Single nucleotide polymorphisms in IL-1A RS1800587, IL-1B RS1143634 and vitamin D receptor rs731236 in stage III grade B/C periodontitis

Genotypic and allelic frequency of IL-1A, IL-1B and VDR polymorphisms in Grade B and C periodontitis and healthy population

Gene SNPs (rs number)	Genotypes and Alleles		Grade B Periodontitis N=51	Grade C Periodontitis N=49	Healthy N= 100 N (%)	Grade B Periodontitis VS Healthy			Grade C Periodontitis VS Healthy			Grade B Periodontitis VS Grade C Periodontitis
			N (%)	N (%)		χ2 (p value)	OR (95% CI)	Fisher exact (p value)	χ2 (p value)	OR (95% CI)	Fisher exact (p value)	χ2 (p value)	OR (95% CI)	Fisher exact (p value)
ILIA889 (rsl800587)	CC		26(51)	23 (46.9)	56 (56)	0.608	0.718(0.179-2.874) 0.539(0.129-2.175)	0.749	0.403	1.623 (0.518-5.082) 1.167(0.362-3.756)	0.543	0.277	2.261 (0.507-10.07) 2.200(0.485-9.983)	0.470
	CT		22(43.1)	20(40.8)	35 (35)	0.323		0.515	0.796		1.000	0.300		0.485
	TT		3(5.9)	6(12.2)	9(9)
	C allele		74(72.5)	66(67.3)	147(73.5)	0.806	1.07(0.625-1.831)	0.890	0.241	1.372(0.809-2.323)	0.273	0.422	1.281 (0.699-2.349)	0.444
	T allele		28 (27.5)	32(32,7)	53 (26.5)
IL1B+3954 (rsll43634)	CC		29(56.9)	31(63.3)	74(74)	0.304	0.490(0.123-1.953) 1.071 (0.249-4.603)	0.445	0.635	0.698(0.157-3.103) 1.190(0.246-5.794)	0.695	0.659	0.702(0.144-3.407) 0.900(0.173-4.669)	0.709
	CT		18(35.3)	15(30.6)	21(21)	0.926		1.000	0.828		1.000	0.900		1.000
	TT		4(7.8)	3(6.1)	5(5)
	C allele		76(74.5)	77(78.6)	169(84.5)	0.036	1.865 (1.037-3.355)	0.043	0.205	0.673(0.363-1.245)	0.255	0.498	0.797(0.413-1.537)	0.510
	T allele		26 (25.5)	21(21.4)	31(15.5)
VDR TaqI (rs731236)	CC		9(17.6)	10(20.4)	10(10)	0.024	3.392(1.142- 10.075)	0.038	0.136	3.769(1.295-10.975) 0.831 (0.388-1.780)	0.188	0.564	1.592(0,515-4,922) 3.207(1.286-7.997)	0.300
	CT		29(56.9)	16(32.7)	41(41)	0.012	2.666(1.229-5.785)	0.015	0.634		0.702	0.011		0.014
	TT		13(25.5)	23 (46.9)	49(49)
	C allele		47(46.1)	36(36.7)	61(30.5)	0.008	1.947(1.190-3.185)	0.011	0.281	1.323 (0.795-2.202)	0.295	0.180	1.472(0.836-2.592)	0.198
	T allele		55 (53.9)	62(63.3)	139 (69.5)

Genotypic frequency of IL-1A, IL-1B and VDR polymorphisms in periodontitis and healthy population

Gene SNPs (rs number)	Genotype	Periodontitis N= 100 N (%)	Healthy N=100 N (%)	χ2 P value	OR (95% CI)	Fisher exact P value
IL-1A-889 (rs1800587)	CC	49 (49)	56 (56)	0.793	1.142(0.420-3.107)	0.804
	CT	42 (42)	35 (35)	0.727	0.833(2.327-2.327)	0.796
	TT	9 (9)	9 (9)
	HWE	1.000	0.309
IL-1B+3954 (rs1143634)	CC	60 (60)	74 (74)	0.371	1.726 (0.52-5.716)	1.000
	CT	33 (33)	21 (21)	0.858	0.890 0.249-3.176)	0.333
	TT	7 (7)	5 (5)
	HWE	0.867	0.005
VDR TaqI (rs731236)	CC	19 (19)	10 (10)	0.031	2.586(1,074-6.224)	0.034
	CT	45 (45)	41 (41)	0.192	1.494 (0.817-2.732)	0.222
	TT	36 (36)	49 (49)
	HWE	0.464	0.742

Periodontal clinical parameters in study population

Parameters	Periodontitis N=100 Mean±SD	Healthy N=100 Mean±SD	P value
Age Range (years)	39.61±8.74 (22-59)	29.50±4.98 (21-55)	0.000*
Gender (%) Male Female	43 (43) 57 (57)	38 (38) 62 (62)	0.565†
PD (mm)	2.88±0.79	1.79±0.33	0.000*
CAL (mm)	3.14±0.88	1.81±1.79	0.000*
PI	1.70±0.45	0.34±0.20	0.000*
GI	1.56±0.41	0.26±0.23	0.000*
BOP (%)	58.82±21.91	8.77±6.25	0.000*

Model wise genotypic distribution of IL-1A, IL-1B and VDR in periodontitis and healthy population

Genotypes and alleles	Periodontitis N (%)	Healthy N (%)	OR (95% CI)	χ2 P value
IL1A-889 (rs1800587)
C	140 (70)	147 (73.5)	1.188 (0.768-1.838)	0.437
T ref	60 (30)	53 (26.5)
Codominant model
CC	49 (49)	56 (56)	1.142 (0.420-3.107)	0.793
CT	42 (42)	35 (35)	0.833 (2.327-2.327)	0.727
TT ref	9 (9)	9 (9)
Dominant model
CC ref	49	56
CT+TT	51	44	0.754 (0.432-1.316)	0.322
Recessive model
CC+CT ref	91	91
TT	9	9	1.000 (0.380-2.634)	1.000
Overdominant model
CC+TT ref	58	65
CT	42	35	0.744 (0.420-1.317)	0.309
IL1B+3954 (rs1143634)
C	153 (76.5)	169 (84.5)	1.167 (1.012-2.770)	0.045
T ref	47 (23.5)	31 (15.5)
Codominant model
CC	60 (60)	74 (74)	1.726 (0.52-5.716)	0.371
CT	33 (33)	21 (21)	0.890 (0.249-3.176)	0.858
TT ref	7 (7)	5 (5)
Dominant model
CC ref	60 (60)	74 (74)
CT+TT	40 (40)	26 (26)	0.527 (0.289-0.960)	0.035
Recessive model
CC+CT ref	93 (93)	95 (95)
TT	7 (7)	5 (5)	0.699 (0.214-2.282)	0.522
Overdominant model
CC+TT ref	67 (67)	79 (79)	0.5397 (0,2855-1.021)	0.058
CT	33 (33)	21 (21)
VDR TaqI (rs731236)
T	117 (58.5)	139 (69.5)	1.616 (1.07-2.44)	0.022
C ref	83 (41.5)	61 (30.5)
Codominant model
TT	36 (36)	49 (49)	2.586 (1.074-6.224)	0.034
CT	45 (45)	41 (41)	1.73 (0.721-4.152)	0.218
CC ref	19 (19)	10 (10)
Dominant model
TT ref	36 (36)	49 (49)
CT+CC	64 (64)	51 (51)	0.585 (0.332-1.031)	0.063
Recessive model
TT+CT ref	81 (81)	90 (90)
CC	19 (19)	10 (10)	0.474 (0.208-1.078)	0.075
Overdominant model
TT+CC ref	55 (55)	59 (59)
CT	45 (45)	41 (41)	0.845 (0.485-1.487)	0.568

Allelic frequency of IL-1A, IL-1B and VDR polymorphisms in periodontitis and healthy population

Gene SNPs (rs number)	Allele	Periodontitis N= 100 N (%)	Healthy N=100 N (%)	χ2 P value	OR (95% CI)	Fisher exact P value
IL1A-889	C	140 (70)	147 (73.5)	0.437	1.188 (0.768-1.838)	0.505
(rs1800587)	T	60 (30)	53 (26.5)
IL1B+3954	C	153 (76.5)	169 (84.5)	0.045	1.167 (1.012-2.770)	0.058
(rs1143634)	T	47 (23.5)	31 (15.5)
VDR TaqI	C	83 (41.5)	61 (30.5)	0.022	1.61 (1.071-2.441)	0.029
(rs731236)	T	117 (58.5)	139 (69.5)