<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Obesity is as a global health problem due to its interaction with complex chronic disorders such as cardiovascular disorders, type 2 diabetes mellitus (T2DM) and cancer. Despite the fact that pathogenesis of obesity is not yet clearly understood, it is associated with a combination of psychological, environmental and various genetic factors. Here, employing a case-control design, we aimed to examine the effects of the <italic>GHRL</italic> c.152C>T (p.Arg51Gln) (rs34911341) and c.214G>T (p.Leu72Met) (rs696217) markers on susceptibility to obesity in a Turkish-Cypriot population, as well as to evaluate whether these markers affect biochemical parameters and show their putative functional consequences. This study involved 211 Turkish-Cypriot subjects (106 obese and 95 non obese). Genotyping for the <italic>GHRL</italic> gene polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Our results indicate that the <italic>GHRL</italic> Leu72Met polymorphism was found to be significantly higher in obese patients, with respect to genotypic (<italic>p</italic> = 0.0012) and allelic (<italic>p</italic> = 0.0005) frequencies. Strikingly, the rs696217 GT genotype (heterozygous) had significantly lower serum high-density lipoprotein cholesterol (HDL-C) (<italic>p</italic> = 0.015) than GG (wild type) genotypes. Overall, Leu72Met susceptibility variant may be considered as risk and crucial marker for both obesity and cholesterol metabolism in the community of Turkish-Cypriots. Thus, the dual effect of the <italic>GHRL</italic> gene Leu72Met variant may be used for clinical diagnosis.</p></abstract>

Obesity is as a global health problem due to its interaction with complex chronic disorders such as cardiovascular disorders, type 2 diabetes mellitus (T2DM) and cancer. Despite the fact that pathogenesis of obesity is not yet clearly understood, it is associated with a combination of psychological, environmental and various genetic factors. Here, employing a case-control design, we aimed to examine the effects of the GHRL c.152C>T (p.Arg51Gln) (rs34911341) and c.214G>T (p.Leu72Met) (rs696217) markers on susceptibility to obesity in a Turkish-Cypriot population, as well as to evaluate whether these markers affect biochemical parameters and show their putative functional consequences. This study involved 211 Turkish-Cypriot subjects (106 obese and 95 non obese). Genotyping for the GHRL gene polymorphisms was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Our results indicate that the GHRL Leu72Met polymorphism was found to be significantly higher in obese patients, with respect to genotypic (p = 0.0012) and allelic (p = 0.0005) frequencies. Strikingly, the rs696217 GT genotype (heterozygous) had significantly lower serum high-density lipoprotein cholesterol (HDL-C) (p = 0.015) than GG (wild type) genotypes. Overall, Leu72Met susceptibility variant may be considered as risk and crucial marker for both obesity and cholesterol metabolism in the community of Turkish-Cypriots. Thus, the dual effect of the GHRL gene Leu72Met variant may be used for clinical diagnosis.

Dual effect of the <italic>GHRL</italic> gene variant in the molecular pathogenesis of obesity

Balkan Journal of Medical Genetics

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Dual effect of the  gene variant in the molecular pathogenesis of obesity

Obesity is as a global health problem due to its interaction with complex chronic disorders such as cardiovascular disorders, type 2 diabetes mellitus (T2DM)...

Dual effect of the GHRL gene variant in the molecular pathogenesis of obesity

Article Category: Original Article

Publicado en línea: 27 jul 2021

Páginas: 27 - 34

DOI: https://doi.org/10.2478/bjmg-2021-0011

Palabras clave
Arg51Gln, Ghrelin, gene, Leu72 Met, Obesity, Turkish-Cypriot population

© 2021 Becer E, Ergoren MC, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Dual effect of the GHRL gene variant in the molecular pathogenesis of obesity

Article Category: Original Article

Publicado en línea: 27 jul 2021

Páginas: 27 - 34

DOI: https://doi.org/10.2478/bjmg-2021-0011

Palabras claveArg51Gln, Ghrelin, gene, Leu72 Met, Obesity, Turkish-Cypriot population

© 2021 Becer E, Ergoren MC, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
Arg51Gln, Ghrelin, gene, Leu72 Met, Obesity, Turkish-Cypriot population