<abstract xmlns="http://www.w3.org/1999/xhtml">

<sec><h3>Aim of investigation</h3>
<p>Comparison of an electrochemiluminescence technique (ECL, applied in Cobas 6000 instrument), an enzyme-linked immunosorbent assay (ELISA, applied in CHORUS instrument) and an enzyme-linked fluorescent assay (ELFA, applied in MINI-VIDAS instrument) for early diagnosis of <italic>Toxoplasma gondii</italic> infection in pregnant women. Medical diagnosis is required to determine the most sensitive techniques to diagnose this pathogen, in the framework of which is developed this scientific work. This is very important due to the multiple fetal infections during pregnancy.</p>
</sec>
<sec><h3>Methods</h3>
<p>ECL, ELISA and ELFA techniques are used to detect anti-toxoplasma IgM antibodies in pregnant women, during the first trimester of pregnancy. 200 samples were analyzed with each technique and sensitivity and specificity are evaluated for each of them.</p>
</sec>
<sec><h3>Results</h3>
<p>ECL technique has resulted in higher sensitivity (100%) and specificity (99.3%), while ELISA technique has resulted in lower sensitivity (87.5%) and specificity (97.3%).</p>
</sec>
<sec><h3>Conclusion</h3>
<p>Analysis of the results confirmed the usefulness of ECL technique for an early diagnosis of <italic>Toxoplasma gondii</italic> infections in pregnant women. Anyway, for diagnostic purposes, the results should always be assessed in conjuction with the patient’s medical history and other clinical examinations.</p>
</sec>
</abstract>

Aim of investigation
Comparison of an electrochemiluminescence technique (ECL, applied in Cobas 6000 instrument), an enzyme-linked immunosorbent assay (ELISA, applied in CHORUS instrument) and an enzyme-linked fluorescent assay (ELFA, applied in MINI-VIDAS instrument) for early diagnosis of Toxoplasma gondii infection in pregnant women. Medical diagnosis is required to determine the most sensitive techniques to diagnose this pathogen, in the framework of which is developed this scientific work. This is very important due to the multiple fetal infections during pregnancy.

Methods
ECL, ELISA and ELFA techniques are used to detect anti-toxoplasma IgM antibodies in pregnant women, during the first trimester of pregnancy. 200 samples were analyzed with each technique and sensitivity and specificity are evaluated for each of them.

Results
ECL technique has resulted in higher sensitivity (100%) and specificity (99.3%), while ELISA technique has resulted in lower sensitivity (87.5%) and specificity (97.3%).

Conclusion
Analysis of the results confirmed the usefulness of ECL technique for an early diagnosis of Toxoplasma gondii infections in pregnant women. Anyway, for diagnostic purposes, the results should always be assessed in conjuction with the patient’s medical history and other clinical examinations.

Comparison of three immunoassays for detection of anti-toxoplasma IgM antibodies in pregnant women

Department of Biology, Faculty of Technical Sciences

Balkan Journal of Interdisciplinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License.

Aim of investigation
Comparison of an electrochemiluminescence technique (ECL, applied in Cobas 6000 instrument), an enzyme-linked immunosorbent assay (ELISA,...

Comparison of three immunoassays for detection of anti-toxoplasma IgM antibodies in pregnant women

Publicado en línea: 14 dic 2023

Páginas: 13 - 22

DOI: https://doi.org/10.2478/bjir-2023-0014

Palabras clave
Electrochemiluminescence, ELISA, ELFA, Toxoplasma gondii, sensitivity

© 2023 Blerta Laze, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License.

Comparison of three immunoassays for detection of anti-toxoplasma IgM antibodies in pregnant women

Publicado en línea: 14 dic 2023

Páginas: 13 - 22

DOI: https://doi.org/10.2478/bjir-2023-0014

Palabras claveElectrochemiluminescence, ELISA, ELFA, Toxoplasma gondii, sensitivity

© 2023 Blerta Laze, published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License.

Palabras clave
Electrochemiluminescence, ELISA, ELFA, Toxoplasma gondii, sensitivity