<abstract xmlns="http://www.w3.org/1999/xhtml">

World-wide, Indonesia is ranked 17th in oral cancers, with deaths reaching 2.326 cases. Of the oral cancers, 90% are squamous cell carcinoma (HSC-3). Unfortunately, conventional cancer therapy still has many ill side effects. Therefore, pharmacologists have looked for natural ingredients to prevent the growth of oral cancer cells. One source is <italic>Cymbopogon citratus</italic> leaf. Research shows that the active compound of <italic>C. citratus</italic> leaf is a chemopreventive, doing so by increasing the production of re-active oxygen species (ROS) to induce apoptosis in cancer cells. The active compound of <italic>C. citratus</italic> leaf has low stability and solubility, so it is necessary to use an encapsulation matrix such as chitosan, and modify it into smaller particles to increase its effectiveness.
Purpose is determining the effect of nano chitosan encapsulation of <italic>C. citratus</italic> leaf ethanol extract (NCECC) on the reactive oxygen species of HSC-3 tongue cancer cells. This study is divided into ten groups – without treatment, doxorubicin (positive control), hydrogen peroxide, nano chitosan and C. citratus leaf extract groups, and five groups of NCECC treatment – concentrations of 100%, 75%, 50%, 25%, and 12.5%, respectively. The 100% NCECC group showed the highest ROS concentration (p&lt;0.05), compared to 75%, 50%, 25%, 12.5% NCECC groups, and the 100% NCECC was higher than the positive control group. NCECC is effective in inducing oxidative stress on HSC-3 through increased production of ROS. Moreover, the higher the encapsulation concentration given, the greater the increase in ROS production.
</abstract>

World-wide, Indonesia is ranked 17th in oral cancers, with deaths reaching 2.326 cases. Of the oral cancers, 90% are squamous cell carcinoma (HSC-3). Unfortunately, conventional cancer therapy still has many ill side effects. Therefore, pharmacologists have looked for natural ingredients to prevent the growth of oral cancer cells. One source is Cymbopogon citratus leaf. Research shows that the active compound of C. citratus leaf is a chemopreventive, doing so by increasing the production of re-active oxygen species (ROS) to induce apoptosis in cancer cells. The active compound of C. citratus leaf has low stability and solubility, so it is necessary to use an encapsulation matrix such as chitosan, and modify it into smaller particles to increase its effectiveness.
Purpose is determining the effect of nano chitosan encapsulation of C. citratus leaf ethanol extract (NCECC) on the reactive oxygen species of HSC-3 tongue cancer cells. This study is divided into ten groups – without treatment, doxorubicin (positive control), hydrogen peroxide, nano chitosan and C. citratus leaf extract groups, and five groups of NCECC treatment – concentrations of 100%, 75%, 50%, 25%, and 12.5%, respectively. The 100% NCECC group showed the highest ROS concentration (p<0.05), compared to 75%, 50%, 25%, 12.5% NCECC groups, and the 100% NCECC was higher than the positive control group. NCECC is effective in inducing oxidative stress on HSC-3 through increased production of ROS. Moreover, the higher the encapsulation concentration given, the greater the increase in ROS production.

Nano chitosan encapsulation of <italic>Cymbopogon citratus</italic> leaf extract promotes ROS induction leading to apoptosis in human squamous cells (HSC-3)

Dentristry Student of Faculty of Denstistry

Department of Histology, Faculty of Denstistry

Department of Oral Biology, Faculty of Denstistry

Department of Physiology, Faculty of Denstistry

Current Issues in Pharmacy and Medical Sciences

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Nano chitosan encapsulation of  leaf extract promotes ROS induction leading to apoptosis in human squamous cells (HSC-3)

World-wide, Indonesia is ranked 17th in oral cancers, with deaths reaching 2.326 cases. Of the oral cancers, 90% are squamous cell carcinoma (HSC-3)....

Nano chitosan encapsulation of Cymbopogon citratus leaf extract promotes ROS induction leading to apoptosis in human squamous cells (HSC-3)

Publicado en línea: 08 oct 2021

Páginas: 134 - 137

Recibido: 03 mar 2021

Aceptado: 20 jul 2021

DOI: https://doi.org/10.2478/cipms-2021-0026

Palabras clave
reactive oxygen species, encapsulation, nano chitosan, leaf extract, HSC-3

© 2021 Sasqia Faadillah Andikoputri et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Nano chitosan encapsulation of Cymbopogon citratus leaf extract promotes ROS induction leading to apoptosis in human squamous cells (HSC-3)

Publicado en línea: 08 oct 2021

Páginas: 134 - 137

Recibido: 03 mar 2021

Aceptado: 20 jul 2021

DOI: https://doi.org/10.2478/cipms-2021-0026

Palabras clavereactive oxygen species, encapsulation, nano chitosan, leaf extract, HSC-3

© 2021 Sasqia Faadillah Andikoputri et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Palabras clave
reactive oxygen species, encapsulation, nano chitosan, leaf extract, HSC-3