<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The objective of the present study was to determine the seasonal changes in boar semen quality by the assessment of sperm membrane integrity, analysis of chromatin structure, assessment of oxidative stress and of apoptotic changes in spermatozoa. Semen from 16 boars (172 ejaculates) was investigated. The males were aged between 7 months and 7 years. Semen was extended with BTS diluent and stored at +17°C. During seven days of storage, the semen was subjected to standard evaluation and novel methods for semen assessment. In the autumn-winter period, the semen had higher evaluations than in the spring-summer period, but only sperm membrane integrity examination showed significantly lower (P≤0.01) percentage of moribund spermatozoa and the semen had a significantly (P≤0.05) lower (by 0.5%) percentage of sperm with damaged chromatin. Examination performed after 7-day storage showed significantly (P≤0.01) higher percentage of live spermatozoa and with high mitochondrial membrane potential for the autumn-winter period. The level of apoptotic cells was significantly (P≤0.01) lower for the autumn-winter period. Examination of sperm membrane integrity after 7 days of storage showed a lower (P≤0.05) percentage of moribund spermatozoa for the autumn-winter period. In our opinion, novel methods for sperm assessment may be used to monitor new parameters of sperm function.</p></abstract>

The objective of the present study was to determine the seasonal changes in boar semen quality by the assessment of sperm membrane integrity, analysis of chromatin structure, assessment of oxidative stress and of apoptotic changes in spermatozoa. Semen from 16 boars (172 ejaculates) was investigated. The males were aged between 7 months and 7 years. Semen was extended with BTS diluent and stored at +17°C. During seven days of storage, the semen was subjected to standard evaluation and novel methods for semen assessment. In the autumn-winter period, the semen had higher evaluations than in the spring-summer period, but only sperm membrane integrity examination showed significantly lower (P≤0.01) percentage of moribund spermatozoa and the semen had a significantly (P≤0.05) lower (by 0.5%) percentage of sperm with damaged chromatin. Examination performed after 7-day storage showed significantly (P≤0.01) higher percentage of live spermatozoa and with high mitochondrial membrane potential for the autumn-winter period. The level of apoptotic cells was significantly (P≤0.01) lower for the autumn-winter period. Examination of sperm membrane integrity after 7 days of storage showed a lower (P≤0.05) percentage of moribund spermatozoa for the autumn-winter period. In our opinion, novel methods for sperm assessment may be used to monitor new parameters of sperm function.

Use of Novel Methods to Assess Seasonal Differences in the Quality of Boar Semen Stored Up to 7 Days at 17°C

Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production

Annals of Animal Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The objective of the present study was to determine the seasonal changes in boar semen quality by the assessment of sperm membrane integrity, analysis of...

Use of Novel Methods to Assess Seasonal Differences in the Quality of Boar Semen Stored Up to 7 Days at 17°C

Publicado en línea: 30 oct 2019

Páginas: 967 - 978

Recibido: 20 sept 2018

Aceptado: 16 may 2019

DOI: https://doi.org/10.2478/aoas-2019-0035

Palabras clave
boar semen, membrane integrity, chromatin structure, oxidative stress, apoptotic changes

© 2019 Barbara Szczęśniak-Fabiańczyk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Use of Novel Methods to Assess Seasonal Differences in the Quality of Boar Semen Stored Up to 7 Days at 17°C

Publicado en línea: 30 oct 2019

Páginas: 967 - 978

Recibido: 20 sept 2018

Aceptado: 16 may 2019

DOI: https://doi.org/10.2478/aoas-2019-0035

Palabras claveboar semen, membrane integrity, chromatin structure, oxidative stress, apoptotic changes

© 2019 Barbara Szczęśniak-Fabiańczyk et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Palabras clave
boar semen, membrane integrity, chromatin structure, oxidative stress, apoptotic changes