<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Objective.</bold> The cytochrome <italic>11B2</italic> aldosterone synthase gene (<italic>CYP11B2</italic>) that links to aldosterone synthase enzyme synthesis changes and blood pressure regulation is of particular interest among the renin-angiotensin-aldosterone system encoding genes.<bold>Methods.</bold> One-hundred hypertensive patients with target-organ damaging (2nd stage), moderate, high or very high cardiovascular risk were involved in the case-control study. Mean age was 59.87±8.02 years. Diabetes Mellitus type 2 (DM2) was in 28 persons. Chronic kidney disease (CKD) was diagnosed in 29 persons according to the National Kidney Foundation recommendations (2012) after glomerular filtration rate (GFR) decline &lt;60 ml/min/1.73m2 for ≥3 months (measured by CKD-EPI equations). Aldosterone, cystatin-C, and creatinine levels were measured in serum. Control group included 48 practically healthy persons of relevant age. Gene’s nucleotide polymorphism <italic>CYP11B2</italic> (-344C/T) was examined by polymerase chain reaction.<bold>Results.</bold> CKD evolution in hypertensive patients followed by higher systolic and diastolic blood pressure (SBP, DBP) values increased creatinine, cystatin-C, and aldosterone serum concentrations by 28.76%, 28.41% and 29.43% (р&lt;0.05), respectively. Polymorphic site of <italic>CYP11B2</italic> (rs1799998) gene is associated with SBP and DBP increase (p&lt;0.05), reduced GFR preferably calculated by CKDEPI-cystatin C (F=10.79–14.45; p&lt;0.001) and elevated aldosterone content (F=55.84; p&lt;0.001), creatinine and cystatin-С as well (F=4.16–5.08; p&lt;0.05) mainly in the <italic>ТТ</italic>-genotype female carriers (p&lt;0.001). Hypertensive women with DM2 demonstrated stronger relations of <italic>CYP11B2</italic> gene polymorphic site with the increased aldosterone content (F=47.52; p&lt;0.001), than women without DM2 (p&lt;0.001) and male patients (p=0.014).<bold>Conclusions.</bold> Genetic variations involving <italic>CYP11B2</italic> might influence the kidney function, hypertension course, and severity via aldosterone secretion upregulation.</abstract>

Objective. The cytochrome 11B2 aldosterone synthase gene (CYP11B2) that links to aldosterone synthase enzyme synthesis changes and blood pressure regulation is of particular interest among the renin-angiotensin-aldosterone system encoding genes.Methods. One-hundred hypertensive patients with target-organ damaging (2nd stage), moderate, high or very high cardiovascular risk were involved in the case-control study. Mean age was 59.87±8.02 years. Diabetes Mellitus type 2 (DM2) was in 28 persons. Chronic kidney disease (CKD) was diagnosed in 29 persons according to the National Kidney Foundation recommendations (2012) after glomerular filtration rate (GFR) decline <60 ml/min/1.73m2 for ≥3 months (measured by CKD-EPI equations). Aldosterone, cystatin-C, and creatinine levels were measured in serum. Control group included 48 practically healthy persons of relevant age. Gene’s nucleotide polymorphism CYP11B2 (-344C/T) was examined by polymerase chain reaction.Results. CKD evolution in hypertensive patients followed by higher systolic and diastolic blood pressure (SBP, DBP) values increased creatinine, cystatin-C, and aldosterone serum concentrations by 28.76%, 28.41% and 29.43% (р<0.05), respectively. Polymorphic site of CYP11B2 (rs1799998) gene is associated with SBP and DBP increase (p<0.05), reduced GFR preferably calculated by CKDEPI-cystatin C (F=10.79–14.45; p<0.001) and elevated aldosterone content (F=55.84; p<0.001), creatinine and cystatin-С as well (F=4.16–5.08; p<0.05) mainly in the ТТ-genotype female carriers (p<0.001). Hypertensive women with DM2 demonstrated stronger relations of CYP11B2 gene polymorphic site with the increased aldosterone content (F=47.52; p<0.001), than women without DM2 (p<0.001) and male patients (p=0.014).Conclusions. Genetic variations involving CYP11B2 might influence the kidney function, hypertension course, and severity via aldosterone secretion upregulation.

The cytochrome 11B2 aldosterone synthase gene rs1799998 single nucleotide polymorphism determines elevated aldosterone, higher blood pressure, and reduced glomerular filtration, especially in diabetic female patients

Endocrine Regulations

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Objective. The cytochrome 11B2 aldosterone synthase gene (CYP11B2) that links to aldosterone synthase enzyme synthesis changes and blood pressure regulation is...

The cytochrome 11B2 aldosterone synthase gene rs1799998 single nucleotide polymorphism determines elevated aldosterone, higher blood pressure, and reduced glomerular filtration, especially in diabetic female patients

Publicado en línea: 27 ago 2020

Páginas: 217 - 226

DOI: https://doi.org/10.2478/enr-2020-0024

Palabras clave
CYP11B2 (rs1799998) gene, aldosterone, chronic kidney disease, hypertension, diabetes

© 2020 Larysa Sydorchuk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The cytochrome 11B2 aldosterone synthase gene rs1799998 single nucleotide polymorphism determines elevated aldosterone, higher blood pressure, and reduced glomerular filtration, especially in diabetic female patients

Publicado en línea: 27 ago 2020

Páginas: 217 - 226

DOI: https://doi.org/10.2478/enr-2020-0024

Palabras claveCYP11B2 (rs1799998) gene, aldosterone, chronic kidney disease, hypertension, diabetes

© 2020 Larysa Sydorchuk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
CYP11B2 (rs1799998) gene, aldosterone, chronic kidney disease, hypertension, diabetes