<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Importunate high-risk HPV (HR-HPV) infection is the most common trigger for the cervical carcinogenesis process. In this respect, the presence of cancer can be imputed to telomere lengthening or shortening. This paper explores the possible correlation between relative telomere length and viral load in two groups of women, namely: those with high-risk HPV infection and those who do not have this infection. Thus, samples comprising of 50 women in each group were evaluated for this research. The Amplisens HPV HCR screen-titre-FRT PCR kite was employed for quantitative analysis. Relative telomere length was quantified by real-time PCR. In each of the two HPV load groups, there was no correlation between age and telomere length. Telomere shortening was found in the cervical cell samples of women with high HPV loads, compared with women in the control group. Telomere shortening is associated with elevated HPV loads.</p>
</abstract>

Importunate high-risk HPV (HR-HPV) infection is the most common trigger for the cervical carcinogenesis process. In this respect, the presence of cancer can be imputed to telomere lengthening or shortening. This paper explores the possible correlation between relative telomere length and viral load in two groups of women, namely: those with high-risk HPV infection and those who do not have this infection. Thus, samples comprising of 50 women in each group were evaluated for this research. The Amplisens HPV HCR screen-titre-FRT PCR kite was employed for quantitative analysis. Relative telomere length was quantified by real-time PCR. In each of the two HPV load groups, there was no correlation between age and telomere length. Telomere shortening was found in the cervical cell samples of women with high HPV loads, compared with women in the control group. Telomere shortening is associated with elevated HPV loads.

Association of Relative Telomere Length and Risk of High Human Papillomavirus Load in Cervical Epithelial Cells

Department of Medical Laboratory Techniques

Al-Dour Technical Institute, Northern Technical University

Genetics Department of Academy of Biology and Biotechnology

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Importunate high-risk HPV (HR-HPV) infection is the most common trigger for the cervical carcinogenesis process. In this respect, the presence of cancer can be...

HPV group		Control group		2^−ΔΔCt	p-value
Number	Mean of T/S ± SDE	Number	Mean of T/S ± SDE	2^−ΔΔCt	p-value
50	1144.06 ± 64.085	50	1571.21 ± 111.55	2.5925 ×10⁻¹²⁹	0.0014
HPV load 4-5lg				2.5925 ×10⁻¹²⁹	0.0014
21	1035.12 ± 65.926			4.1809 × 10⁻¹⁶²	0.0001
HPV load >5lg				4.1809 × 10⁻¹⁶²	0.0001
29	1222.93 ± 98.052			1.4454 × 10⁻¹⁰⁵	0.0217

Primer/oligomer	Sequence
Primer Telo F	5′- cgg-ttt-gtt-tgg-gtt-tgg-gtt-tgg-gtt-tgg-gtt-tgg-gtt-3′
Primer Telo R	5′- ggc-ttg-cct-tac-cct-tac-cct-tac-cct-tac-cct-tac-cct-3′
Primer 36B4 F	5′- cag-caa-gtg-gga-agg-tgt-aat-cc-3′
Primer 36B4 R	5′-ccc-att-cta-tca-tca-acg-ggt-aca-a-3′

Association of Relative Telomere Length and Risk of High Human Papillomavirus Load in Cervical Epithelial Cells

Article Category: Original Article

Publicado en línea: 05 jun 2022

Páginas: 65 - 70

DOI: https://doi.org/10.2478/bjmg-2021-0026

Palabras clave
Telomere, Human papillomavirus, Viral load, Cervical cancer, Real-time PCR

© 2021 A H Albosale et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Figure 2

The relative telomere lengths among the studied groups.

The sequence of primers used in real-time PCR.

Association of Relative Telomere Length and Risk of High Human Papillomavirus Load in Cervical Epithelial Cells

Article Category: Original Article

Publicado en línea: 05 jun 2022

Páginas: 65 - 70

DOI: https://doi.org/10.2478/bjmg-2021-0026

Palabras claveTelomere, Human papillomavirus, Viral load, Cervical cancer, Real-time PCR

© 2021 A H Albosale et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Figure 2

The relative telomere lengths among the studied groups.

The sequence of primers used in real-time PCR.

Palabras clave
Telomere, Human papillomavirus, Viral load, Cervical cancer, Real-time PCR