Fish diversity of a riverine ecosystem became reduced due to different manmade and natural calamities. This study investigated the present fish biodiversity status of the Dharla River located (25.8103° N, 89.6487° E) in the northern part of Bangladesh from January to December 2018. In this study, the existing fish biodiversity status of the Dharla River was estimated in terms of diversity indices and threatened status (both global and local). Data was collected from the three sampling sites of the river using different fishing gears and three selected fish markets located in the Kurigram district of Bangladesh. A total of 76 fish species were identified belonging to 8 orders, 26 families and 57 genera. The Cyprinidae was the most dominant family represented by 14 species followed by the Danionidae (13 species), Bagridae (8 species) and few minor families. Apart from the indigenous species, nine exotic fish species were also recorded. The Shannon-Weaver diversity (