP) and 0.1 mg l−1 naphthalene acetic acid (NAA) is better for the propagation of each genotype of <italic>R. pseudoacacia</italic> than Woody Plant Medium with the same growth regulators, and the age of the donor plant does not affect the organogenic potential. Recalcitrance to adventitious rooting from adventitious shoot formation is a major limitation for the clonal micropropagation of forest trees. Our results showed that although the roots were also formed spontaneously in the growth medium without growth hormones for the tested black locust clones, the application of auxin increased the total root length compared to that in the medium with active carbon and control. A significant effect of the additives of hormone and sucrose on the total root length was found. Increasing the sucrose concentration stimulated the induction of roots in each of the tested concentrations (5, 10, 15 or 20 g l−1). Additionally, the change in sugar dose in the rooting medium caused significant differences in total root length.</abstract>

In forest production, there is an emerging tendency towards the planting of fast-growing trees as attractive, renewable energy sources. Hence, efforts were made to develop a method of micropropagation by organogenesis of seven clones of black locust (Robinia pseudoacacia L.) that are resistant to propagation by traditional vegetative methods, as well as one plus tree (no. 9755) at the age of 60, to see if the age of the mother plant is a limitation in the micropropagation of black locust trees. Overall results suggest that Murashige and Skoog medium supplemented with 30 g l−1 sucrose, 0.6 mg l−1 6-benzylaminopurine (BAP) and 0.1 mg l−1 naphthalene acetic acid (NAA) is better for the propagation of each genotype of R. pseudoacacia than Woody Plant Medium with the same growth regulators, and the age of the donor plant does not affect the organogenic potential. Recalcitrance to adventitious rooting from adventitious shoot formation is a major limitation for the clonal micropropagation of forest trees. Our results showed that although the roots were also formed spontaneously in the growth medium without growth hormones for the tested black locust clones, the application of auxin increased the total root length compared to that in the medium with active carbon and control. A significant effect of the additives of hormone and sucrose on the total root length was found. Increasing the sucrose concentration stimulated the induction of roots in each of the tested concentrations (5, 10, 15 or 20 g l−1). Additionally, the change in sugar dose in the rooting medium caused significant differences in total root length.

Micropropagation and <italic>in vitro</italic> rooting of <italic>Robinia pseudoacacia</italic> L. recalcitrant genotypes

Department of Silviculture and Genetics of Forest Trees

Department of Forest Management Planning, Dendrometry and Forest Economics, Laboratory of Dendrometry and Forest Productivity

Folia Forestalia Polonica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Micropropagation and  rooting of  L. recalcitrant genotypes

In forest production, there is an emerging tendency towards the planting of fast-growing trees as attractive, renewable energy sources. Hence, efforts were...

Micropropagation and in vitro rooting of Robinia pseudoacacia L. recalcitrant genotypes

Publicado en línea: 31 mar 2020

Páginas: 13 - 21

Recibido: 04 nov 2019

Aceptado: 29 nov 2019

DOI: https://doi.org/10.2478/ffp-2020-0002

Palabras clave
black locust, cultures, medium effect

© 2020 Iwona Szyp-Borowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Micropropagation and in vitro rooting of Robinia pseudoacacia L. recalcitrant genotypes

Publicado en línea: 31 mar 2020

Páginas: 13 - 21

Recibido: 04 nov 2019

Aceptado: 29 nov 2019

DOI: https://doi.org/10.2478/ffp-2020-0002

Palabras claveblack locust, cultures, medium effect

© 2020 Iwona Szyp-Borowska et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Palabras clave
black locust, cultures, medium effect