1. bookVolumen 22 (2022): Edición 3 (July 2022)
Detalles de la revista
Primera edición
25 Nov 2011
Calendario de la edición
4 veces al año
access type Acceso abierto

The multi-enzymes and probiotics mixture improves the growth performance, digestibility, intestinal health, and immune response of Siberian sturgeon (Acipenser baerii)

Publicado en línea: 19 Jul 2022
Volumen & Edición: Volumen 22 (2022) - Edición 3 (July 2022)
Páginas: 1063 - 1072
Recibido: 27 Sep 2021
Aceptado: 07 Dec 2021
Detalles de la revista
Primera edición
25 Nov 2011
Calendario de la edición
4 veces al año

The inclusion of exogenous digestive enzymes and probiotics is well established in the aquafeed industry. The mixture of multi-enzymes and probiotics improves the feed utilization and wellbeing of aquatic animals compared to the individual supplementation. Herein, we evaluated the exogenous multi-enzyme mixture (beta-glucanase, cellulase, alpha-amylase, protease, xylanase, and phytase) at 250 mg/kg and multi-species probiotic (Bacillus subtilis, Lactobacillus acidophilus, L. delbrueckii, L. rhamnosus, L. plantarum, and Pediococcus acidilactici; 1 × 1010 CFU/g for each bacterial strain) at 2 g/kg on the performances of Siberian sturgeon. The final weight, weight gain, SGR, and PER were markedly enhanced while the FCR was reduced in fish fed multi-enzyme and probiotics premix (P<0.05). Multi enzymes and probiotic mixture significantly increased the total body protein content (P˃0.05). Multi enzymes and probiotic mixture also improved the digestibility of crude protein, dry matter, and crude lipids nutrients (P<0.05). The count of goblet cells, microvilli diameter, microvilli length, outer muscle wall diameter, and enterocyte total absorptive surface were markedly increased (P<0.05) by dietary multienzymes and probiotics mixture. The WBCs and neutrophils showed marked improvements (P<0.05). The levels of glucose, triglycerides, blood urea nitrogen, and total bilirubin were markedly higher in fish fed the control than fish fed the multi-enzymes and probiotics mixture (P<0.05). Significantly, Siberian sturgeon-fed dietary multi-enzymes and probiotics had improved lysozyme activity, total immunoglobulin, and total protein in the skin mucus and serum samples (P<0.05). Further, the serum complement C3 and C4 was higher in fish-delivered multi-enzymes and probiotic mixture than in control (P<0.05). In conclusion, dietary probiotics synergistically enhanced the activity of multi-enzymes and resulted in increased feed utilization, nutrient digestibility, and health status of Siberian sturgeon.


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