<abstract xmlns="http://www.w3.org/1999/xhtml">

Pinus sibirica (P. sibirica) is used in folk medicine for centuries in treatment of various diseases due to its anti-inflammatory, antioxidant, and antimicrobial activity. The present study was focused on formulating semi-solid topical cream and gel preparations with P. sibirica essential oil (EOPS) and on evaluation of their stability during three months of storage. After preparation of semi-solid formulations (cream and gel), we followed parameters such as organoleptic characteristics, pH values, electrical conductivity, phase separation, washability, swelling index and microbiological contamination. Tests for physical and microbiological stability were performed considering different storage conditions at temperature (4±2° C, 25±2°C and 40±2°C) for 12 weeks. All test were performed at sampling time points of 7th, 30th, 60th, 90th day after storage. Cream with EOPS was white colour, it is homogenous with pleasant odor characteristic to used EOPS while gel with EOPS was transparent, it is homogenous with specific odor. After application to skin, EOPS cream was leaving non-greasy film while gel with EOPS was leaving sticky film and cooling effect. In all tested samples, we observed no phase separation and pH values were optimal for skin application. During three months of storage at different conditions, all tested formulations were stable and addition of EOPS did not cause any changes in followed parameters. Both cream and gel formulation remained stable since we did not notice any significant changes in all followed parameters during storage at different temperatures. Addition of 0.5% of EOPS did not change visual, sensory and microbial properties of formulations.
</abstract>

Pinus sibirica (P. sibirica) is used in folk medicine for centuries in treatment of various diseases due to its anti-inflammatory, antioxidant, and antimicrobial activity. The present study was focused on formulating semi-solid topical cream and gel preparations with P. sibirica essential oil (EOPS) and on evaluation of their stability during three months of storage. After preparation of semi-solid formulations (cream and gel), we followed parameters such as organoleptic characteristics, pH values, electrical conductivity, phase separation, washability, swelling index and microbiological contamination. Tests for physical and microbiological stability were performed considering different storage conditions at temperature (4±2° C, 25±2°C and 40±2°C) for 12 weeks. All test were performed at sampling time points of 7th, 30th, 60th, 90th day after storage. Cream with EOPS was white colour, it is homogenous with pleasant odor characteristic to used EOPS while gel with EOPS was transparent, it is homogenous with specific odor. After application to skin, EOPS cream was leaving non-greasy film while gel with EOPS was leaving sticky film and cooling effect. In all tested samples, we observed no phase separation and pH values were optimal for skin application. During three months of storage at different conditions, all tested formulations were stable and addition of EOPS did not cause any changes in followed parameters. Both cream and gel formulation remained stable since we did not notice any significant changes in all followed parameters during storage at different temperatures. Addition of 0.5% of EOPS did not change visual, sensory and microbial properties of formulations.

Developement and Stability Evaluation of Natural Topical Formulations Containing <italic>Pinus sibirica</italic> Essential Oil

Department of Pharmacy, Faculty of Medical Sciences

Department of Physiology, Faculty of Medical Sciences

Sechenov First Moscow State Medical University

Experimental and Applied Biomedical Research (EABR)

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Developement and Stability Evaluation of Natural Topical Formulations Containing  Essential Oil

Pinus sibirica (P. sibirica) is used in folk medicine for centuries in treatment of various diseases due to its anti-inflammatory, antioxidant, and...

Developement and Stability Evaluation of Natural Topical Formulations Containing Pinus sibirica Essential Oil

Publicado en línea: 06 abr 2024

Páginas: -

Recibido: 26 ene 2024

Aceptado: 07 mar 2024

DOI: https://doi.org/10.2478/eabr-2024-0003

Palabras clave
Pinus sibirica, stability, topical formulations, essential oil

© 2024 Branislav Petrovic et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Developement and Stability Evaluation of Natural Topical Formulations Containing Pinus sibirica Essential Oil

Publicado en línea: 06 abr 2024

Páginas: -

Recibido: 26 ene 2024

Aceptado: 07 mar 2024

DOI: https://doi.org/10.2478/eabr-2024-0003

Palabras clavePinus sibirica, stability, topical formulations, essential oil

© 2024 Branislav Petrovic et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
Pinus sibirica, stability, topical formulations, essential oil