<abstract xmlns="http://www.w3.org/1999/xhtml">

This study aimed to evaluate the effects of dietary probiotic supplementation of <italic>Lactobacillus helveticus</italic> on growth, digestive enzymes, and hematological, biochemical, immune, and antioxidant parameters, as well as intestinal microbiota of rainbow trout (<italic>Oncorhynchus mykiss</italic>). Fish (35.46±0.9 g) were fed with different levels of dietary <italic>L. helveticus</italic>: control, 1 × 106, 1 × 107, 1 × 108, and 1 × 109 at high stocking density (80 kg m−3) for 60 days. Results indicated that growth performance significantly improved in probiotic supplemented fish (P&lt;0.05). Digestive enzyme parameters revealed that supplementation could significantly increase amylase, protease, and lipase (P&lt;0.05). The treated groups showed significant improvements in serum immune parameters including lysozyme (LYZ), alternative complement (ACH50), respiratory burst activity (RBA), and myeloperoxidase (MPO) (P&lt;0.05). Total protein (TP), albumin (ALB), and globulin (GLO) increased in fish fed experimental diets (P&lt;0.05). Lactate dehydrogenase (LDH) activity was significantly lower in fish fed dietary additives (P&lt;0.05) while white blood cells (WBC), lymphocytes, neutrophils, hematocrit (Hct), red blood cells (RBC) were significantly enhanced (P&lt;0.05). Fish fed with supplemented diets showed significantly enhanced antioxidant status, catalase (CAT) and superoxide dismutase (SOD). Malondialdehyde (MDA) content was significantly lower in fish fed dietary additives (P&lt;0.05). Lactic acid bacteria (LAB) in the treatment groups were significantly increased (P&lt;0.05). In conclusion, dietary supplementation of <italic>L. helveticus</italic> reduced detrimental effects of high stocking density on growth performance and immune response. It appears that <italic>L. helveticus</italic> can be recommended as a beneficial probiotic feed additive for rainbow trout.
</abstract>

This study aimed to evaluate the effects of dietary probiotic supplementation of Lactobacillus helveticus on growth, digestive enzymes, and hematological, biochemical, immune, and antioxidant parameters, as well as intestinal microbiota of rainbow trout (Oncorhynchus mykiss). Fish (35.46±0.9 g) were fed with different levels of dietary L. helveticus: control, 1 × 106, 1 × 107, 1 × 108, and 1 × 109 at high stocking density (80 kg m−3) for 60 days. Results indicated that growth performance significantly improved in probiotic supplemented fish (P<0.05). Digestive enzyme parameters revealed that supplementation could significantly increase amylase, protease, and lipase (P<0.05). The treated groups showed significant improvements in serum immune parameters including lysozyme (LYZ), alternative complement (ACH50), respiratory burst activity (RBA), and myeloperoxidase (MPO) (P<0.05). Total protein (TP), albumin (ALB), and globulin (GLO) increased in fish fed experimental diets (P<0.05). Lactate dehydrogenase (LDH) activity was significantly lower in fish fed dietary additives (P<0.05) while white blood cells (WBC), lymphocytes, neutrophils, hematocrit (Hct), red blood cells (RBC) were significantly enhanced (P<0.05). Fish fed with supplemented diets showed significantly enhanced antioxidant status, catalase (CAT) and superoxide dismutase (SOD). Malondialdehyde (MDA) content was significantly lower in fish fed dietary additives (P<0.05). Lactic acid bacteria (LAB) in the treatment groups were significantly increased (P<0.05). In conclusion, dietary supplementation of L. helveticus reduced detrimental effects of high stocking density on growth performance and immune response. It appears that L. helveticus can be recommended as a beneficial probiotic feed additive for rainbow trout.

Effects of dietary <italic>Lactobacillus helveticus</italic> ATC 15009 on growth performance, hematology parameters, innate immune responses, and the antioxidant status of rainbow trout (<italic>Oncorhynchus mykiss)</italic> under high rearing density

University College of Duba, Tabuk University

Health and Behavior Research Group (HBR), Psychometry and Ethology Laboratory

Pharmaceutics Department, College of Pharmacy

College of Medical Technology, The Islamic University

Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences

Department of Fisheries, Faculty of Natural Resources

Annals of Animal Science

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effects of dietary  ATC 15009 on growth performance, hematology parameters, innate immune responses, and the antioxidant status of rainbow trout ( under high rearing density

This study aimed to evaluate the effects of dietary probiotic supplementation of Lactobacillus helveticus on growth, digestive enzymes, and hematological,...

Effects of dietary Lactobacillus helveticus ATC 15009 on growth performance, hematology parameters, innate immune responses, and the antioxidant status of rainbow trout (Oncorhynchus mykiss) under high rearing density

Publicado en línea: 26 jul 2023

Páginas: 833 - 844

Recibido: 27 oct 2022

Aceptado: 04 may 2023

DOI: https://doi.org/10.2478/aoas-2023-0056

Palabras clave
immunity, fish, probiotic, antioxidant, intensive culture

© 2023 Saade Abdalkareem Jasim et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effects of dietary Lactobacillus helveticus ATC 15009 on growth performance, hematology parameters, innate immune responses, and the antioxidant status of rainbow trout (Oncorhynchus mykiss) under high rearing density

Publicado en línea: 26 jul 2023

Páginas: 833 - 844

Recibido: 27 oct 2022

Aceptado: 04 may 2023

DOI: https://doi.org/10.2478/aoas-2023-0056

Palabras claveimmunity, fish, probiotic, antioxidant, intensive culture

© 2023 Saade Abdalkareem Jasim et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Palabras clave
immunity, fish, probiotic, antioxidant, intensive culture