<abstract xmlns="http://www.w3.org/1999/xhtml">The aim of the present study was to determine and compare _-tocopherol and ergocalciferol content in four macroalgae from Bulgarian Black sea coast. Ulva rigida, Cladophora vagabunda, Cystoseira barbata and Cystoseira crinita were used for evaluation of corresponding fat soluble vitamins content. The sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction. Ergocalciferol (vitamin D2) and α-tocopherol (vitamin E) were analyzed simultaneously using HPLC/UV/FL system (Thermo Scientific Spectra SYSTEM) equipped with RP analytical column. The mobile phase was composed of 97:3 = MeOH:H2O. Ergocalciferol was monitored by UV detection at λmax = 265nm, while α-tocopherol was detected by fluorescence at λex=288nm and λem=332nm. Alpha-tocopherol content in algal tissues ranged from 1.68±0.38mg/100g d.w. in Cladophora vagabunda to 29.13±1.08mg/100g d.w. in Cystoseira barbata. Ergocalciferol was detected only in Ulva rigida samples. </abstract>

The aim of the present study was to determine and compare _-tocopherol and ergocalciferol content in four macroalgae from Bulgarian Black sea coast. Ulva rigida, Cladophora vagabunda, Cystoseira barbata and Cystoseira crinita were used for evaluation of corresponding fat soluble vitamins content. The sample preparation procedure includes alkaline saponification, followed by liquid-liquid extraction. Ergocalciferol (vitamin D2) and α-tocopherol (vitamin E) were analyzed simultaneously using HPLC/UV/FL system (Thermo Scientific Spectra SYSTEM) equipped with RP analytical column. The mobile phase was composed of 97:3 = MeOH:H2O. Ergocalciferol was monitored by UV detection at λmax = 265nm, while α-tocopherol was detected by fluorescence at λex=288nm and λem=332nm. Alpha-tocopherol content in algal tissues ranged from 1.68±0.38mg/100g d.w. in Cladophora vagabunda to 29.13±1.08mg/100g d.w. in Cystoseira barbata. Ergocalciferol was detected only in Ulva rigida samples.

Alpha-tocopherol and ergocalciferol contents of some macroalgae from Bulgarian Black Sea coast

Department of Chemistry, Medical University of Varna, 55 Marin Drinov str., Varna, Bulgaria

Ovidius University Annals of Chemistry

The aim of the present study was to determine and compare _-tocopherol and ergocalciferol content in four macroalgae from Bulgarian Black sea coast. Ulva...

Alpha-tocopherol and ergocalciferol contents of some macroalgae from Bulgarian Black Sea coast

Publicado en línea: 20 oct 2013

Páginas: 13 - 16

DOI: https://doi.org/10.2478/auoc-2013-0002

Palabras clave
macroalgae, ergocalciferol, α-tocopherol, HPLC

This content is open access.

Alpha-tocopherol and ergocalciferol contents of some macroalgae from Bulgarian Black Sea coast

Publicado en línea: 20 oct 2013

Páginas: 13 - 16

DOI: https://doi.org/10.2478/auoc-2013-0002

Palabras clavemacroalgae, ergocalciferol, α-tocopherol, HPLC

This content is open access.

Palabras clave
macroalgae, ergocalciferol, α-tocopherol, HPLC