<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The study included 40 strains of <italic>Malassezia pachydermatis </italic>obtained in pure culture from external ear canal of dogs and the reference strain. Protein fractions were received by mechanical disruption of the fungal cells. Denaturing protein electrophoresis was performed according to Laemmli method. More than 90% of the all tested strains were characterised by the presence of the following protein fractions: 83.0; 77.0; 59.0; 55.0; 48.0; 38.0; 35.0; 28.0, and 27.0 kDa. In other regions of electrophoregrams, a relatively high differentiation was observed. The strains isolated from animals with otitis externa were characterised by the presence of the following protein fractions: 75.0; 61.0; 52.0; 36.0; 19.5; 16.0; 13.8, and 13.0 kDa. These fractions were absent in the commensal strains. The use of statistical analysis confirmed the obtained results and allowed to generate dendrogram grouping potentially pathogenic and commensal strains on two different branches. Such results may indicate significant differences between protein profiles of strains of <italic>M. pachydermatis </italic>isolated from healthy and diseased animals.</p></abstract>

The study included 40 strains of Malassezia pachydermatis obtained in pure culture from external ear canal of dogs and the reference strain. Protein fractions were received by mechanical disruption of the fungal cells. Denaturing protein electrophoresis was performed according to Laemmli method. More than 90% of the all tested strains were characterised by the presence of the following protein fractions: 83.0; 77.0; 59.0; 55.0; 48.0; 38.0; 35.0; 28.0, and 27.0 kDa. In other regions of electrophoregrams, a relatively high differentiation was observed. The strains isolated from animals with otitis externa were characterised by the presence of the following protein fractions: 75.0; 61.0; 52.0; 36.0; 19.5; 16.0; 13.8, and 13.0 kDa. These fractions were absent in the commensal strains. The use of statistical analysis confirmed the obtained results and allowed to generate dendrogram grouping potentially pathogenic and commensal strains on two different branches. Such results may indicate significant differences between protein profiles of strains of M. pachydermatis isolated from healthy and diseased animals.

Comparative Analysis of Protein Profiles of Malassezia Pachydermatis Strains Isolated from Healthy Dogs and Dogs with Otitis Externa

Institute of Biological Bases of Animal Diseases, Sub-Department of Veterinary Microbiology, Faculty of Veterinary Medicine

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The study included 40 strains of Malassezia pachydermatis obtained in pure culture from external ear canal of dogs and the reference strain. Protein fractions...

Comparative Analysis of Protein Profiles of Malassezia Pachydermatis Strains Isolated from Healthy Dogs and Dogs with Otitis Externa

Publicado en línea: 15 mar 2013

Páginas: 41 - 46

DOI: https://doi.org/10.2478/bvip-2013-0008

© 2013

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.